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1.
Physiol Rep ; 6(22): e13913, 2018 Nov.
Article in English | MEDLINE | ID: mdl-30467998

ABSTRACT

Adenosine operating in the nucleus of the solitary tract (NTS) may inhibit or facilitate neurotransmitter release from nerve terminals and directly inhibit or facilitate central neurons via A1 and A2a pre- and postsynaptic receptors, respectively. However, adenosine A2a receptors, may also activate GABA-ergic neurons/terminals which in turn inhibit glutamatergic transmission in the NTS network. Our previous studies showed that adenosine operating via both A1 (inhibitor) and A2a (activator) receptors powerfully inhibits the cardiopulmonary chemoreflex (CCR) at the level of the caudal NTS. A1 receptors most likely inhibit glutamate release in the CCR network, whereas A2a receptors facilitate NTS GABA-ergic mechanisms which in turn inhibit CCR glutamatergic transmission. Therefore, we hypothesized that A2a receptors are located on NTS GABA-ergic neurons/terminals whereas A1 receptors may be located on NTS glutamatergic neurons/terminals. We investigated this hypothesis using double immunofluorescent staining for A2a or A1 adenosine receptors and GABA synthesizing enzyme, GAD67, in 30 µm thick, floating, medullary rat sections. We found that A2a adenosine receptors are localized within the GABA-ergic cells in the caudal NTS, whereas A1 adenosine receptors are absent from these neurons. Instead, A1 receptors were located on non-GABA-ergic (likely glutamatergic) neurons/terminals in the caudal NTS. These data support our functional findings and the hypothesis that adenosine A2a, but not A1 receptors are located on GABA-ergic neurons.


Subject(s)
GABAergic Neurons/metabolism , Receptor, Adenosine A1/metabolism , Receptor, Adenosine A2A/metabolism , Reflex , Solitary Nucleus/physiology , Animals , GABAergic Neurons/physiology , Rats , Rats, Sprague-Dawley , Receptor, Adenosine A1/genetics , Receptor, Adenosine A2A/genetics , Solitary Nucleus/cytology , Solitary Nucleus/metabolism
2.
Am J Physiol Heart Circ Physiol ; 309(1): H185-97, 2015 Jul 01.
Article in English | MEDLINE | ID: mdl-25910812

ABSTRACT

Adenosine is a powerful central neuromodulator acting via opposing A1 (inhibitor) and A2a (activator) receptors. However, in the nucleus of the solitary tract (NTS), both adenosine receptor subtypes attenuate cardiopulmonary chemoreflex (CCR) sympathoinhibition of renal, adrenal, and lumbar sympathetic nerve activity and attenuate reflex decreases in arterial pressure and heart rate. Adenosine A1 receptors inhibit glutamatergic transmission in the CCR pathway, whereas adenosine A2a receptors most likely facilitate release of an unknown inhibitory neurotransmitter, which, in turn, inhibits the CCR. We hypothesized that adenosine A2a receptors inhibit the CCR via facilitation of GABA release in the NTS. In urethane-chloralose-anesthetized rats (n = 51), we compared regional sympathetic responses evoked by stimulation of the CCR with right atrial injections of the 5-HT3 receptor agonist phenylbiguanide (1-8 µg/kg) before and after selective stimulation of NTS adenosine A2a receptors [microinjections into the NTS of CGS-21680 (20 pmol/50 nl)] preceded by blockade of GABAA or GABAB receptors in the NTS [bicuculline (10 pmol/100 nl) or SCH-50911 (1 nmol/100 nl)]. Blockade of GABAA receptors virtually abolished adenosine A2a receptor-mediated inhibition of the CCR. GABAB receptors had much weaker but significant effects. These effects were similar for the different sympathetic outputs. We conclude that stimulation of NTS adenosine A2a receptors inhibits CCR-evoked hemodynamic and regional sympathetic reflex responses via a GABA-ergic mechanism.


Subject(s)
Biguanides/pharmacology , Receptor, Adenosine A2A/metabolism , Receptors, GABA-A/metabolism , Receptors, GABA-B/metabolism , Reflex/physiology , Serotonin Receptor Agonists/pharmacology , Solitary Nucleus/metabolism , Sympathetic Nervous System/metabolism , Adenosine/analogs & derivatives , Adenosine/pharmacology , Adenosine A2 Receptor Agonists/pharmacology , Animals , Bicuculline/pharmacology , GABA Antagonists/pharmacology , Heart/drug effects , Microinjections , Morpholines/pharmacology , Phenethylamines/pharmacology , Rats , Reflex/drug effects , Sympathetic Nervous System/drug effects
3.
Am J Physiol Heart Circ Physiol ; 307(6): H904-9, 2014 Sep 15.
Article in English | MEDLINE | ID: mdl-25063794

