Subject(s)
Leukemia, Myeloid, Acute/genetics , Leukemia, Myeloid, Acute/mortality , NK Cell Lectin-Like Receptor Subfamily D/genetics , Neural Cell Adhesion Molecules/genetics , Adolescent , Child , Child, Preschool , Female , Gene Expression , Humans , Infant , Killer Cells, Natural/pathology , Male , NK Cell Lectin-Like Receptor Subfamily D/analysis , Neural Cell Adhesion Molecules/analysis , Survival RateABSTRACT
Sickle cell disease adds relevant problems to the physical, emotional and social changes that normally occur during adolescence. Specific physical characteristics and complications of the disease can hinder the social and emotional adjustment of the affected teenagers. The cooperation between the physician and the parents is essential in order to assist the teenager to the difficulties of this critical phase of adolescence. Recommendations are best offered in the form of education of the adolescent about the disease, education of the family and the school about the needs of the adolescent, and the preparation of the adolescent for the transition to the adult life and adult medical care.
Subject(s)
Anemia, Sickle Cell/psychology , Adolescent , Humans , Psychology, AdolescentABSTRACT
There is a known association between lymphoid malignancy and Hodgkin's disease (HD), but the development of HD in children who have been treated for leukemia or lymphoma is very uncommon. Hodgkin's disease is, after retinoblastoma, the most common primary tumor that is associated with development of second malignant neoplasm. For reasons that remain to be determined, HD is very rare as a second malignancy [1, 2, 3]. We report the case of a eight-year-old girl who developed HD 6 years after treatment for common acute lymphoblastic leukemia (ALL). This case prompted us to review the published literature for cases of secondary HD in childhood. Our experience suggests that we should follow strictly our patients with ALL and be ready to intervene with invasive diagnostic procedures at the least suspicion of a second or recurrent neoplasm. The most frequent causes of second tumors are radiotherapy, genetic susceptibility and prior treatment with certain chemotherapeutic agents, such as nitrogen mustards. It is likely that any type of immunodeficiency, even without symptoms, might play a role in the development of second tumors in childhood.
Subject(s)
Hodgkin Disease/diagnosis , Antineoplastic Combined Chemotherapy Protocols/administration & dosage , Disease-Free Survival , Female , Hodgkin Disease/etiology , Humans , Infant , Neoplasms, Second Primary/diagnosis , Neoplasms, Second Primary/etiology , Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy , Tomography, Emission-Computed, Single-PhotonABSTRACT
BACKGROUND: Children with leukemia undergo painful procedures such as lumbar puncture and bone marrow aspiration. To overcome pain, certain units offer total anesthesia; others offer generic support; others offer no preparation at all. Since September, 1997, we have provided leukemic children with art therapy (AT), a nonverbal and creative modality that develops coping skills. Our goal is to prevent anxiety and fear during painful interventions as well as prolonged emotional distress. PROCEDURE: We treated 32 children aged 2-14 years. The modes of AT before, during, and after the punctures were as follows: clinical dialogue to calm children and help them cope with painful procedures; visual imagination to activate alternative thought processes and decrease the attention towards overwhelming reality and raise the peripheral sensitivity gate; medical play to clarify illness, eliminate doubts, and offer control over threatening reality; structured drawing to contain anxiety by offering a structured, predictable reality (the drawing) that was controllable by children; free drawing to allow children to externalize confusion and fears; and dramatization to help children accept and reconcile themselves to body changes. RESULTS: Children hospitalized before September, 1997, exhibited resistance and anxiety during and after painful procedures. By contrast, children provided with AT from the first hospitalization exhibited collaborative behavior. They or their parents asked for AT when the intervention had to be repeated. Parents declared themselves better able to manage the painful procedures when AT was offered. CONCLUSION: AT was shown to be a useful intervention that can prevent permanent trauma and support children and parents during intrusive interventions.
