ABSTRACT
Ovaries are highly complex organs displaying morphological, molecular and functional differences between their cortical zona parenchymatosa and medullary zona vasculosa, and also between the different cyclic luteal stages. Objective of the present study was to validate expression stability of twelve putative reference genes (RGs) in bovine ovaries, considering the intrinsic heterogeneity of bovine ovarian tissue with regard to different luteal stages and intra-ovarian localizations. The focus was on identifying RGs, which are suitable to normalize RT-qPCR results of ovaries collected from clinical healthy cattle, irrespective of localization and the hormonal stage. Expression profiles of twelve potential reference genes (GAPDH, ACTB, YWHAZ, HPRT1, SDHA, UBA52, POLR2C, RPS9, ACTG2, H3F3B, RPS18 and RPL19) were analysed. Evaluation of gene expression differences was performed using genorm, normfinder, and bestkeeper software. The most stably expressed genes according to genorm, normfinder and bestkeeper approaches contained the candidates H3F3B, RPS9, YWHAZ, RPS18, POLR2C and UBA52. Of this group, the genes YWHAZ, H3F3B and RPS9 could be recommended as best-suited RGs for normalization purposes on healthy bovine ovaries irrespective of the luteal stage or intra-ovarian localization.
Subject(s)
Cattle/genetics , Gene Expression Regulation/genetics , Ovary/physiology , Ribosomal Proteins/genetics , Abattoirs , Algorithms , Animals , Cattle/anatomy & histology , Corpus Luteum/physiology , DNA, Complementary/metabolism , Estrous Cycle/genetics , Female , Ovary/anatomy & histology , Pregnancy , RNA/isolation & purification , Reference Standards , SoftwareABSTRACT
The neutron index of refraction is generally derived theoretically in the Fermi approximation. However, the Fermi approximation neglects the effects of the binding of the nuclei of a material as well as multiple scattering. Calculations by Nowak introduced correction terms to the neutron index of refraction that are quadratic in the scattering length and of order 10(-3) fm for hydrogen and deuterium. These correction terms produce a small shift in the final value for the coherent scattering length of H2 in a recent neutron interferometry experiment.
ABSTRACT
We have performed the first high precision measurement of the coherent neutron scattering length of deuterium in a pure sample using neutron interferometry. We find b(nd)=(6.665+/-0.004) fm in agreement with the world average of previous measurements using different techniques, b(nd)=(6.6730+/-0.0045) fm. We compare the new world average for the nd coherent scattering length b(nd)=(6.669+/-0.003) fm to calculations of the doublet and quartet scattering lengths from several modern nucleon-nucleon potential models with three-nucleon force (3NF) additions and show that almost all theories are in serious disagreement with experiment. This comparison is a more stringent test of the models than past comparisons with the less precisely determined doublet scattering length of (2)a(nd)=(0.65+/-0.04) fm.
ABSTRACT
AIDS: Dealing with an AIDS-related death in the workplace is always difficult, but even more so in organizations that deal with AIDS issues every day. It is helpful for all employees, including supervisors, to acknowledge their feelings of loss and sadness. Some may experience burnout from the stress of dealing with multiple losses. Programs have been developed to help co-workers and supervisors deal with loss. Some organizations set aside time for employees to practice their own form of support.^ieng
