ABSTRACT
OBJECTIVE: The racial disparities among patients with endometrial carcinoma have been previously reported. The objective of this study is to analyze and compare the molecular profiles in endometrial cancer in Caucasian and African American patients using a number of known molecular markers. MATERIALS AND METHODS: 147 patients diagnosed with endometrial cancer between 1995 and 2001 were included in the study. Patients' demographics, clinical and pathological data were reviewed. Immunohistochemical staining for p53, VEGF, Ki-67 and HIF-1alpha was performed on tissue micro array sections. Tumors' expression of p53, VEGF, Ki-67, and HIF-1alpha was compared based on ethnicity and tumor type (Type I = endometrioid carcinomas and Type II = non-endometrioid carcinomas). Spearman's correlation and Fisher's Exact Tests were used for statistical analysis and Kaplan-Meier, log-rank and Cox regression were used for survival analysis. RESULTS: 97 patients were Caucasian and 50 patients were African American. The mean age was 62 (33-91) years for Caucasian patients and 63.5 (24-89) years for the African American patients. African American patients had more Type II carcinoma than Caucasian patients (P = 0.055). High p53 expression was statistically significant among the African American patients (49% vs. 30%, P = 0.035) versus Caucasian patients. There was no significant difference demonstrated when comparing the VEGF, Ki-67, and HIF-1alpha expression between the racial groups. Survival analysis showed a trend toward a shorter survival in the African American patients compared to the Caucasian patients; median survival 62 versus 77 months (P = 0.061). On the other hand, we did not find a significant difference in survival by ethnicity when we adjusted for tumor histology. CONCLUSION: While African American patients with endometrial cancer seem to show a trend toward a shorter survival, this seems to be mainly due to the fact that they have a higher proportion of Type II tumors. The molecular profiles for p53, Ki-67, VEGF and HIF-1alpha expression of histologically matched tumors were similar between the two ethnic groups.
Subject(s)
Black or African American , Endometrial Neoplasms/ethnology , White People , Adult , Aged , Aged, 80 and over , Biomarkers, Tumor/analysis , Carcinoma, Endometrioid , Endometrial Neoplasms/genetics , Endometrial Neoplasms/therapy , Female , Humans , Hypoxia-Inducible Factor 1, alpha Subunit , Immunohistochemistry , Ki-67 Antigen/analysis , Middle Aged , Retrospective Studies , Survival Analysis , Treatment Outcome , Tumor Suppressor Protein p53 , Vascular Endothelial Growth Factor A/analysisABSTRACT
INTRODUCTION: The goal of this study is to evaluate the relation of maspin expression and its cellular localization to markers of angiogenesis in epithelial ovarian serous carcinoma (OSC). MATERIALS AND METHODS: We identified 118 patients with high-grade advanced stage OSC who were treated at our institution. Clinical data were collected, and immunohistochemistry (IHC) with antibodies to VEGF, CD34, COX-2, and maspin was performed on paraffin-embedded tumor blocks. CD34 immunostaining was used to determine microvessel density. The correlation between the various molecular markers was assessed using the Chi-square test. Survival analysis was computed using the Kaplan-Meier model, and various prognostic variables were compared using Cox regression analysis. RESULTS: Maspin expression was noted in 81.4% (96/118) of tumors. Expression was localized to the nuclear compartment in 21.2% of cases, whereas 60.2% of cases showed evidence of cytoplasmic +/- nuclear expression. Tumors that exhibited nuclear maspin expression had lower VEGF and COX-2 expression than tumors with negative or cytoplasmic expression. Tumors with high nuclear maspin expression had lower mean MVD than those with low or negative expression. The median survival based on localization of maspin was 1146 days for those with negative tumors, 1803 days for those with nuclear maspin, and 637 days for those with cytoplasmic maspin (P < 0.001). In a Cox regression analysis, maspin localization was an independent prognostic factor. CONCLUSION: Maspin expression and localization seem to play a role in ovarian cancer angiogenesis and progression. High nuclear expression was associated with reduced markers of angiogenesis and prolonged survival.
Subject(s)
Cystadenocarcinoma, Serous/blood supply , Cystadenocarcinoma, Serous/metabolism , Ovarian Neoplasms/blood supply , Ovarian Neoplasms/metabolism , Serpins/biosynthesis , Adult , Aged , Aged, 80 and over , Cyclooxygenase 2/biosynthesis , Cystadenocarcinoma, Serous/pathology , Female , Genes, Tumor Suppressor , Humans , Middle Aged , Neovascularization, Pathologic/metabolism , Ovarian Neoplasms/pathology , Prognosis , Vascular Endothelial Growth Factor A/biosynthesisABSTRACT
Posterior occipital and parietal lobe infarcts shown by computed tomography (CT) scan and magnetic resonance imaging have been associated with eclampsia. Gray-white matter, infarct-like lesions of the right basal ganglia, right posterior parietal, and left posterior parieto-occipital lobes were found by CT scan in a patient at 26 weeks gestation with severe preeclampsia and neurologic deficits. A magnetic resonance image taken 3 days postpartum had similar abnormalities, despite total resolution of the patient's symptomatology. A repeat CT scan performed 6 weeks postpartum showed complete resolution of the multiple infarctions. This is a unique case report that describes these severe brain-imaging findings in a patient with severe preeclampsia and neurologic deficits.