Subject(s)
Art Therapy/methods , Bone Marrow Purging/psychology , Leukemia/psychology , Pain/psychology , Spinal Puncture/psychology , Adaptation, Psychological , Adolescent , Age Factors , Bone Marrow Purging/adverse effects , Child , Child, Hospitalized/psychology , Child, Preschool , Humans , Infant , Leukemia/therapy , Pain/etiology , Pilot Projects , Spinal Puncture/adverse effectsABSTRACT
BACKGROUND AND OBJECTIVES: The occurrence of Hb CS is usually limited to the geographic area which includes Southern China and South East Asia. In 1968 Hb CS was also found to occur in the Mediterranean area where it was originally described as Hb Athens. We investigated the independent origin of these termination codon mutations of the alpha 2-globin gene by determining the alpha-cluster haplotype and comparing the hematologic data from Hb CS-Hb H patients and their family members. DESIGN AND METHODS: We studied one Hb CS-Hb H patient of Greek origin and a Sicilian family in which one individual was affected by Hb CS-Hb H. The haplotype of the Hb CS allele was determined and compared to the haplotype of an Hb CS-Hb H individual of Chinese origin. RESULTS: The haplotype found for the Greek and Sicilian Hb CS was the same but differed significantly from the Asiatic Hb CS mutation. INTERPRETATION AND CONCLUSIONS: The Hb CS mutation found in both Mediterranean patients arose independently in the Mediterranean area. The difference in clinical manifestation of the Hb CS-Hb H disease in both patients is less common but consistent with similar variation in the clinical expression of analogous Hb Icaria-Hb H disease patients.
Subject(s)
Hemoglobins, Abnormal/genetics , China , Codon, Terminator/genetics , Family Health , Female , Greece , Haplotypes , Humans , Male , Point Mutation , SicilyABSTRACT
We studied 15 Sicilian subjects with Hb H disease correlating clinical examinations with hematological and molecular data. Seven different alpha-tha1 mutations were identified: four deletion types (--MED --CAL, -alpha3.7, -alpha4.2) and three nondeletion types (alpha(Ncol)alpha, alpha(Hph)alpha, alphaCSalpha). All the patients had a zero-gene chromosome (--MED or --CAL), while the third alpha gene was deleted (-alpha3.7, -alpha4.2) or inactive (alpha(Ncol)alpha, alpha(Hph)alpha, alphaCSalpha). In patients with the nondeletion genotype the analysis of hematological values revealed lower levels of RBC and Hb A2 and significantly higher levels of Hb H. The clinical variability was remarkable, ranging from totally asymptomatic conditions, casually diagnosed, to severe thalassemia intermedia with marked hemolytic crises, liver and spleen enlargement and the necessity for frequent transfusions. The genotype did not justify the gravity of the phenotype in every case, and the differences in clinical manifestations, also notable, are not easily explainable in subjects who apparently have the same genotype.
Subject(s)
Genotype , Phenotype , alpha-Thalassemia/genetics , Adolescent , Adult , Child , Child, Preschool , DNA Mutational Analysis , Family Health , Female , Gene Deletion , Globins/genetics , Hemoglobin H/chemistry , Hemoglobin H/genetics , Humans , Male , Middle Aged , Mutation/genetics , Sicily/epidemiology , alpha-Thalassemia/blood , alpha-Thalassemia/complicationsABSTRACT
BACKGROUND: This study describes the incidence of acute neurotoxicity (NT) in children with B-lineage acute lymphoblastic leukemia (ALL) treated with three intermediate risk protocols that differ in the intensity of central nervous system (CNS) "prophylaxis. " PROCEDURE: A total of 122 patients (64 boys; median age 5.3 years) with B-lineage ALL without CNS leukemia diagnosed between February 1987 and December 1997 were enrolled in the intermediate risk (IR) protocols: Associazione Italiana di Ematologia ed Oncologia Pediatrica (AIEOP)-ALL 87 (n = 33), 91 (n = 51), and 95 (n = 38). Presymptomatic CNS therapy consisted of intrathecal methotrexate (six doses) and cranial irradiation (18 Gy) in the IR AIEOP 87 study, and extended triple intrathecal therapy with methotrexate, cytarabine, and prednisone depending on age in the IR AIEOP-ALL 91 and 95 protocols (20 and 17 total doses, respectively). World Health Organization (WHO) grade 4 acute neurotoxicity criteria were employed. Patients with neurologic symptoms, in addition to physical examination, underwent EEG, computed tomography (CT) and/or magnetic resonance imaging (MRI), and lumbar puncture to exclude CNS leukemia and infection. RESULTS: Acute NT was not reported in AIEOP-ALL 87 treated patients, but we observed acute NT in 3 out of 51 (5.8%) AIEOP-ALL 91 patients, and in 7 out of 38 (18.4%) AIEOP-ALL 95 patients. CONCLUSIONS: There was an increased incidence of acute NT in our patients with ALL treated with current intermediate risk protocols. The intensification of treatment, however, bettered event free survival (EFS) to 58%, 72% and 85% in IR AIEOP 87, 91 and 95 studies, respectively.
