Subject(s)
AIDS-Related Complex/therapy , Acquired Immunodeficiency Syndrome/therapy , AIDS-Related Complex/immunology , Acquired Immunodeficiency Syndrome/immunology , Adjuvants, Immunologic/therapeutic use , Humans , Hypersensitivity, Delayed , Immunotherapy , Lymphokines/therapeutic use , T-Lymphocytes, Helper-Inducer/drug effects , T-Lymphocytes, Helper-Inducer/immunologyABSTRACT
Flow cytometry, a technique which allows rapid quantitation of the percentages of a cell population in the various cell cycle phases, closely duplicated the previously reported circadian rhythm for mouse bone marrow DNA synthesis. The effect of ara-C, an S phase specific anti-neoplastic drug, on this rhythm was investigated. Ara-C was shown to suppress this rhythm to a much greater degree when injected at the peak of the bone marrow DNA synthesis as compared to the low. Thus, damage to the bone marrow during treatment with ara-C may be reduced if the circadian rhythm of bone marrow DNA synthesis is taken into account.
Subject(s)
Bone Marrow/drug effects , Circadian Rhythm/drug effects , Cytarabine/pharmacology , DNA/biosynthesis , Animals , Bone Marrow/metabolism , Female , Flow Cytometry , MuridaeABSTRACT
Mouse L1210 leukemia cells killed by ara-C, an anti-tumor agent widely used against leukemia, were demonstrated to be in the S phase of the cell cycle by flow microfluorometry. The point in S phase at which the L1210 cells died was shown to be dose dependent. At higher concentrations of ara-C cells died earlier in the S phase.
Subject(s)
Cell Cycle/drug effects , Cytarabine/pharmacology , Animals , Cell Survival/drug effects , DNA, Neoplasm/metabolism , Fluorometry/methods , Leukemia L1210/metabolism , MiceSubject(s)
Plant Cells , Staining and Labeling , Trees , Cell Nucleolus , Cell Nucleus , Cytoplasm , Indicators and Reagents , Methods , Microscopy , Tannins/biosynthesis , Vaginal SmearsSubject(s)
Extraterrestrial Environment , Plant Development , Chloroplasts/cytology , Culture Media , Culture Techniques , Cytoplasm , Endoplasmic Reticulum , Golgi Apparatus , Microscopy, Electron , Mitochondria , Nitrogen/analysis , Oryza/growth & development , Oxygen Consumption , Phosphorus/analysis , Plant Cells , Plants/analysis , Plants/metabolism , Plants, Toxic , Nicotiana/growth & development , Trees/growth & development , Zea mays/growth & developmentSubject(s)
Liver Neoplasms/enzymology , Liver/enzymology , Lyases/isolation & purification , Lymphoma, Non-Hodgkin/enzymology , Aldehydes , Animals , Chromatography, DEAE-Cellulose , Chromatography, Gel , Glutathione , Guanidines/pharmacology , Kinetics , Liver/drug effects , Mice , Mice, Inbred DBA , Molecular Weight , Sarcoma, Experimental/enzymologySubject(s)
Amino Acids/analysis , Chromatography, Gas , Acylation , Fluoroacetates/analysis , Hydantoins/analysis , Methods , Sulfides/analysisSubject(s)
Lymphoma, Non-Hodgkin/metabolism , Nucleic Acids/metabolism , Phosphorus/metabolism , Skin/metabolism , Animals , Lymphoma, Non-Hodgkin/chemically induced , Methylcholanthrene , Mice , Neoplasms, Experimental/chemically induced , Neoplasms, Experimental/metabolism , Papilloma/chemically induced , Skin Neoplasms/chemically inducedABSTRACT
Cultures of Anabaena cylindrica Lemm. and Eucapsis sp. were harvested, freeze-dried, and prepared for electron microscope studies. A comparison of the freeze-dried preparations was made with control specimens not freeze-dried. Fixation and embedding were according to the Ryter and Kellenberger technique. The photosynthelic thylakoids of the freeze-dried cells were more distinct and less distorted than those of the control cells. Freeze-dried Eucapsis sp. cells exhibited a prominent mucous capsule, whereas in the nonfreeze-dried, no such capsule could be discerned.