Subject(s)
Carcinoma, Large Cell/virology , Carcinoma, Squamous Cell/virology , DNA, Viral/analysis , Lung Neoplasms/virology , Papillomaviridae/genetics , Papillomaviridae/isolation & purification , Polymerase Chain Reaction , Adenocarcinoma/genetics , Adenocarcinoma/virology , Adult , Carcinoid Tumor/genetics , Carcinoid Tumor/virology , Carcinoma, Adenosquamous/genetics , Carcinoma, Adenosquamous/virology , Carcinoma, Large Cell/genetics , Carcinoma, Squamous Cell/genetics , Chromosomes, Human, Pair 17/genetics , DNA, Viral/genetics , Exons/genetics , Female , Genes, p53/genetics , Humans , Lung Neoplasms/genetics , Mutation/genetics , Polymorphism, Single-Stranded ConformationalABSTRACT
Oropharyngeal squamous cell cancers (SCCs) were examined for human papillomavirus (HPV) related DNA sequences. The techniques employed were Southern blotting under stringent and non stringent conditions, dot blotting, primer directed gene amplification using the polymerase chain reaction (PCR), and in-situ hybridization. HPV 16 DNA was found in 4 of 30 tumor samples using PCR. HPV 16 DNA was found in 2 further tumors using in-situ hybridization. No HPV DNA could be found by Southern blot or dot blot in any tumor sample. The Southern blot assays were sensitive enough to detect clonally integrated HPV 16 DNA of length greater than 250 bp in the tumors. While HPV DNA is present in some oropharyngeal SCCs, there is no molecular evidence to support a causal association of HPV 16 gene products with continued tumor growth in oropharyngeal cancer.
Subject(s)
Carcinoma, Squamous Cell/microbiology , DNA, Viral/isolation & purification , Oropharyngeal Neoplasms/microbiology , Papillomaviridae/isolation & purification , Tumor Virus Infections/microbiology , Adult , Aged , Base Sequence , Blotting, Southern , DNA Probes, HPV , DNA, Viral/genetics , Female , Humans , Male , Middle Aged , Molecular Sequence Data , Papillomaviridae/genetics , Polymerase Chain Reaction , Sensitivity and SpecificityABSTRACT
Exfoliated cervical epithelial cells from women 6 weeks postpartum were analyzed for human papillomavirus (HPV) DNA using the polymerase chain reaction, and results were compared with those from buccal mucosal smears from their babies. Eleven mothers had genital genotypes of HPV in their cervical smears, and the children of 8 of these had HPV of the same genotype in buccal mucosal cell samples. Nineteen mothers had no HPV DNA detected in their cervical smears, and 1 of the buccal mucosal cell samples from their children was positive for HPV DNA (p < 0.0001). Contamination of a child's mouth with 'genital' HPV from a mother's cervix appears to occur commonly at birth or in the perinatal period, and to persist for at least 6 weeks. This observation has implications for the epidemiology and management of HPV associated cancer and precancerous conditions in the cervix and the mouth.
Subject(s)
Papillomaviridae , Pregnancy Complications, Infectious/microbiology , Tumor Virus Infections/transmission , Cervix Uteri/microbiology , Delivery, Obstetric , Female , Genotype , Humans , Infant , Infant, Newborn , Mouth/microbiology , Papillomaviridae/genetics , Papillomaviridae/isolation & purification , Pregnancy , Tumor Virus Infections/microbiologyABSTRACT
The prevalence of six genital genotypes of HPV was assessed in the clinically normal oral mucosa of an adult Caucasian population, and three methods of sample collection compared. HPV DNA was detected in the mouth of 60% of 60 subjects. HPV 16 was the most prevalent genotype, and positive samples were found most frequently in men over 50. A 3% sucrose mouthwash produced more positive results (51%) than mucosal scrapes of three separate sites (45%) or buccal mucosal biopsies (12%). There was no association of a positive result for HPV DNA with any particular mucosal site. A mouthwash was the preferred single screening method for epidemiologic studies of HPV DNA in the mouth, but the greatest yield of positive samples was obtained if multiple sampling techniques were employed.
