ABSTRACT
Members of the genus Treponema have been implicated as possible aetiologic agents of periodontal disease. Previously developed murine monoclonal antibodies (MAbs) to two Treponema denticola strains were characterized by an indirect fluorescent-antibody (IFA) technique. Fifteen T. denticola strains and 32 other bacteria commonly isolated from periodontal pockets were used in the screening process. Two monoclonal antibodies to T. denticola ATCC 33521, serotype B, reacted with five T. denticola isolates, and one monoclonal antibody to T. denticola ATCC 33520, serotype c, reacted with two T. denticola isolates. There was no cross-reactivity between MAbs to the two serotypes. Preliminary tests of the MAbs on human periodontal samples show that they are useful in detecting these bacteria in clinical samples.
Subject(s)
Antibodies, Bacterial/immunology , Antibodies, Monoclonal/immunology , Periodontal Diseases/microbiology , Treponema/immunology , Antibody Specificity , Cross Reactions , Enzyme-Linked Immunosorbent Assay , Fluorescent Antibody Technique , Humans , Serotyping , Species Specificity , Treponema/isolation & purificationABSTRACT
Since burs used in OMF surgery are a source of microbial contamination, sterilisation of the burs is an important aspect of infection control. A two-part study was performed to evaluate the efficacy of using the glass bead steriliser to sterilise long-shank burs. The laboratory and clinical studies indicated that the glass bead steriliser was effective in sterilising long-shank burs in 60 s.
Subject(s)
Glass , Sterilization/methods , Surgery, Oral/instrumentation , Alloys , Bacillus subtilis , Chlorhexidine/analogs & derivatives , Chlorhexidine/therapeutic use , Equipment Contamination/prevention & control , Humans , In Vitro Techniques , Time Factors , Tooth Extraction/instrumentationABSTRACT
Infection control in dentistry focuses on the prevention of cross-contamination among patients, dentists, and dental auxilary personnel. This study evaluated the bactericidal effect of a dental gypsum material containing 0.25% chloramine-T on oral irreversible hydrocolloid impressions and dental casts. Eighty irreversible hydrocolloid impressions from 40 human volunteers were prepared. Forty impressions were poured with the disinfectant dental stone and a similar number were poured with a comparable, nondisinfectant stone. After setting for 60 minutes, the impressions and separated casts were sampled for bacterial contamination. The disinfectant stone inhibited bacterial growth in 39 of 40 impressions and casts, whereas all casts and impressions poured with the nondisinfectant stone were contaminated. The disinfectant dental stone containing chloramine-T was effective in eliminating bacterial contamination of both the irreversible hydrocolloid impression and stone cast.
Subject(s)
Alginates , Anti-Infective Agents, Local/pharmacology , Bacteria/drug effects , Calcium Sulfate/pharmacology , Chloramines/pharmacology , Disinfectants/pharmacology , Models, Dental , Tosyl Compounds , Chloramines/administration & dosage , Humans , Mouth/microbiology , Therapeutic IrrigationABSTRACT
The intravenous inoculation of adult mice with virulent group B streptococci serotype Ia resulted in fulminating sepsis with extensive colonization of the lungs and kidneys. The time course of the infection lasting 24 to 40 h, extensive pulmonary colonization, and resistance of the type Ia organism to phagocytosis in the absence of specific antibody suggest that mice are an appropriate model for the study of early onset streptococcal infection of human neonates.