ABSTRACT

Selective stimulation of inhibitory A1 and facilitatory A2a adenosine receptor subtypes located in the nucleus of the solitary tract (NTS) powerfully inhibits cardiopulmonary chemoreflex (CCR) control of regional sympathetic outputs via different mechanisms: direct inhibition of glutamate release and facilitation of an inhibitory neurotransmitter release, respectively. However, it remains unknown whether adenosine naturally released into the NTS has similar inhibitory effects on the CCR as the exogenous agonists do. Our previous study showed that adenosine is released into the NTS during severe hemorrhage and contributes to reciprocal changes of renal (decreases) and adrenal (increases) sympathetic nerve activity observed in this setting. Both A1 and A2a adenosine receptors are involved. Therefore, we tested the hypothesis that, during severe hemorrhage, CCR control of the two sympathetic outputs is attenuated by adenosine naturally released into the NTS. We compared renal and adrenal sympathoinhibitory responses evoked by right atrial injections of 5HT3 receptor agonist phenylbiguanide (2-8 µg/kg) under control conditions, during hemorrhage, and during hemorrhage preceded by blockade of NTS adenosine receptors with bilateral microinjections of 8-(p-sulfophenyl) theophylline (1 nmol/100 nl) in urethane/chloralose anesthetized rats. CCR-mediated inhibition of renal and adrenal sympathetic activity was significantly attenuated during severe hemorrhage despite reciprocal changes in the baseline activity levels, and this attenuation was removed by bilateral blockade of adenosine receptors in the caudal NTS. This confirmed that adenosine endogenously released into the NTS has a similar modulatory effect on integration of cardiovascular reflexes as stimulation of NTS adenosine receptors with exogenous agonists.


Subject(s)
Cardiovascular System/innervation , Chemoreceptor Cells/metabolism , Hemorrhage/metabolism , Receptor, Adenosine A1/metabolism , Receptor, Adenosine A2A/metabolism , Reflex , Solitary Nucleus/metabolism , Sympathetic Nervous System/metabolism , Adenosine/metabolism , Adrenal Glands/innervation , Animals , Blood Pressure , Disease Models, Animal , Heart Rate , Hemorrhage/physiopathology , Hypotension/metabolism , Hypotension/physiopathology , Kidney/innervation , Male , Neural Inhibition , Purinergic P1 Receptor Antagonists/pharmacology , Rats , Rats, Sprague-Dawley , Receptor, Adenosine A1/drug effects , Receptor, Adenosine A2A/drug effects , Receptors, Serotonin, 5-HT3/drug effects , Receptors, Serotonin, 5-HT3/metabolism , Reflex/drug effects , Serotonin 5-HT3 Receptor Agonists/pharmacology , Severity of Illness Index , Solitary Nucleus/drug effects , Solitary Nucleus/physiopathology , Sympathetic Nervous System/drug effects , Sympathetic Nervous System/physiopathology , Time Factors
4.
Auton Neurosci ; 180: 32-42, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24216055

ABSTRACT

Previously we have shown that stimulation of inhibitory A1 adenosine receptors located in the nucleus tractus solitarii (NTS) attenuates cardiopulmonary chemoreflex (CCR) evoked inhibition of renal, adrenal and lumbar sympathetic nerve activity and reflex decreases in arterial pressure and heart rate. Activation of facilitatory A2a adenosine receptors, which dominate over A1 receptors in the NTS, contrastingly alters baseline activity of regional sympathetic outputs: it decreases renal, increases adrenal and does not change lumbar nerve activity. Considering that NTS A2a receptors may facilitate release of inhibitory transmitters we hypothesized that A2a receptors will act in concert with A1 receptors differentially inhibiting regional sympathetic CCR responses (adrenal>lumbar>renal). In urethane/chloralose anesthetized rats (n=38) we compared regional sympathetic responses evoked by stimulation of the CCR with right atrial injections of serotonin 5HT3 receptor agonist, phenylbiguanide, (1-8µg/kg) before and after selective stimulation, blockade or combined blockade and stimulation of NTS A2a adenosine receptors (microinjections into the NTS of CGS-21680 0.2-20pmol/50nl, ZM-241385 40pmol/100nl or ZM-241385+CGS-21680, respectively). We found that stimulation of A2a adenosine receptors uniformly inhibited the regional sympathetic and hemodynamic reflex responses and this effect was abolished by the selective blockade of NTS A2a receptors. This indicates that A2a receptor triggered inhibition of CCR responses and the contrasting shifts in baseline sympathetic activity are mediated via different mechanisms. These data implicate that stimulation of NTS A2a receptors triggers unknown inhibitory mechanism(s) which in turn inhibit transmission in the CCR pathway when adenosine is released into the NTS during severe hypotension.


