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Anim Reprod Sci ; 146(3-4): 165-9, 2014 May.
Article in English | MEDLINE | ID: mdl-24725536

ABSTRACT

This study evaluated associations between the presence of protein bands in ram seminal plasma and the quality of sperm frozen with distinct extenders. Ejaculates were frozen in a Tris-egg yolk based extender, including either 5% glycerol or 100mM trehalose. Seminal plasma samples were submitted to unidimensional electrophoresis. Pre-freezing and post-thawing sperm quality was similar between extenders (P>0.05). A total of 26 bands were identified in ram seminal plasma. Pre-freezing sperm motility was increased when the 15, 19 and 80kDa bands were present in seminal plasma (P<0.05). The presence of an 11kDa band in seminal plasma was associated with reduced pre-freezing membrane integrity (P<0.05). After thawing, both sperm motility and membrane integrity were reduced when a 24kDa band was present in seminal plasma (P<0.05). Post-thawing acrosome integrity was greater in the presence of a 31kDa band in seminal plasma (P<0.05). Regardless of the cryoprotectant included in the freezing extender, these six bands may be potential markers for ram sperm tolerance to freezing.


Subject(s)
Cryopreservation/veterinary , Proteins/chemistry , Semen Preservation/veterinary , Semen/chemistry , Sheep/physiology , Spermatozoa/physiology , Animals , Cell Survival , Freezing , Male , Semen Preservation/methods , Spermatozoa/cytology
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