ABSTRACT
Four case reports of mesenchymal neoplasms showing chromosomal abnormalities are presented. In a case of hemangiopericytoma trisomy 2 and centric fusion 19;21 were present. In a mastocytoma a deleted chromosome 35 was seen. A homogeneously staining region (HSR) on chromosome 1 was detected in a histiocytoma. Trisomy 5 and monosomy 31 were observed in a case of granulocytic sarcoma (chloroma). The lack of mutations in exons 1 and 2 of oncogenes N-ras, K-ras, and H-ras and exons 5, 6, 7, and 8 of tumor suppressor gene p53 in these four patients and in a larger series of investigated dogs (25 hemangiopericytomas, 12 mastocytomas, and 8 histiocytomas) is highlighted.
Subject(s)
Dog Diseases/genetics , Hemangiopericytoma/veterinary , Histiocytoma, Benign Fibrous/veterinary , Leukemia, Myeloid/veterinary , Mast-Cell Sarcoma/veterinary , Tumor Suppressor Protein p53/genetics , ras Proteins/genetics , Animals , Chromosome Aberrations/veterinary , Chromosome Disorders , DNA Mutational Analysis/veterinary , Dogs , Female , Hemangiopericytoma/genetics , Histiocytoma, Benign Fibrous/genetics , Karyotyping/veterinary , Leukemia, Myeloid/genetics , Male , Mast-Cell Sarcoma/genetics , TrisomyABSTRACT
Short term cultures of four feline fibrosarcomas were analysed cytogenetically. There was marked genetic heterogeneity between the four cats, each showing a different clonal abnormality. The aberrations detected were one deleted B2, one marker F1 and two reciprocal translocations, t (A2q; E3q) and t (A1q; B4p).
Subject(s)
Cat Diseases , Chromosome Mapping , Fibrosarcoma/veterinary , Karyotyping , Animals , Cats , Chromosome Aberrations , Chromosome Banding , Chromosome Deletion , Female , Fibrosarcoma/genetics , Fibrosarcoma/pathology , Male , Translocation, Genetic , Tumor Cells, CulturedABSTRACT
Four solid tumours (one haemangiopericytoma, one haemangioendothelioma, one spindle-cell sarcoma and one mammary carcinoma) in dogs were analysed cytogenetically. In the haemangiopericytoma, an additional small chromosomal segment was present. Very complex changes including centric fusions and symmetric meta-centrics 1, 6, 10 and 12 were conspicuous in the highly unbalanced karyotype of the haemangioendothelioma. Complex changes, particularly many centric fusions and a tandem translocation 4/14, were features of the spindle-cell sarcoma. One centric fusion and a symmetric metacentric 13 were present in the mammary carcinoma.
Subject(s)
Dog Diseases/genetics , Karyotyping/veterinary , Neoplasms/veterinary , Adenocarcinoma, Papillary/genetics , Adenocarcinoma, Papillary/veterinary , Animals , Dogs , Hemangioendothelioma/genetics , Hemangioendothelioma/veterinary , Hemangiopericytoma/genetics , Hemangiopericytoma/veterinary , Mammary Neoplasms, Animal/genetics , Neoplasms/genetics , Sarcoma/genetics , Sarcoma/veterinaryABSTRACT
An 11-year-old female cat and a 12-year-old male cat developed fibrosarcomas. Trisomy D1 was observed in a high percentage (20%) of the tumour cells of the female patient while a large marker chromosome F1 was present in 25% of the tumour cells of the male patient.
Subject(s)
Cat Diseases/genetics , Fibrosarcoma/veterinary , Skin Neoplasms/veterinary , Trisomy , Animals , Cats , Female , Fibrosarcoma/genetics , Genetic Markers , Karyotyping , Male , Metaphase , Skin Neoplasms/geneticsABSTRACT
Exon 8 of tumour suppressor gene p53 was sequenced in domestic dogs. Ten nucleotide differences were revealed in a comparison with the feline sequence. A mutation ACT-->TCT (threonine-->serine) in codon 284 was detected in a papilloma of the oral mucosa.
Subject(s)
Dog Diseases/genetics , Exons/genetics , Genes, p53/genetics , Mouth Neoplasms/veterinary , Papilloma/veterinary , Animals , Base Sequence , DNA Primers/chemistry , DNA, Neoplasm/chemistry , Dogs , Molecular Sequence Data , Mouth Neoplasms/genetics , Mutation , Papilloma/geneticsABSTRACT
Exon 8 of tumour suppressor gene p53 was sequenced in domestic cats and showed remarkable similarity to the human sequence. Only four of the 13 nucleotide differences gave rise to interspecific amino acid differences. In an investigated lymphosarcoma we detected a mutation cgg --> tgg (arginine --> tryptophan) in codon no. 282.
