ABSTRACT
High-frequency scanning acoustic microscopy (SAM) was used for investigation of acoustic impedance and 3D-surface topography of full alginate microspheres that act as model of artificial biological cells. Elasto-mechanical properties of the investigated specimens have been characterized by acoustic impedance. Mean surface impedance of microspheres (diameter: 300 microm) was measured with SAM at 900 MHz with a spatial resolution of 1.5 microm. The sensitivity and reproducibility of SAM had to be increased considerable to receive and quantify signals in the very low impedance region. The multilayer analysis method was used to get quantitative data of acoustic impedance with SAM at a microscopic level. 3D images show details of structure and surface topography. As a reference, bulk measurements were performed on full alginate cylinders. The acoustical impedance and the mechanical stiffness c(11) were obtained from mass density and longitudinal ultrasound velocity at 6 MHz. The impedances received with both methods are in close agreement. The results demonstrate the SAM as a powerful tool for characterizing mechano-elastical parameters as well as surface structure and topography of microspheres with high spatial resolution.