Subject(s)
Antimetabolites, Antineoplastic/adverse effects , Antineoplastic Combined Chemotherapy Protocols/adverse effects , Burkitt Lymphoma/drug therapy , Central Nervous System Diseases/chemically induced , Methotrexate/adverse effects , Adolescent , Anti-Inflammatory Agents/adverse effects , Burkitt Lymphoma/mortality , Central Nervous System Diseases/epidemiology , Child , Child, Preschool , Combined Modality Therapy , Cytarabine/adverse effects , Disease-Free Survival , Female , Humans , Hydrocortisone/adverse effects , Incidence , Infant , Injections, Spinal , Italy/epidemiology , Male , Radiotherapy, Adjuvant , Retrospective StudiesSubject(s)
Hospitals, University/organization & administration , Oncology Service, Hospital/organization & administration , Pediatrics/organization & administration , Adolescent , Child , Child, Preschool , Hematologic Neoplasms/therapy , Humans , Infant , Infant, Newborn , Italy , Neoplasms/therapyABSTRACT
The aim of this study is to verify whether there are deletions in mitochondrial DNA (mtDNA) and disorders in oxidative phosphorylation (Ox-phos) complexes in the pathogenesis of secondary Fanconi syndrome (FS). We studied 18 children with tumors who were previously treated with chemotherapy and were off therapy for at least 1 year. All the children had normal renal function at diagnosis. Only 4 children received ifosfamide (IFO) and platinum compounds. We evaluated renal function, Ox-phos activity measured on platelets, and mtDNA extracted from platelets for all patients. Only 2 patients, both treated with IFO and carboplatinum (CARBO) for Wilms' tumor and germ-cell tumor, respectively, developed FS 1 and 3 years after termination of therapy. They had decreased activities of Ox-phos that were statistically significant only for nicotinamide adenine dinucleotide (NAD)-reduced cytochrome-c reductase and cytochrome-c oxidase and specific and unidentified deletions in mtDNA that were not maternally inherited. Our data suggest that treatment with IFO and CARBO might be responsible for deletions in mtDNA, decreased activity of Ox-phos, and impaired rates of transport of D-glucose, phosphate, and amino acids.
Subject(s)
Antineoplastic Agents/adverse effects , DNA, Mitochondrial/genetics , Fanconi Syndrome/chemically induced , Oxidative Phosphorylation/drug effects , Antineoplastic Agents/therapeutic use , Antineoplastic Agents, Alkylating/adverse effects , Antineoplastic Agents, Alkylating/therapeutic use , Blotting, Southern , Carboplatin/adverse effects , Carboplatin/therapeutic use , Case-Control Studies , Child , Child, Preschool , DNA, Mitochondrial/drug effects , Female , Humans , Ifosfamide/adverse effects , Ifosfamide/therapeutic use , Male , Polymerase Chain ReactionABSTRACT
Chitotriosidase, a macrophage marker, which is extremely increased in plasma of Gaucher patients, was measured in patients with beta-thalassemia, an haematological disorder characterized by the genetic defect of beta-globin chains synthesis resulting in unproductive erythropoiesis and enormous expansion of the reticuloendothelial system. Plasma chitotriosidase was increased to a variable extent in 13 of 70 patients with beta-thalassemia major treated with the intense transfusion regimen and iron chelation therapy. It was normal in 22 and slightly elevated in 3 subjects with beta-thalassemia intermedia which were not transfused. The highest levels of plasma chitotriosidase, as high as in Gaucher patients, were found in 7 (10%) of the beta-thalassemia major patients which also had the highest degree of iron overload as judged by their serum ferritin level (> 3000 ng/ml), high SGPT level and elevated urinary iron excretion. To our knowledge, beta-thalassemia is hitherto the only disorder in which an increase of plasma chitotriosidase, comparable to that seen in Gaucher disease, may occur. The increase of plasma chitotriosidase activity in beta-thalassemia patients with high iron overload, could be related to an iron mediated damage to the lysosomal apparatus. In addition, similarly to Gaucher disease, the increased chitotriosidase production in beta-thalassemia might reflect macrophage activation probably related to the intracellular iron overload, storage of erythrocytes membrane break-down products and oxidation of excess alpha-hemoglobin subunits. Further studies are required to define the role of chitotriosidase evaluation to assess the efficacy of chelation therapy in reducing the macrophage activation due to intracellular iron overload in beta-thalassemia.
