ABSTRACT
The preservation of the nuclear polarization of hydrogen atoms during the recombination to molecules was observed on different surface materials in the temperature range from 45 to 100 K and for magnetic fields up to 1 T. On a gold and a fused quartz surface, the expected molecular polarization of about 50% or lower of the atomic polarization was measured, while a surface layer of perfluoropolyether (Fomblin) shows a nearly complete preservation (at least 97%) of the atomic polarization during the recombination process. Further experiments have the possibility of storing polarized deuterium molecules and to use them in nuclear-fusion installations. Another application might be the production of polarized substances for enhanced NMR techniques.
ABSTRACT
BACKGROUND: We investigated the utility of cytologic studies (including follow-up studies) in our specialty in determining the prevalence of human papillomavirus, specifically in the larynx. We utilized Papanicolaou's method of exfoliative cytology and in situ hybridization of the culture (biotin-marked DNA probes). PATIENTS: 486 patients with chronic hyperplastic laryngitis and 74 patients with papilloma were enrolled in the study. RESULTS: In 198 typings in 132 patients (59 patients with papilloma, 41 with chronic hyperplastic laryngitis, 17 with cancer, and 15 healthy), we found distributions of the individual types that were independent of the diagnosis. Particularly often, we encountered human papillomavirus types 18 and 31 in the presence of dysplasia, human papillomavirus type 16 in the presence of cancer, and type 11 in general. CONCLUSIONS: Cytologic examination and human papillomavirus typing of the culture permit us to define a third risk category in addition to the known risk groups smokers and dysplasia patients. Besides this, cytologic follow-up studies can provide further insight into the behavior of preneoplastic epithelial changes.
Subject(s)
Laryngeal Neoplasms/pathology , Laryngitis/pathology , Papilloma/pathology , Papillomaviridae/isolation & purification , Papillomavirus Infections/pathology , Precancerous Conditions/pathology , Tumor Virus Infections/pathology , Adult , Aged , Biopsy , Cell Transformation, Neoplastic/pathology , Chronic Disease , Female , Follow-Up Studies , Humans , Hyperplasia , Laryngeal Neoplasms/virology , Laryngitis/virology , Larynx/pathology , Larynx/virology , Male , Middle Aged , Papilloma/virology , Papillomavirus Infections/virology , Precancerous Conditions/virology , Tumor Virus Infections/virologyABSTRACT
Virus induced changes occur not so rarely in combination with precancerous and cancerous lesions. We compared the cytological and histological findings. The increasing frequency of this event is demonstrated by an analysis of the material seen in our laboratory, smears from more than 120,000 patients per year. It was possible to verify the viral etiology by the electron microscopical demonstration of virus particles.
Subject(s)
Inclusion Bodies, Viral/ultrastructure , Tumor Virus Infections/pathology , Uterine Cervical Neoplasms/pathology , Vaginal Smears , Carcinoma in Situ/pathology , Cervix Uteri/pathology , Condylomata Acuminata/pathology , Epithelium/pathology , Female , Humans , Microscopy, Electron , Neoplasm Invasiveness , Papillomaviridae/ultrastructure , Uterine Cervical Dysplasia/pathologyABSTRACT
The three cellular components of endocervical epithelium were studied by means of optical light microscopy and electron microscopy. Particular attention was given to cytochemical responses to attempts by which to detect mucus in cylindrical epithelium. The beaker cells were grouped by stages by means of electron microscopy.