Presence and possible role of vascular endothelial growth factor in thyroid cell growth and function.

Angiogenesis is an important component in the development of thyroid goitre. Vascular endothelial growth factor (VEGF) represents a family of specific endothelial cell mitogens involved in normal angiogenesis and in tumour development. The purpose of this study was to determine the distribution of VEGF in thyroid tissues during goitre formation, and to study the actions of VEGF on the regulation of thymidine incorporation and iodine uptake by thyroid follicular cells. Goitre was induced in adult rats by administration of methimazole together with a low iodine diet. Thyroid from normal or goitrous rats was removed, fixed and sectioned. Immunocytochemistry performed for VEGF using the avidin-biotin system showed that VEGF is present in normal thyroid and is located mainly in the vascular endothelium and interfollicular stromal tissue. After administration of goitrogen for 2 weeks, which caused a two- to threefold increase in thyroid weight, staining of VEGF was less apparent within the interfollicular stroma, but strongly increased throughout the thyroid follicular and endothelial cells. Uptake of [125I] and incorporation of [3H]thymidine by Fisher rat thyroid cells (FRTL-5) were measured after 72 h culture with or without TSH or VEGF, or both. In the absence of TSH, incubation with VEGF caused a significant reduction in [3H]thymidine incorporation, but did not significantly alter [125I] uptake. Incubation with TSH (1 mU/ml) caused a fourfold increase in [3H]thymidine incorporation that was diminished by co-incubation with 10 ng/ml or greater VEGF. Similarly, 10 ng/ml or greater VEGF significantly reduced the ability of TSH to increase [125I] uptake. The antagonistic effects of VEGF on TSH-stimulated [3H]thymidine incorporation or [125I] uptake were significantly reduced in the presence of an anti-VEGF antiserum. A DNA fragment representing mRNA encoding the VEGF receptor, flt-1, was identified in FRTL-5 cells by reverse transcription PCR analysis, and the abundance of this fragment was increased in FRTL-5 cells cultured in the medium containing TSH (1 mU/ml) or fibroblast growth factor (FGF)-2 (25 ng/ml). These results indicated that VEGF and one of its receptors, Flt-1, are present in epithelial cells of the thyroid, and that VEGF could contribute to the regulation of development and function of thyroid epithelial cells.

[Analytical comparison of "paraffin" labeled highly toxic lamp oils with paraffinum perliquidum (DAB 10)]. / Analytischer Vergleich "Paraffin"-deklarierter hochtoxischer Lampenöle mit dem Paraffinum perliquidum (DAB 10).

Coloured and odoriferous lamp oils available as household articles repeatedly lead to ingestion intoxications in children in some cases resulting in a bad course. Labels on containers of lamp oils are misleading, e.g. "pure liquid paraffin" or "without noxious substances", causing considerable misconceptions during therapeutic use by treating doctors. Analyses of four arbitrary chosen lamp oils by GC and GC/MS show that the main components (approx. 98%) are the unbranched saturated hydrocarbons n-decane, n-undecane, n-dodecane, n-tridecane, n-tetradecane. Tests according to the German Pharmacopoeia 10th ed. prove also that "paraffin"-labelled products are not at all identical with medical/pharmaceutical paraffinum perliquidum or liquidum. Lamp oils are low viscous and high toxic petroleum distillates and should be labelled "toxic to humans".

[Development of sex characteristics in relation to nutritional status]. / Entwicklung der sexuellen Reifezeichen in Abhängigkeit vom Ernährungszustand.

Results of anthropometric measurements and determination of the state of sexual maturation in 8703 girls and 8685 boys aged between 8-16 years show that, if these results are related to the nutritional state, the development of the signs of sexual maturity is dependently on the nutritional state.