Subject(s)
Cat Diseases/genetics , Exons , Genes, p53/genetics , Lymphoma, Non-Hodgkin/veterinary , Mutation/genetics , Amino Acid Sequence , Animals , Base Sequence , Cat Diseases/pathology , Cats , Codon , Humans , Lymphoma, Non-Hodgkin/genetics , Lymphoma, Non-Hodgkin/pathology , Molecular Sequence Data , Polymerase Chain Reaction/veterinary , Sequence Homology, Amino AcidSubject(s)
Adenocarcinoma/veterinary , Chromosome Aberrations/genetics , Dog Diseases/genetics , Mammary Neoplasms, Animal/genetics , Trisomy , Adenocarcinoma/genetics , Adenocarcinoma/pathology , Animals , Chromosome Disorders , Dog Diseases/pathology , Dogs , Female , Karyotyping/veterinary , Mammary Neoplasms, Animal/pathologyABSTRACT
Cells of mammary sarcomas in three dogs were analysed cytogenetically. In an osteoidsarcoma, hyperdiploidy with a range of 92 to 98 chromosomes, and several structural aberrations (for example, a derivative chromosome 1, isochromosome 13 and several bi-armed markers) were observed. Isochromosome 13 was also present in a case of an osteoidchondrosarcoma. In a case of chondro-osteosarcoma both hypodiploidy with a chromosome range of 60 to 66 and hyperdiploidy with a range of 115 to 128 and several centric fusions were observed.
Subject(s)
Chromosome Aberrations/genetics , Dog Diseases/genetics , Mammary Neoplasms, Animal/genetics , Osteosarcoma/veterinary , Animals , Dog Diseases/pathology , Dogs , Female , Karyotyping/veterinary , Mammary Neoplasms, Animal/pathology , Osteosarcoma/genetics , Osteosarcoma/pathologyABSTRACT
The permanent bovine cell line, Madin-Darby bovine kidney, was cytogenetically analyzed. Chromosomal staining indicated 52 metaphase chromosomes with variations from 49 to 54. The isochromosomes 5, 12, and 13, and centric fusions between chromosomes 1 and 26, 9 and 11, 9 and 24, 17 and 25, 18 and 23, 20 and 24, and 24 and 27 were considered marker chromosomes.
Subject(s)
Cattle/genetics , Chromosomes , Fibroblasts/cytology , Genetic Markers , Kidney/cytology , Animals , Cell Line , Chromosome Banding/veterinary , Fibroblasts/ultrastructure , Karyotyping/veterinary , Male , MetaphaseABSTRACT
In a 13-year-old, male rough-haired Dachshund with a malignant melanoma, cytogenetic evaluation of tumour cells showed hyperdiploidy (79 to 81 chromosomes) in 50 per cent of the metaphases. Several bi-armed chromosomes (centric fusions, one isochromosome and two unidentified markers) were observed.
Subject(s)
Dog Diseases/genetics , Melanoma/veterinary , Skin Neoplasms/veterinary , Animals , Diploidy , Dogs , Karyotyping/veterinary , Male , Melanoma/geneticsABSTRACT
Two female (12 and 14 years old) and one male dog (10 years old) developed haemangiopericytomas. Three copies of chromosome 2 were present in the tumours of all three dogs. Additional alterations were an interstitially deleted chromosome 1, centric fusions 3/34 and 8/15, isochromosome 32 and two unidentified marker chromosomes (M1, M2) in one bitch. In the other bitch, the additional alterations were trisomy 37, centric fusions 8/38 and 30/31, and a derivative chromosome 13 (der13). In the male dog, tandem fusion 2/19 was present in addition to two structurally normal chromosomes 2.
Subject(s)
Dog Diseases/genetics , Hemangiopericytoma/veterinary , Mammary Neoplasms, Animal/genetics , Soft Tissue Neoplasms/veterinary , Trisomy , Animals , Chromosome Banding/veterinary , Dogs , Female , Hemangiopericytoma/genetics , Hindlimb , Male , Soft Tissue Neoplasms/geneticsABSTRACT
An 8-year-old German Shepherd bitch developed a haemangioendothelioma in the mammary region. The cytogenetic characterization of the tumour cells revealed a chromosome number of 80 plus a double minute. Several aberrations were detected: trisomies 4, 35, and 38, a centric fusion 8/22 and a tandem translocation 9/23.
Subject(s)
Chromosome Aberrations , Dog Diseases/pathology , Hemangioendothelioma/veterinary , Mammary Neoplasms, Animal/pathology , Animals , Dogs , Female , Hemangioendothelioma/pathology , Karyotyping , MetaphaseABSTRACT
Two dogs developed osteosarcomas. In both cases, the cytogenetic analysis revealed the presence of numerically and structurally altered karyotypes. The chromosome ranges were 90 to 99 and 65 to 67, respectively. Centric fusions frequently identified were 1/3, 1/30 and 2/9 in one case, 3/19, 12/18 and 13/17 in the other.
