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1.
Curr Biol ; 33(5): 807-816.e4, 2023 03 13.
Article in English | MEDLINE | ID: mdl-36706752

ABSTRACT

Germline mutations upregulating RAS signaling are associated with multiple developmental disorders. A hallmark of these conditions is that the same mutation may present vastly different phenotypes in different individuals, even in monozygotic twins. Here, we demonstrate how the origins of such largely unexplained phenotypic variations may be dissected using highly controlled studies in Drosophila that have been gene edited to carry activating variants of MEK, a core enzyme in the RAS pathway. This allowed us to measure the small but consistent increase in signaling output of such alleles in vivo. The fraction of mutation carriers reaching adulthood was strongly reduced, but most surviving animals had normal RAS-dependent structures. We rationalize these results using a stochastic signaling model and support it by quantifying cell fate specification errors in bilaterally symmetric larval trachea, a RAS-dependent structure that allows us to isolate the effects of mutations from potential contributions of genetic modifiers and environmental differences. We propose that the small increase in signaling output shifts the distribution of phenotypes into a regime, where stochastic variation causes defects in some individuals, but not in others. Our findings shed light on phenotypic heterogeneity of developmental disorders caused by deregulated RAS signaling and offer a framework for investigating causal effects of other pathogenic alleles and mild mutations in general.


Subject(s)
Signal Transduction , ras Proteins , Animals , ras Proteins/genetics , ras Proteins/metabolism , Signal Transduction/genetics , Mutation , Drosophila/genetics , Drosophila/metabolism , Phenotype
2.
Dev Biol ; 490: 100-109, 2022 10.
Article in English | MEDLINE | ID: mdl-35870495

ABSTRACT

Biological tubes serve as conduits through which gas, nutrients and other important fluids are delivered to tissues. Most biological tubes consist of multiple cells connected by epithelial junctions. Unlike these multicellular tubes, seamless tubes are unicellular and lack junctions. Seamless tubes are present in various organ systems, including the vertebrate vasculature, C.elegans excretory system, and Drosophila tracheal system. The Drosophila tracheal system is a network of air-filled tubes that delivers oxygen to all tissues. Specialized cells within the tracheal system, called terminal cells, branch extensively and form seamless tubes. Terminal tracheal tubes are polarized; the lumenal membrane has apical identity whereas the outer membrane exhibits basal characteristics. Although various aspects of membrane trafficking have been implicated in terminal cell morphogenesis, the precise secretory pathway requirements for basal and apical membrane growth have yet to be elucidated. In the present study, we demonstrate that anterograde trafficking, retrograde trafficking and Golgi-to-plasma membrane vesicle fusion are each required for the complex branched architecture of the terminal cell, but their inputs during seamless lumen formation are more varied. The COPII subunit, Sec31, and ER exit site protein, Sec16, are critical for subcellular tube architecture, whereas the SNARE proteins Syntaxin 5, Syntaxin 1 and Syntaxin 18 are more generally required for seamless tube growth and maintenance. These data suggest that distinct components of the secretory pathway have differential contributions to basal and apical membrane growth and maintenance during terminal cell morphogenesis.


Subject(s)
Drosophila Proteins , Drosophila , Animals , Caenorhabditis elegans/metabolism , Drosophila/metabolism , Drosophila Proteins/genetics , Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Morphogenesis , Secretory Pathway , Trachea/metabolism
3.
Dev Biol ; 459(2): 79-86, 2020 03 15.
Article in English | MEDLINE | ID: mdl-31758943

ABSTRACT

Building a left-right (L-R) asymmetric organ requires asymmetric information. This comes from various sources, including asymmetries in embryo-scale genetic cascades (including the left-sided Nodal cascade), organ-intrinsic mechanical forces, and cell-level chirality, but the relative influence of these sources and how they collaborate to drive asymmetric morphogenesis is not understood. During zebrafish heart development, the linear heart tube extends to the left of the midline in a process known as jogging. The jogged heart then undergoes dextral (i.e. rightward) looping to correctly position the heart chambers relative to one another. Left lateralized jogging is governed by the left-sided expression of Nodal in mesoderm tissue, while looping laterality is mainly controlled by heart-intrinsic cell-level asymmetries in the actomyosin cytoskeleton. The purpose of lateralized jogging is not known. Moreover, after jogging, the heart tube returns to an almost midline position and so it is not clear whether or how jogging may impact the dextral loop. Here, we characterize a novel loss-of-function mutant in the zebrafish Nodal homolog southpaw (spaw) that appears to be a true null. We then assess the relationship between jogging and looping laterality in embryos lacking asymmetric Spaw signals. We found that the probability of a dextral loop occurring, does not depend on asymmetric Spaw signals per se, but does depend on the laterality of jogging. Thus, we conclude that the role of leftward jogging is to spatially position the heart tube in a manner that promotes robust dextral looping. When jogging laterality is abnormal, the robustness of dextral looping decreases. This establishes a cooperation between embryo-scale Nodal-dependent L-R asymmetries and organ-intrinsic cellular chirality in the control of asymmetric heart morphogenesis and shows that the transient laterality of the early heart tube has consequences for later heart morphogenetic events.


