ABSTRACT
Sonographic examinations were carried out in patients who had been subjected to supratentorial and infratentorial surgery. A Computer tomogram was prepared simultaneously with the sonogram. In all cases there was a high degree of congruence between the CT and sonogram findings.
Subject(s)
Brain Diseases/surgery , Echocardiography/methods , Trephining , Brain/pathology , Brain Diseases/pathology , Cerebral Ventricles/pathology , Humans , Postoperative Complications/pathology , Tomography, X-Ray ComputedABSTRACT
Nuclear and cytosolic androgen receptor concentrations in tissues of human benign prostatic hypertrophy (BPH) were determined by use of methyltrienolone (R-1881) and 7 alpha,17 alpha-dimethyl-19-nortestosterone (DMNT) as radiolabeled ligands. Cytosolic R-1881-binding sites were 46.1 +/- 43 fmol/mg protein and nuclear R-1881-binding sites were 51.8 +/- 42 fmol/mg protein. DMNT-binding sites in cytosol were 44.3 +/- 38 fmol/mg protein and in nuclear extract 73.4 +/- 64 fmol/mg protein. No significant correlation was found between the number of R-1881- and DMNT-binding sites in either cytosol or nuclear extracts. Cytosolic or nuclear androgen receptor content was not significantly correlated with the percentage of epithelial or stromal cells as determined from the corresponding histological sections. In BPH tissue with marked cystic degeneration, very low androgen receptor levels were found.
Subject(s)
Prostatic Hyperplasia/metabolism , Receptors, Androgen/metabolism , Cell Nucleus/metabolism , Cytoplasm/metabolism , Cytosol/metabolism , Epithelium/metabolism , Estrenes/metabolism , Humans , Male , Metribolone , Nandrolone/analogs & derivatives , Nandrolone/metabolism , Promegestone/metabolism , Prostate/metabolism , Radioligand Assay , Receptors, Progesterone/metabolism , Testosterone Congeners/metabolismABSTRACT
A simple two-step procedure is described for the purification of rat liver ATP citrate lyase with an overall yield of upt to 70%. In binding experiments on Blue Sepharose CL-6B (an affinity gel for nucleotide-specific enzymes) it is observed that ATP citrate lyase recognizes ATP and CoA as well as ADP, 3'-AMP, and NADP, but not 5'-AMP, NAD, or adenosine. It is further demonstrated that ATP citrate lyase is activated by various anions including chloride, hydrogen carbonate, and acetate. The highest specific activity of the enzyme is found in adipose tissue of various origins. Activities are also present in the adrenals, in liver, and intestine, in brain, and in lung. No measurable enzyme activity is found in heart, skeletal muscle, and in the kidneys.
