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1.
Bioelectromagnetics ; 41(4): 308-317, 2020 May.
Article in English | MEDLINE | ID: mdl-32043629

ABSTRACT

The expected imminent widespread use of electromobility in transport systems draws attention to the possible effects of human exposure to magnetic fields generated inside electric vehicles and during their recharge. The current trend is to increase the capacity of the battery inside the vehicles to extend the available driving range and to increase the power of recharging columns to reduce the time required for a full recharge. This leads to higher currents and potentially stronger magnetic fields. The Interoperability Center of the Joint Research Center started an experimental activity focused on the assessment of low-frequency magnetic fields emitted by five fast-charging devices available on the market in recharge and standby conditions. The aim of this study was to contribute to the development of a standard measurement procedure for the assessment of magnetic fields emitted by direct current charging columns. The spectrum and amplitudes of the magnetic field, as well as exposure indices according to guidelines for the general public and occupational exposure, were recorded by means of a magnetic field probe analyzer. The worst-case scenario for instantaneous physical direct and indirect effects was identified. Measurements within the frequency range of 25 Hz-2 kHz revealed localized magnetic flux density peaks above 100 µT at the 50 Hz frequency in three out of five chargers, registered in close proximity during the recharge. Beyond this distance, exposure indices were recorded showing values below 50% of reference levels. Bioelectromagnetics. 2020;41:308-317 © 2020 The Authors. Bioelectromagnetics published by Wiley Periodicals, Inc.


Subject(s)
Electric Power Supplies/adverse effects , Electromagnetic Fields , Automobiles
2.
Transp Res D Transp Environ ; 41: 348-366, 2015 Dec.
Article in English | MEDLINE | ID: mdl-32288595

ABSTRACT

Electrification is widely considered as a viable strategy for reducing the oil dependency and environmental impacts of road transportation. In pursuit of this strategy, most attention has been paid to electric cars. However, substantial, yet untapped, potentials could be realized in urban areas through the large-scale introduction of electric two-wheelers. Here, we review the environmental, economic, and social performance of electric two-wheelers, demonstrating that these are generally more energy efficient and less polluting than conventionally-powered motor vehicles. Electric two-wheelers tend to decrease exposure to pollution as their environmental impacts largely result from vehicle production and electricity generation outside of urban areas. Our analysis suggests that the price of e-bikes has been decreasing at a learning rate of 8%. Despite price differentials of 5000 ± 1800 EUR2012 kW h-1 in Europe, e-bikes are penetrating the market because they appear to offer an apparent additional use value relative to bicycles. Mid-size and large electric two-wheelers do not offer such an additional use value compared to their conventional counterparts and constitute niche products at price differentials of 700 ± 360 EUR2012 kW-1 and 160 ± 90 EUR2012 kW-1, respectively. The large-scale adoption of electric two-wheelers can reduce traffic noise and road congestion but may necessitate adaptations of urban infrastructure and safety regulations. A case-specific assessment as part of an integrated urban mobility planning that accounts, e.g., for the local electricity mix, infrastructure characteristics, and mode-shift behavior, should be conducted before drawing conclusions about the sustainability impacts of electric two-wheelers.

3.
J Biol Chem ; 281(1): 491-500, 2006 Jan 06.
Article in English | MEDLINE | ID: mdl-16267052

ABSTRACT

Storage and degradation of triglycerides are essential processes to ensure energy homeostasis and availability of precursors for membrane lipid synthesis. Recent evidence suggests that an emerging class of enzymes containing a conserved patatin domain are centrally important players in lipid degradation. Here we describe the identification and characterization of a major triglyceride lipase of the adipose triglyceride lipase/Brummer family, Tgl4, in the yeast Saccharomyces cerevisiae. Elimination of Tgl4 in a tgl3 background led to fat yeast, rendering growing cells unable to degrade triglycerides. Tgl4 and Tgl3 lipases localized to lipid droplets, independent of each other. Serine 315 in the GXSXG lipase active site consensus sequence of the patatin domain of Tgl4 is essential for catalytic activity. Mouse adipose triglyceride lipase (which also contains a patatin domain but is otherwise highly divergent in primary structure from any yeast protein) localized to lipid droplets when expressed in yeast, and significantly restored triglyceride breakdown in tgl4 mutants in vivo. Our data identify yeast Tgl4 as a functional ortholog of mammalian adipose triglyceride lipase.


Subject(s)
Lipase/genetics , Lipase/metabolism , Lipolysis/physiology , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Triglycerides/metabolism , Amino Acid Sequence , Animals , Conserved Sequence , Diglycerides/metabolism , Evolution, Molecular , Kinetics , Lipase/chemistry , Mammals , Mice , Molecular Sequence Data , Mutation , Obesity/enzymology , Protein Structure, Tertiary , Saccharomyces cerevisiae Proteins/chemistry , Substrate Specificity
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