Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration.

The flow focusing is a fundamental prior step in order to sort, analyze, and detect particles or cells. The standard hydrodynamic approach requires two fluids to be injected into the microfluidic device: one containing the sample and the other one, called the sheath fluid, allows squeezing the sample fluid into a narrow stream. The major drawback of this approach is the high complexity of the layout for microfluidic devices when parallel streams are required. In this work, we present a novel parallelized microfluidic device that enables hydrodynamic focusing in each microchannel using a single feed flow. At each of the parallel channels, a cross-filter region is present that allows removing fluid from the sample fluid. This fluid is used to create local sheath fluids that hydrodynamically pinch the sample fluid. The great advantage of the proposed device is that, since only one inlet is needed, multiple parallel micro-channels can be easily introduced into the design. In the paper, the design method is described and the numerical simulations performed to define the optimal design are summarized. Moreover, the operational functionality of devices tested by using both polystyrene beads and Acute Lymphoid Leukemia cells are shown.

Co- and cross-flow extensions in an elliptical optical trap.

The extension of a particle (translational deviation) in an isotropic harmonic potential well is linearly proportional and parallel to the applied force. In an anisotropic trapping potential, the extension is instead related to the applied force by a compliance tensor. Using the focal spot of a high numerical aperture zone plate to create an elliptical potential and microfluidics to apply a calibrated force, we measure the two-dimensional extension of a trapped spherical particle. As a function of the orientation of the elliptical potential, the extension sweeps out a circular trajectory, exhibiting extensions both parallel (coflow) and perpendicular (cross flow) to the direction of the flow. The results fit well to a compliance tensor model.

Spring constant modulation in a zone plate tweezer using linear polarization.

At large NAs a micro-Fresnel zone plate produces a focal spot that is more elliptical than that produced by an objective lens with the same NA. Using this anisotropy we demonstrate a method for modulating the spring constant of an optical trap by rotating the linear input polarization. The focal spot ellipticity is enhanced by the apodization factor of the zone plate and its extremely high NA. By measuring the positions of trapped particles we obtain two-dimensional histograms of particle position. These indicate that the trap spring constant is 2.75 times larger perpendicular to the incident polarization than along it. The elliptical focal spot distribution can be rotated by rotating the incident polarization, allowing the spring constant along a given direction to be modulated.

Polarization beam splitter based on a photonic crystal heterostructure.

The design and characterization of a photonic crystal (PC) polarization beam splitter (PBS) that operates with an extinction ratio of greater than 15 dB for both polarizations are presented. The PBS is fabricated on a silicon-on-insulator (SOI) wafer where the input and output ports consist of 5 mum wide ridge waveguides. A large spectral shift is observed in the dispersion plots of the lowest-order even (TE-like) and odd (TM-like) modes due to the SOI confinement. Because of this shift, the TE-like mode is close to a directional gap at the top of the band, and the TM-like mode is in a low-frequency regime where the dispersion surface is almost isotropic. We show that the TE-like mode has very high reflection at the interface between the two PCs, whereas the TM-like mode exhibits a very high transmission.