Subject(s)
Hexosaminidases/blood , beta-Thalassemia/blood , Adolescent , Adult , Child , Child, Preschool , Female , Gaucher Disease/blood , Globins/genetics , Globins/metabolism , Humans , Iron/metabolism , MaleABSTRACT
Although most relapses of childhood acute lymphoblastic leukemia (ALL) occur 24-36 months after first CR has been achieved, few patients relapse 5 or more years after CR achievement. The assessment of clonality has proved to be useful in determining whether even those very late events represent the reoccurrence of the original clone or alternatively a secondary leukemia. To gain further information on clonal stability in such late relapse, we performed detailed comparative Southern blotting and PCR analyses of TcRdelta and TcRgamma gene rearrangements in five ALL at presentation and subsequent relapse which occurred more than 5 years after diagnosis. At least one stable rearranged allele of the TcRdelta and TcRgamma loci was traced in all cases at presentation and clinical relapse despite a wide heterogeneity of the pattern of rearrangements. Our study extends to a larger series of patients previous findings which have sought to analyze the phenomenon of clonal evolution in children relapsed after more than 5 years of CCR. With respect to the potential pitfalls in monitoring minimal residual disease in childhood ALL for the presence of clonal evolution, our results highlight the combination of two target genes (such as TcRgamma and TcRdelta) as a tool to reduce false negative MRD results.
Subject(s)
Gene Rearrangement, T-Lymphocyte , Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics , Blotting, Southern , Child , Child, Preschool , Humans , Male , Pilot Projects , Polymerase Chain Reaction , Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis , RecurrenceABSTRACT
BACKGROUND AND OBJECTIVE: The present study was designed to determine the distribution and severity of sickle cell disease (SCD) in Italy. DESIGN AND METHODS: A questionnaire, requesting information about the cases of sickle cell disease that had been seen during previous years, was sent to all Italian centers of Pediatrics and Hematology. The questionnaire was simple and required personal, hematologic and clinical information. RESULTS: A total of 696 cases were reported. The distribution of registered patients shows that, although the S gene originated mostly in Sicily and Southern Italy, 20% of patients with SCD now live in Central and Northern Italy. The types of SCD reported were as follows: compound heterozygotes HbS-beta thalassemia, (S-Th, 518 cases); homozygotes for HbS, (S-S, 149 cases); compound heterozygotes HbS and another abnormal hemoglobin (21 cases). The population of patients with SCD is younger than the general Italian population. More than 90% of patients have had no crises or only a limited number, namely, up to 6/year. Infections ranged between 0 and 6/year. Splenomegaly was reported in 28% and 80% of adult patients with S-S and S-Th, respectively. The prevalence of gallstones was 48%. INTERPRETATION AND CONCLUSIONS: The survey established that 1) sickle cell disease is widely distributed in Italy; 2) while the clinical spectrum is extremely variable, severe forms are infrequent.
