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1.
Acta Crystallogr F Struct Biol Commun ; 77(Pt 5): 140-147, 2021 May 01.
Article in English | MEDLINE | ID: mdl-33949974

ABSTRACT

The Atg8 protein family comprises the GABA type A receptor-associated proteins (GABARAPs) and microtubule-associated protein 1 light chains 3 (MAP1LC3s) that are essential mediators of autophagy. The LC3-interacting region (LIR) motifs of autophagy receptors and adaptors bind Atg8 proteins to promote autophagosome formation, cargo recruitment, and autophagosome closure and fusion to lysosomes. A crystal structure of human GABARAPL2 has been published [PDB entry 4co7; Ma et al. (2015), Biochemistry, 54, 5469-5479]. This was crystallized in space group P21 with a monoclinic angle of 90° and shows a pseudomerohedral twinning pathology. This article reports a new, untwinned GABARAPL2 crystal form, also in space group P21, but with a 98° monoclinic angle. No major conformational differences were observed between the structures. In the structure described here, the C-terminal Phe117 binds into the LIR docking site (LDS) of a neighbouring molecule within the asymmetric unit, as observed in the previously reported structure. This crystal contact blocks the LDS for co-crystallization with ligands. Phe117 of GABARAPL2 is normally removed during biological processing by Atg4 family proteases. These data indicate that to establish interactions with the LIR, Phe117 should be removed to eliminate the crystal contact and liberate the LDS for co-crystallization with LIR peptides.


Subject(s)
Autophagy-Related Protein 8 Family/metabolism , Microtubule-Associated Proteins/metabolism , Peptide Fragments/metabolism , Phenylalanine/metabolism , Autophagy-Related Protein 8 Family/chemistry , Crystallography, X-Ray , Humans , Microtubule-Associated Proteins/chemistry , Models, Molecular , Peptide Fragments/chemistry , Phenylalanine/chemistry , Protein Binding , Protein Conformation
2.
Nat Commun ; 9(1): 4976, 2018 11 26.
Article in English | MEDLINE | ID: mdl-30478310

ABSTRACT

Intrinsic apoptosis is critical to prevent tumor formation and is engaged by many anti-cancer agents to eliminate tumor cells. BAX and BAK, the two essential mediators of apoptosis, are thought to be regulated through similar mechanisms and act redundantly to drive apoptotic cell death. From an unbiased genome-wide CRISPR/Cas9 screen, we identified VDAC2 (voltage-dependent anion channel 2) as important for BAX, but not BAK, to function. Genetic deletion of VDAC2 abrogated the association of BAX and BAK with mitochondrial complexes containing VDAC1, VDAC2, and VDAC3, but only inhibited BAX apoptotic function. Deleting VDAC2 phenocopied the loss of BAX in impairing both the killing of tumor cells by anti-cancer agents and the ability to suppress tumor formation. Together, our studies show that efficient BAX-mediated apoptosis depends on VDAC2, and reveal a striking difference in how BAX and BAK are functionally impacted by their interactions with VDAC2.


Subject(s)
Apoptosis , Carcinogenesis/metabolism , Carcinogenesis/pathology , Voltage-Dependent Anion Channel 2/metabolism , bcl-2-Associated X Protein/metabolism , Animals , CRISPR-Cas Systems/genetics , Embryonic Development , HCT116 Cells , HeLa Cells , Humans , Mice, Inbred C57BL , Mitochondria/metabolism , Promoter Regions, Genetic/genetics , bcl-2 Homologous Antagonist-Killer Protein/metabolism
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