Dehumanization Through Degendering the Death Row Inmate: A Systematic Review of the Research.

Purpose: To provide an overview of how gender identity is treated in death row research. Methods: By use of a systematic review of 56 peer-reviewed journal articles that were identified as empirical, employing either qualitative or quantitative data, concepts measuring the use of gender and race identity were developed. Results: Findings were presented by the methodology employed, area of research, sample composition, and key concepts which included the use of gendered terminology in titles, abstracts, and manuscripts, as well as by the author(s)'s gender identities. Conclusion: In general, regardless of methodology or area of research, death row research does not account for the effects of gender or intersectional identities unless the sample participants or subject matter pertains to women.

Institutional commitment to combating sexual violence: The practices and policies of U.S. universities.

By presenting institution-level variables of 118 universities across 50 states and the District of Columbia, we provide a descriptive overview of the types of programs and sexual assault-related data. Specifically, we examine correlations between policies and practices related to sexual assault prevention and reports of rape. As expected, we found that universities with policies pertaining to affirmative consent, alcohol, and inclusive definitions of assault, combined with practices like mandatory training and transparency with campus climate survey findings, also have higher reports of sexual assault.

Providing and Receiving Sexual Assault Disclosures: Findings From a Sexually Diverse Sample of Young Adults.

This study utilized a sample of primarily lesbian, gay, bisexual, and queer (LGBQ)-identified young adults from across the United States to pilot test a new instrument, the Reactions Provided to Disclosures Questionnaire (RPDQ), and assess the disclosure experience for both those who provide and those who receive disclosures of sexual assault. Results indicate that the experience of sexual assault disclosure in the LGBQ community is similar to the heterosexual community in that most victims disclose their assaults, most often to a friend, and were most likely to receive the reaction Emotional Support. Victims were also likely to receive the reaction Victim Blaming, especially if they disclosed to formal sources, such as law enforcement, medical, or religious personnel. This study also examined the relationship between the types of assault experienced and disclosure reactions received. Experiencing an anal assault was significantly associated with Victim Blaming reactions. A central aim of this study was to examine how respondents who received (rather than provided) a disclosure reacted, a question not been adequately addressed in prior literature. The RPDQ (a modification of Ullman's Social Reactions Questionnaire), which was piloted here, factored in to five types of reactions: Emotional Support, Affectionate Support, Empathetic Support, Tangible Aid and Information Support, and Egocentric Reactions. Sexual assault survivors were more likely to report that they provided Emotional Support and Affectionate Support after receiving a disclosure than were nonsurvivors.

Gender, Sexual Orientation, and Rape Myth Acceptance: Preliminary Findings From a Sample of Primarily LGBQ-Identified Survey Respondents.

This study is among the first to examine the relationship between sexual orientation and rape myth adherence using a nationwide survey of primarily lesbian, gay, bisexual, and queer (LGBQ) respondents (n = 184). The more established Illinois Rape Myth Acceptance Scale and a modified Male Rape Survey serve as the primary instruments to test both rape myth adherence and instrument-appropriateness. Results suggest that respondents were most likely to support myths that discredit sexual assault allegations or excuse rape as a biological imperative and least likely to support myths related to physical resistance. Consistent with previous work, men exhibited higher levels of rape myth adherence than women. Regarding sexual orientation, respondents who identified as queer consistently exhibited lower levels of rape myth adherence than respondents who identified as gay.

A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells.

In this study, we present a novel technique for the synthesis of complex prokaryotic and eukaryotic proteins by using a continuous-exchange cell-free (CECF) protein synthesis system based on extracts from cultured insect cells. Our approach consists of two basic elements: First, protein synthesis is performed in insect cell lysates which harbor endogenous microsomal vesicles, enabling a translocation of de novo synthesized target proteins into the lumen of the insect vesicles or, in the case of membrane proteins, their embedding into a natural membrane scaffold. Second, cell-free reactions are performed in a two chamber dialysis device for 48 h. The combination of the eukaryotic cell-free translation system based on insect cell extracts and the CECF translation system results in significantly prolonged reaction life times and increased protein yields compared to conventional batch reactions. In this context, we demonstrate the synthesis of various representative model proteins, among them cytosolic proteins, pharmacological relevant membrane proteins and glycosylated proteins in an endotoxin-free environment. Furthermore, the cell-free system used in this study is well-suited for the synthesis of biologically active tissue-type-plasminogen activator, a complex eukaryotic protein harboring multiple disulfide bonds.

Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments.

Open cell-free translation systems based on Escherichia coli cell lysates have successfully been used to produce antibodies and antibody fragments. In this study, we demonstrate the cell-free expression of functional single-chain antibody variable fragments (scFvs) in a eukaryotic and endotoxin-free in vitro translation system based on Spodoptera frugiperda (Sf21) insect cell extracts. Three scFv candidates with different specificities were chosen as models. The first scFv candidate SH527-IIA4 specifically discriminates between its phosphorylated (SMAD2-P) and nonphosphorylated antigens (SMAD2) (where SMAD is mothers against decapentaplegic homolog 2), whereas the second scFv candidate SH527-IIC10 recognizes both, SMAD2-P and SMAD2. The third scFv candidate SH855-C11 binds specifically to a linear epitope of the CXC chemokine receptor type 5. The translocation of antibody fragments into the lumen of endogenous microsomal vesicles, which are contained in the lysate, was facilitated by fusion of scFv genes to the insect cell specific signal sequence of honeybee melittin. We compared the binding capabilities of scFv fragments with and without melittin signal peptide and detected that translocated scFv fragments were highly functional, whereas scFvs synthesized in the cytosol of the cell extract showed strongly decreased binding capabilities. Additionally, we describe a cell-free protein synthesis method for the incorporation of noncanonical amino acids into scFv molecules in eukaryotic cell lysates. We demonstrate the successful cotranslational labeling of de novo synthesized scFv molecules with fluorescent amino acids, using residue-specific as well as site-specific labeling.

Erythropoietin modulates autophagy signaling in the developing rat brain in an in vivo model of oxygen-toxicity.

Autophagy is a self-degradative process that involves turnover and recycling of cytoplasmic components in healthy and diseased tissue. Autophagy has been shown to be protective at the early stages of programmed cell death but it can also promote apoptosis under certain conditions. Earlier we demonstrated that oxygen contributes to the pathogenesis of neonatal brain damage, which can be ameliorated by intervention with recombinant human erythropoietin (rhEpo). Extrinsic- and intrinsic apoptotic pathways are involved in oxygen induced neurotoxicity but the role of autophagy in this model is unclear. We analyzed the expression of autophagy activity markers in the immature rodent brain after exposure to elevated oxygen concentrations. We observed a hyperoxia-exposure dependent regulation of autophagy-related gene (Atg) proteins Atg3, 5, 12, Beclin-1, microtubule-associated protein 1 light chain 3 (LC3), LC3A-II, and LC3B-II which are all key autophagy activity proteins. Interestingly, a single injection with rhEpo at the onset of hyperoxia counteracted these oxygen-mediated effects. Our results indicate that rhEpo generates its protective effect by modifying the key autophagy activity proteins.