ABSTRACT
Pathogen virulence exists on a continuum. The strategies that drive symptomatic or asymptomatic infections remain largely unknown. We took advantage of the concept of lethal dose 50 (LD50) to ask which component of individual non-genetic variation between hosts defines whether they survive or succumb to infection. Using the enteric pathogen Citrobacter, we found no difference in pathogen burdens between healthy and symptomatic populations. Iron metabolism-related genes were induced in asymptomatic hosts compared to symptomatic or naive mice. Dietary iron conferred complete protection without influencing pathogen burdens, even at 1000× the lethal dose of Citrobacter. Dietary iron induced insulin resistance, increasing glucose levels in the intestine that were necessary and sufficient to suppress pathogen virulence. A short course of dietary iron drove the selection of attenuated Citrobacter strains that can transmit and asymptomatically colonize naive hosts, demonstrating that environmental factors and cooperative metabolic strategies can drive conversion of pathogens toward commensalism.
Subject(s)
Host-Pathogen Interactions/physiology , Iron/metabolism , Virulence/physiology , Animals , Asymptomatic Infections , Citrobacter rodentium/metabolism , Citrobacter rodentium/pathogenicity , Colitis/drug therapy , Colitis/metabolism , Colon/microbiology , Dietary Supplements , Enterobacteriaceae Infections/drug therapy , Female , Insulin Resistance/physiology , Intestine, Small/microbiology , Iron/pharmacology , Lethal Dose 50 , Male , Mice , Mice, Inbred C3H , Mice, Inbred DBAABSTRACT
Sickness-induced anorexia is a conserved behavior induced during infections. Here, we report that an intestinal pathogen, Salmonella Typhimurium, inhibits anorexia by manipulating the gut-brain axis. Inhibition of inflammasome activation by the S. Typhimurium effector, SlrP, prevented anorexia caused by IL-1ß-mediated signaling to the hypothalamus via the vagus nerve. Rather than compromising host defenses, pathogen-mediated inhibition of anorexia increased host survival. SlrP-mediated inhibition of anorexia prevented invasion and systemic infection by wild-type S. Typhimurium, reducing virulence while increasing transmission to new hosts, suggesting that there are trade-offs between transmission and virulence. These results clarify the complex and contextual role of anorexia in host-pathogen interactions and suggest that microbes have evolved mechanisms to modulate sickness-induced behaviors to promote health of their host and their transmission at the expense of virulence.
Subject(s)
Anorexia/microbiology , Salmonella Infections/microbiology , Salmonella Infections/transmission , Salmonella typhimurium/pathogenicity , Animals , Bacterial Proteins/metabolism , Host-Pathogen Interactions , Humans , Inflammasomes/immunology , Interleukin-1beta/immunology , Mice , Mice, Inbred C57BL , Neural Pathways , Salmonella Infections/immunology , Salmonella typhimurium/physiology , Specific Pathogen-Free Organisms , VirulenceABSTRACT
Physiological responses that occur during infection are most often thought of in terms of effectors of microbial destruction through the execution of resistance mechanisms, due to a direct action of the microbe, or are maladaptive consequences of host-pathogen interplay. However, an examination of the cellular and organ-level consequences of one such response, thermoregulation that leads to fever or hypothermia, reveals that these actions cannot be readily explained within the traditional paradigms of microbial killing or maladaptive consequences of host-pathogen interactions. In this review, the concept of disease tolerance is applied to thermoregulation during infection, inflammation and trauma, and we discuss the physiological consequences of thermoregulation during disease including tissue susceptibility to damage, inflammation, behavior and toxin neutralization.
Subject(s)
Adaptation, Physiological , Body Temperature Regulation , Disease Resistance , Host-Pathogen Interactions , Animals , Behavior , Disease Susceptibility , Energy Metabolism , Gene Expression Regulation , Humans , Immunomodulation , Inflammation/etiology , Inflammation/metabolism , Organ SpecificityABSTRACT
Infections and inflammation can lead to cachexia and wasting of skeletal muscle and fat tissue by as yet poorly understood mechanisms. We observed that gut colonization of mice by a strain of Escherichia coli prevents wasting triggered by infections or physical damage to the intestine. During intestinal infection with the pathogen Salmonella Typhimurium or pneumonic infection with Burkholderia thailandensis, the presence of this E. coli did not alter changes in host metabolism, caloric uptake, or inflammation but instead sustained signaling of the insulin-like growth factor 1/phosphatidylinositol 3-kinase/AKT pathway in skeletal muscle, which is required for prevention of muscle wasting. This effect was dependent on engagement of the NLRC4 inflammasome. Therefore, this commensal promotes tolerance to diverse diseases.