Proteomics as a Tool to Study Molecular Changes During Plant Morphogenesis In Vitro.

Proteome analysis represents a promising approach for plant tissue culture since it is now possible to identify and quantify proteins on a large scale. Biomarker discovery and the study of the molecular events associated with in vitro plant morphogenesis are considered potential targets for application of proteomics technologies. This chapter describes a protocol for application in in vitro plant material using two proteomics approaches: 2-DE coupled to mass spectrometry and liquid chromatography-linked tandem mass spectrometry.