Subject(s)
Bone Neoplasms/veterinary , Dog Diseases/pathology , Femoral Neoplasms/veterinary , Osteosarcoma/veterinary , Tibia/pathology , Animals , Bone Neoplasms/genetics , Bone Neoplasms/pathology , Dog Diseases/genetics , Dogs , Female , Femoral Neoplasms/genetics , Femoral Neoplasms/pathology , Karyotyping , Osteosarcoma/genetics , Osteosarcoma/pathology , Tumor Cells, CulturedABSTRACT
An eight-year-old male cat developed a sarcoma. The cytogenetic evaluation of the tumour cells showed the presence of hyperdiploidy (range 40 to 46 chromosomes). This hyperdiploidy was encountered in all the cells examined. Extra-chromosomes numbers C1, C2, B4, D4 and E3 were mainly responsible for the hyperdiploid chromosomal complements. There was a high incidence of monosomy E3.
Subject(s)
Cat Diseases/pathology , Chromosome Aberrations/veterinary , Diploidy , Sarcoma/veterinary , Skin Neoplasms/veterinary , Animals , Cats , Chromosome Aberrations/pathology , Chromosome Banding , Chromosome Disorders , Male , Metaphase , Sarcoma/pathology , Skin Neoplasms/pathologyABSTRACT
A 14-year-old German shepherd dog developed an alveolar adenoma of the thyroid. The cytogenetic evaluation of the tumour cells showed two ranges of chromosome numbers. Centric fusions 2/32, 5/26, 6/11, 8/11, 9/23 and 14/31 were G-band identified in this complex change of karyotype.
Subject(s)
Adenoma/veterinary , Dog Diseases/genetics , Thyroid Neoplasms/veterinary , Adenoma/genetics , Adenoma/pathology , Animals , Chromosome Banding , Dog Diseases/pathology , Dogs , Karyotyping/veterinary , Male , Metaphase , Thyroid Neoplasms/genetics , Thyroid Neoplasms/pathologyABSTRACT
Tumor cells of two cases of mammary neoplasms in dog were investigated cytogenetically. An 11-year-old cocker spaniel showed a marked X-chromosome in most metaphases (80%). In a 12-year-old poodle, tumor cells with the following abnormalities were found: trisomy 1, monosomy X and two marker chromosomes.
Subject(s)
Adenocarcinoma/veterinary , Dog Diseases/genetics , Mammary Neoplasms, Animal/genetics , Osteosarcoma/veterinary , X Chromosome , Adenocarcinoma/genetics , Animals , Chromosome Banding , Dogs , Female , Karyotyping/veterinary , Osteosarcoma/genetics , Sex Chromosome Aberrations/genetics , Sex Chromosome Aberrations/veterinaryABSTRACT
The karyotypes of three avian species--Meleagris gallopavo, Anser anser L., and Columba livia domestica--were investigated by means of counterstain-enhanced fluorescence techniques (chromomycin A3/distamycin A/DAPI followed by DAPI/actinomycin D staining). A heterochromatin characterization of macro- and microchromosomes was performed. CMA3-positive (GC-rich) regions in the turkey included the telomeres of chromosomes 1, 3, 4, and Z. In the goose, the chromosome 2 was also CMA3-bright at the telomeres. The W chromosome possessed large amounts of CMA3-bright material on the short arm in both the turkey and the goose. Two types of centromeric heterochromatin were distinguished on acro- to telocentric chromosomes 6 to 14 in the pigeon. The microchromosomal heterochromation of the turkey and goose was GC-rich but had a high degree of variation. In the pigeon, several microchromosomes possessed predominantly AT-rich heterochromatin.
Subject(s)
Columbidae/genetics , Geese/genetics , Heterochromatin , Turkeys/genetics , Animals , Base Composition , Cells, Cultured , Chromosome Banding , Female , Fibroblasts , Fluorescent Dyes , Heterochromatin/chemistry , Karyotyping , MaleABSTRACT
An 11-year-old German shepherd bitch developed a neurofibroma in the right shank. On cytogenetic analysis of the tumour cells 90% presented themselves with trisomy 2, a derivative chromosome 13 (der13) and centric fusions 10/35 and 24/31.
Subject(s)
Chromosome Aberrations , Dog Diseases/genetics , Neurofibroma/veterinary , Animals , Dogs , Female , Karyotyping , Metaphase , Neurofibroma/genetics , TrisomyABSTRACT
In a 9-year-old female dog (Basset Artesian Normand) with a mammary adenocarcinoma, cytogenetic evaluation of tumor cells showed a chromosome number of 76 in most metaphases (95%). The following abnormalities were found: symmetric metacentric chromosomes 1 and 6, centric fusion 3/38, a marker X-chromosome (Xmar) and a biarmed small marker chromosome (mar). In the remaining metaphases (5%) there were additional biarmed marker chromosomes present.
Subject(s)
Adenocarcinoma/veterinary , Chromosome Aberrations , Dog Diseases/genetics , Mammary Neoplasms, Animal/genetics , Adenocarcinoma/genetics , Adenocarcinoma/pathology , Animals , Dog Diseases/pathology , Dogs , Female , Karyotyping/veterinary , Mammary Neoplasms, Animal/pathologyABSTRACT
A 15-year-old dachshund bitch developed a haemangiopericytoma in the perineal region. The cytogenetic evaluation of the tumour cells showed a chromosome number of 74. The following abnormalities were found: an intersitially deleted chromosome no. 1 and centric fusions 5/6, 5/14, 7/15 and 9/17.