Subject(s)
Anemia, Sickle Cell , Adolescent , Adult , Aged , Anemia, Sickle Cell/epidemiology , Anemia, Sickle Cell/genetics , Anemia, Sickle Cell/physiopathology , Child , Child, Preschool , Female , Health Surveys , Humans , Italy/epidemiology , Male , Middle Aged , Surveys and QuestionnairesABSTRACT
This article describes a preliminary study of screening/diagnostic instruments for prediction for large-scale application in the military field at the Neuropsychiatric Department of the Military Hospital of Legal Medicine of Verona and for the prevention of self-destructive behaviors, particularly through the use of drugs. 170 subjects divided into three subsamples were examined. The first subsample was characterized by a strong tendency towards normalcy, the second by a strong tendency towards pathology, and the third by a great variety of expressions of psychological and social problems, which were not necessarily related to drug use. These subjects were administered a questionnaire designed according to Squashing Theory principles (Buscema, 1994a). Answers were processed by an Artificial Neural Network created by Semeion in Rome (Buscema, 1996) and were compared with a standard clinical psychiatric assessment report and with the results of psychodiagnostic tests. Results document ANNs' remarkable ability to recognize subjects with declared, in exordium and "at risk" pathological behaviors. Blind results on learning and trial samples show a very high predictive capacity (over 90%). A comparison with the examined subjects' clinical report and the results of the first follow-up also document very high agreements. The broad variation of answers obtained in the third subsample allows further methodological reflections on the contribution of Artificial Neural Networks and Squashing Theory to the study of deviance, for both sociologists and clinicians, and not only for those in the field of drug addiction.
Subject(s)
Artificial Intelligence , Forensic Medicine , Heroin Dependence/diagnosis , Medical Informatics Applications , Military Personnel/psychology , Neural Networks, Computer , Adult , Follow-Up Studies , Heroin Dependence/epidemiology , Hospitals, Military , Humans , Italy/epidemiology , Male , Pilot Projects , Prospective Studies , Surveys and Questionnaires , Time FactorsABSTRACT
The molecular basis of acute lymphoblastic leukemia (ALL) of both B-cell and T-cell lineages seems better understood using polymerase chain reaction (PCR) methods. The analysis of clone-specific junctional regions of rearranged genes for both Immunoglobulin (Ig H) and T-cell receptor (TcR) is the most sensitive tool for detection of minimal residual disease (MRD) in ALL. Because of the heterogeneity of all ALL patients examined in several studies, the detection of MRD at different times of treatment has not as yet been correlated with disease outcome. In contrast, T-ALL is a homogeneous disease characterized by expansion of a single clone showing a specific Rearranged junctional region of TcR delta and/or gamma genes. The use of a clone-specific probe allows detection of residual leukemia throughout treatment. However, 60 % of patients with T-ALL relapse during treatment or towards the end of therapy, with resurgence of the original leukemic clone. It is possible that the detection of MRD at a specific time-point after diagnosis, as well as at the beginning of maintenance, may help to identify a group of T-ALL patients at high risk of relapse. The correlation between detection of MRD and treatment phase may be used in the future to evaluate whether treatment regimens can be improved allowing for stratification, based on PCR-mediated detection of MRD.
Subject(s)
Leukemia-Lymphoma, Adult T-Cell/diagnosis , Neoplasm, Residual/diagnosis , Proto-Oncogene Proteins , Transcription Factors , Basic Helix-Loop-Helix Transcription Factors , DNA-Binding Proteins/genetics , Disease-Free Survival , Genes, T-Cell Receptor beta/genetics , Genes, T-Cell Receptor delta/genetics , Genes, T-Cell Receptor gamma/genetics , Humans , Leukemia-Lymphoma, Adult T-Cell/genetics , Leukemia-Lymphoma, Adult T-Cell/mortality , Neoplasm, Residual/genetics , Neoplasm, Residual/mortality , Polymerase Chain Reaction , Receptors, Antigen, T-Cell/genetics , T-Cell Acute Lymphocytic Leukemia Protein 1 , Treatment OutcomeABSTRACT
Expression of fetal hemoglobin (Hb F) is under polygenic control involving determinants both linked and unlinked to the beta-globin gene cluster on chromosome 11. Variations in the DNase I-hypersensitive site 2 of the locus control region (LCR-HS2) and a C --> T change at position -158 from the Ggamma-gene (detected as an XmnI polymorphism) correlate with the high level of Hb F expression in patients with sickle-cell anemia and beta-thalassemia. Interpretation of data under these conditions of anemic stress is difficult because the preferential survival of Hb F-containing erythrocytes (F-cells) may not reflect the true status of Hb F expression. We investigated the relationship between these markers and Hb F expression in terms of F-cell levels in 48 unrelated non-anemic AS heterozygotes from Sicily. The betaS-chromosome of all these individuals was of the Benin haplotype and they differed only by their betaA chromosomes. We demonstrate that F-cell expression is more strongly associated with LCR-HS2 polymorphism than with XmnI polymorphism. The observed association between XmnI polymorphism and Hb F expression is very likely to be due to linkage disequilibrium with LCR-HS2 sequences.