Subject(s)
Body Patterning/genetics , Embryonic Development/genetics , Heart/embryology , Organogenesis/genetics , Zebrafish/embryology , Animals , Female , Gene Expression Regulation, Developmental , Gene Knockdown Techniques , Loss of Function Mutation , Male , Mesoderm/metabolism , Myocardium/metabolism , Nodal Protein/metabolism , Signal Transduction/genetics , Transforming Growth Factor beta2/genetics , Transforming Growth Factor beta2/metabolism , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
4.
Cell Rep ; 14(8): 1841-9, 2016 Mar 01.
Article in English | MEDLINE | ID: mdl-26904945

ABSTRACT

Cilia are microtubule-based projections that function in the movement of extracellular fluid. This requires cilia to be: (1) motile and driven by dynein complexes and (2) correctly polarized on the surface of cells, which requires planar cell polarity (PCP). Few factors that regulate both processes have been discovered. We reveal that C21orf59/Kurly (Kur), a cytoplasmic protein with some enrichment at the base of cilia, is needed for motility; zebrafish mutants exhibit characteristic developmental abnormalities and dynein arm defects. kur was also required for proper cilia polarization in the zebrafish kidney and the larval skin of Xenopus laevis. CRISPR/Cas9 coupled with homologous recombination to disrupt the endogenous kur locus in Xenopus resulted in the asymmetric localization of the PCP protein Prickle2 being lost in mutant multiciliated cells. Kur also makes interactions with other PCP components, including Disheveled. This supports a model wherein Kur plays a dual role in cilia motility and polarization.


Subject(s)
LIM Domain Proteins/genetics , Microtubules/metabolism , Xenopus laevis/genetics , Zebrafish Proteins/genetics , Zebrafish/genetics , Animals , Binding Sites , CRISPR-Cas Systems , Cell Movement , Cell Polarity , Cilia/metabolism , Dishevelled Proteins/genetics , Dishevelled Proteins/metabolism , Embryo, Nonmammalian , Gene Expression , Genetic Loci , Homologous Recombination , Kidney/cytology , Kidney/growth & development , Kidney/metabolism , LIM Domain Proteins/metabolism , Larva/genetics , Larva/growth & development , Larva/metabolism , Membrane Proteins , Microtubules/ultrastructure , Mutation , Protein Binding , Signal Transduction , Skin/cytology , Skin/growth & development , Skin/metabolism , Xenopus Proteins/genetics , Xenopus Proteins/metabolism , Xenopus laevis/embryology , Xenopus laevis/metabolism , Zebrafish/embryology , Zebrafish/metabolism , Zebrafish Proteins/metabolism
5.
Curr Biol ; 24(15): 1756-64, 2014 Aug 04.
Article in English | MEDLINE | ID: mdl-25065756

ABSTRACT

Most tubes have seams (intercellular or autocellular junctions that seal membranes together into a tube), but "seamless" tubes also exist. In Drosophila, stellate-shaped tracheal terminal cells make seamless tubes, with single branches running through each of dozens of cellular extensions. We find that mutations in braided impair terminal cell branching and cause formation of seamless tube cysts. We show that braided encodes Syntaxin7 and that cysts also form in cells deficient for other genes required either for membrane scission (shibire) or for early endosome formation (Rab5, Vps45, and Rabenosyn-5). These data define a requirement for early endocytosis in shaping seamless tube lumens. Importantly, apical proteins Crumbs and phospho-Moesin accumulate to aberrantly high levels in braided terminal cells. Overexpression of either Crumbs or phosphomimetic Moesin induced lumenal cysts and decreased terminal branching. Conversely, the braided seamless tube cyst phenotype was suppressed by mutations in crumbs or Moesin. Indeed, mutations in Moesin dominantly suppressed seamless tube cyst formation and restored terminal branching. We propose that early endocytosis maintains normal steady-state levels of Crumbs, which recruits apical phosphorylated (active) Moe, which in turn regulates seamless tube shape through modulation of cortical actin filaments.


Subject(s)
Drosophila Proteins/genetics , Drosophila melanogaster/physiology , Endocytosis , Membrane Proteins/genetics , Animals , Drosophila Proteins/metabolism , Drosophila melanogaster/growth & development , Larva/physiology , Membrane Proteins/metabolism , Mutation
6.
Nat Cell Biol ; 15(2): 137-9, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23377027

ABSTRACT

Most organs are composed of tubes of differing cellular architectures, including intracellular 'seamless' tubes. Two studies examining the morphogenesis of the seamless tubes formed by the excretory canal cell in Caenorhabditis elegans reveal a previously unappreciated role for osmoregulation of tubulogenesis: hyperosmotic shock recruits canalicular vesicles to the lumenal membrane to promote seamless tube growth.


Subject(s)
Aquaporins/metabolism , Caenorhabditis elegans Proteins/metabolism , Caenorhabditis elegans/metabolism , Cell Membrane/metabolism , Cytoskeletal Proteins/metabolism , Homeodomain Proteins/metabolism , Lymphangiogenesis , Lymphatic Vessels/metabolism , Tumor Suppressor Proteins/metabolism , Water-Electrolyte Balance , Animals , Humans
7.
Nat Cell Biol ; 14(4): 386-93, 2012 Mar 11.
Article in English | MEDLINE | ID: mdl-22407366

ABSTRACT

Seamless tubes form intracellularly without cell-cell or autocellular junctions. Such tubes have been described across phyla, but remain mysterious despite their simple architecture. In Drosophila, seamless tubes are found within tracheal terminal cells, which have dozens of branched protrusions extending hundreds of micrometres. We find that mutations in multiple components of the dynein motor complex block seamless tube growth, raising the possibility that the lumenal membrane forms through minus-end-directed transport of apical membrane components along microtubules. Growth of seamless tubes is polarized along the proximodistal axis by Rab35 and its apical membrane-localized GAP, Whacked. Strikingly, loss of whacked (or constitutive activation of Rab35) leads to tube overgrowth at terminal cell branch tips, whereas overexpression of Whacked (or dominant-negative Rab35) causes formation of ectopic tubes surrounding the terminal cell nucleus. Thus, vesicle trafficking has key roles in making and shaping seamless tubes.


Subject(s)
Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Dyneins/metabolism , Trachea/growth & development , Trachea/metabolism , rab GTP-Binding Proteins/metabolism , Animals , Trachea/cytology
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