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Baggett, J J; Shaw, J D; Sciambi, C J; Watson, H A; Wendland, B.

Curr Protoc Cell Biol ; Chapter 4: Unit 4.13, 2003 Nov.

Article in English | MEDLINE | ID: mdl-18228435

ABSTRACT

This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.

Subject(s)

Fluorescent Dyes/metabolism , Saccharomyces cerevisiae/cytology , Staining and Labeling/methods , Actins/metabolism , Benzenesulfonates/metabolism , Chitin/metabolism , Cytoskeleton/metabolism , Endocytosis , Fluoresceins/metabolism , Fluorescent Antibody Technique , Green Fluorescent Proteins/metabolism , Isoquinolines/metabolism , Microbial Viability , Phalloidine/metabolism , Protein Transport , Pyridinium Compounds/metabolism , Quaternary Ammonium Compounds/metabolism , Quinacrine/metabolism , Recombinant Fusion Proteins/metabolism , Rhodamines/metabolism , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Spheroplasts/metabolism , Time Factors , Vacuoles/metabolism

ABSTRACT

Subject(s)

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