Subject(s)
Anemia, Sickle Cell/genetics , Fetal Hemoglobin/genetics , Globins/genetics , Multigene Family , Polymorphism, Genetic , Adult , Aged , Anemia, Sickle Cell/metabolism , Anemia, Sickle Cell/pathology , DNA/analysis , Erythrocyte Count , Female , Fetal Hemoglobin/biosynthesis , Gene Expression Regulation , Genetic Markers , Haplotypes/genetics , Humans , Male , Middle Aged , Polymerase Chain Reaction , Site-Specific DNA-Methyltransferase (Adenine-Specific)/geneticsABSTRACT
The aims of this study were twofold: (1) to assess the marrow of patients with T-lineage acute lymphoblastic leukemia (T-ALL) for the presence of molecular residual disease (MRD) at different times after diagnosis and determine its value as a prognostic indicator; and (2) to compare the sensitivity, rapidity, and reliability of two methods for routine clinical detection of rearranged T-cell receptor (TCR). Marrow aspirates from 23 patients with T-ALL diagnosed consecutively from 1982 to 1994 at the Division of Pediatric Hematology and Oncology, University of Catania, Italy, were obtained at diagnosis, at the end of induction therapy (6 to 7 weeks after diagnosis), at consolidation and/or reinforced reinduction (12 to 15 weeks after diagnosis), at the beginning of maintenance therapy (34 to 40 weeks after diagnosis), and at the end of therapy (96 to 104 weeks after diagnosis). DNA from the patients' marrow was screened using the polymerase chain reaction (PCR) for the four most common TCR delta rearrangements in T-ALL (Vdelta1 Jdelta1, Vdelta2 Jdelta1, Vdelta3 Jdelta1, and Ddelta2 Jdelta1) and, when negative, further tested for the presence of other possible TCR delta and TCR gamma rearrangements. After identification of junctional rearrangements involving V, D, and J segments by DNA sequencing, clone-specific oligonucleotide probes 5' end-labeled either with fluorescein or with [gamma-32P]ATP were used for heminested PCR or dot hybridization of PCR products of marrows from patients in clinical remission. For 17 patients with samples that were informative at the molecular level, the estimated relapse-free survival (RFS) at 5 years was 48.6% (+/-12%). The sensitivity and specificity for detection of MRD relating to the outcome were 100% and 88.9% for the heminested fluorescence PCR and 71.4% and 88.9% for Southern/dot blot hybridization, respectively. Predictive negative and positive values were 100% and 90.7% for heminested fluorescence PCR, respectively. The probability of RFS based on evidence of MRD as detected by heminested fluorescence PCR at the time of initiation of maintenance therapy was 100% and 0% for MRD-negative and MRD-positive patients, respectively. Thus, the presence of MRD at the beginning of maintenance therapy is a strong predictor of poor outcome, and the molecular detection of MRD at that time might represent the basis for a therapeutic decision about such patients. By contrast, the absence of MRD at any time after initiation of treatment strongly correlates with a favorable outcome. The heminested fluorescence PCR appears to be more accurate and more rapid than other previously used methods for the detection of residual leukemia.
Subject(s)
Biomarkers, Tumor/analysis , Blotting, Southern/methods , DNA, Neoplasm/analysis , Gene Rearrangement, delta-Chain T-Cell Antigen Receptor , Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor , Leukemia-Lymphoma, Adult T-Cell/pathology , Neoplastic Stem Cells/chemistry , Nucleic Acid Hybridization/methods , Polymerase Chain Reaction/methods , Receptors, Antigen, T-Cell, gamma-delta/genetics , Adolescent , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Asparaginase/administration & dosage , Bone Marrow/pathology , Child , Child, Preschool , Cohort Studies , Cyclophosphamide/administration & dosage , Cytarabine/administration & dosage , Daunorubicin/administration & dosage , Disease-Free Survival , Evaluation Studies as Topic , Female , Fluorometry , Humans , Immunophenotyping , Infant , Italy/epidemiology , Leucovorin/administration & dosage , Leukemia-Lymphoma, Adult T-Cell/drug therapy , Leukemia-Lymphoma, Adult T-Cell/genetics , Leukemia-Lymphoma, Adult T-Cell/mortality , Male , Mercaptopurine/administration & dosage , Methotrexate/administration & dosage , Neoplasm, Residual , Neoplastic Stem Cells/pathology , Prednisone/administration & dosage , Prognosis , Prospective Studies , Receptors, Antigen, T-Cell, gamma-delta/analysis , Remission Induction , Sensitivity and Specificity , Treatment Outcome , Vincristine/administration & dosageSubject(s)
Biomarkers, Tumor/analysis , Neoplasm Proteins/analysis , Oncogene Proteins, Fusion , Precursor Cell Lymphoblastic Leukemia-Lymphoma/pathology , Child , Core Binding Factor Alpha 2 Subunit , Humans , Neoplasm, Residual , Polymerase Chain Reaction , RNA, Neoplasm/analysis , Remission InductionABSTRACT
In order to explore the nature of glucose-6-phosphate dehydrogenase (G6PD) deficiency in south-east Sicily, we have analysed the G6PD gene in 25 unrelated males with abnormal G6PD activity and/or electrophoretic mobility, by using the analysis of the appropriate PCR-amplified fragment of DNA and subsequent digestion by appropriate restriction-enzymes, looking for the presence of certain known G6PD mutations. We amplified the entire G6PD coding sequence into eight fragments, followed by single-strand conformation polymorphism (SSCP) analysis and sequencing of those individual fragments that were found to be abnormal by SSCP. Through these methods we found a total of twelve G6PD Mediterranean variants with the association of a silent mutation 1311 (also known as polymorphic site Bcl I), one G6PD Mediterranean without this association, four G6PD A-Val 68 and two G6PD Santamaria and five G6PD Chatham. In a subject with normal activity a mutation was found in exon 5, designated as G6PD Sao Borja. This is the first report on the molecular analysis of G6PD mutations in Sicily and we have obtained evidence for four distinct classes of variants.
Subject(s)
Genetic Heterogeneity , Glucosephosphate Dehydrogenase Deficiency/genetics , Female , Glucosephosphate Dehydrogenase Deficiency/epidemiology , Humans , Male , Point Mutation , Polymerase Chain Reaction , Sequence Analysis, DNA , Sicily/epidemiologyABSTRACT
Sicily, at the center of the Mediterranean, has been the meeting place of Eastern and Western civilizations, and in the Sicilian population the presence of many different alterations in the globin gene clusters can surely be considered testimony of past colonizations. From 1975 to 1994, 100,000 Sicilian subjects were screened by us to monitor the presence of hemoglobin (Hb) structural variants. In this paper we present the data gathered, emphasizing the high incidence (2.5%) of carriers of at least one abnormal Hb, and the great heterogeneity of globin molecular defects on the island. Twenty-six different mutations were identified: the most common occur in the beta-globin gene (beta(S), beta(C), deltabeta(Lepore), beta(G-San José), beta(O-Arab), but also quite frequent is the mutated allele alpha(J-Oxford). The chromosome haplotypes associated with some of them were characterized. Two uncommon Hbs, Copenhagen and D Punjab, and some 18 rare variants complete the wide spectrum of structural alterations of globin genes in Sicily. We think they are de novo mutations prevalently. It is not possible to exclude that the presence of a few of them is related to migratory phenomena, particularly from North Africa and East Asia. Numerous thalassemic alleles complete the picture of globin gene mutations in Silicy.