Subject(s)
Adenosine/physiology , Blood Pressure/physiology , Heart Conduction System/physiology , Heart Rate/physiology , Receptors, Adrenergic, alpha-2/physiology , Solitary Nucleus/physiology , Sympathetic Nervous System/physiology , Adenosine/administration & dosage , Adenosine/analogs & derivatives , Adenosine/pharmacology , Adrenal Glands/innervation , Animals , Biguanides/pharmacology , Chemoreceptor Cells/drug effects , Chemoreceptor Cells/physiology , Heart Atria/drug effects , Heart Conduction System/drug effects , Heart Rate/drug effects , Hypotension/physiopathology , Kidney/innervation , Lumbosacral Region/innervation , Male , Microinjections , Models, Neurological , Phenethylamines/administration & dosage , Phenethylamines/pharmacology , Rats , Rats, Sprague-Dawley , Receptors, Adrenergic, alpha-2/drug effects , Reflex/drug effects , Reflex/physiology , Serotonin 5-HT3 Receptor Agonists/pharmacology , Sympathetic Nervous System/drug effects , Sympathetic Nervous System/physiopathology , Triazines/pharmacology , Triazoles/pharmacology
5.
Am J Physiol Regul Integr Comp Physiol ; 303(5): R539-50, 2012 Sep 01.
Article in English | MEDLINE | ID: mdl-22814665

ABSTRACT

Previously we have shown that adenosine operating via the A(1) receptor subtype may inhibit glutamatergic transmission in the baroreflex arc within the nucleus of the solitary tract (NTS) and differentially increase renal (RSNA), preganglionic adrenal (pre-ASNA), and lumbar (LSNA) sympathetic nerve activity (ASNA>RSNA≥LSNA). Since the cardiopulmonary chemoreflex and the arterial baroreflex are mediated via similar medullary pathways, and glutamate is a primary transmitter in both pathways, it is likely that adenosine operating via A(1) receptors in the NTS may differentially inhibit regional sympathetic responses evoked by activation of cardiopulmonary chemoreceptors. Therefore, in urethane-chloralose-anesthetized rats (n = 37) we compared regional sympathoinhibition evoked by the cardiopulmonary chemoreflex (activated with right atrial injections of serotonin 5HT(3) receptor agonist phenylbiguanide, PBG, 1-8 µg/kg) before and after selective stimulation of NTS A(1) adenosine receptors [microinjections of N(6)-cyclopentyl adenosine (CPA), 0.033-330 pmol/50 nl]. Activation of cardiopulmonary chemoreceptors evoked differential, dose-dependent sympathoinhibition (RSNA>ASNA>LSNA), and decreases in arterial pressure and heart rate. These differential sympathetic responses were uniformly attenuated in dose-dependent manner by microinjections of CPA into the NTS. Volume control (n = 11) and blockade of adenosine receptor subtypes in the NTS via 8-(p-sulfophenyl)theophylline (8-SPT, 1 nmol in 100 nl) (n = 9) did not affect the reflex responses. We conclude that activation of NTS A(1) adenosine receptors uniformly inhibits neural and cardiovascular cardiopulmonary chemoreflex responses. A(1) adenosine receptors have no tonic modulatory effect on this reflex under normal conditions. However, when adenosine is released into the NTS (i.e., during stress or severe hypotension/ischemia), it may serve as negative feedback regulator for depressor and sympathoinhibitory reflexes integrated in the NTS.


Subject(s)
Cardiovascular Physiological Phenomena , Chemoreceptor Cells/physiology , Lung/physiology , Receptor, Adenosine A1/physiology , Solitary Nucleus/physiology , Sympathetic Nervous System/physiology , Adenosine/analogs & derivatives , Adenosine/pharmacology , Animals , Biguanides/pharmacology , Blood Pressure/physiology , Cardiovascular Physiological Phenomena/drug effects , Chemoreceptor Cells/drug effects , Dose-Response Relationship, Drug , Feedback, Physiological/physiology , Heart Rate/physiology , Lung/drug effects , Male , Models, Animal , Rats , Rats, Sprague-Dawley , Receptor, Adenosine A1/drug effects , Serotonin Receptor Agonists/pharmacology , Solitary Nucleus/drug effects , Theophylline/analogs & derivatives , Theophylline/pharmacology
6.
Am J Physiol Regul Integr Comp Physiol ; 300(5): R1230-40, 2011 May.
Article in English | MEDLINE | ID: mdl-21346240

ABSTRACT

Under acute and chronic conditions, the sympathetic nervous system can be activated in a differential and even selective manner. Activation of the rostral ventrolateral medulla (RVLM) has been implicated in differential control of sympathetic outputs based on evidence primarily in the cat. Although several studies indicate that differential control of sympathetic outflow occurs in other species, only a few studies have addressed whether the RVLM is capable of producing varying patterns of sympathetic activation in the rat. Therefore, the purpose of the present study was to determine whether activation of the RVLM results in simultaneous and differential increases in preganglionic adrenal (pre-ASNA), renal (RSNA), and lumbar (LSNA) sympathetic nerve activities. In urethane-chloralose anesthetized rats, pre-ASNA, RSNA, and LSNA were recorded simultaneously in all animals. Microinjections of selected concentrations and volumes of glutamate increased pre-ASNA, RSNA, and LSNA concurrently and differentially. Pre-ASNA and RSNA (in most cases) exhibited greater increases compared with LSNA on a percentage basis. By varying the volume or location of the glutamate microinjections, we also identified individual examples of differential and selective activation of these nerves. Decreases in arterial pressure or bilateral blockade of RVLM GABA(A) receptors also revealed differential activation, with the latter having a 3- to 4-fold greater effect on sympathetic activity. Our data provide evidence that activation of the rat RVLM increases renal, lumbar, and preganglionic adrenal sympathetic nerve activities concurrently, differentially, and, in some cases, selectively.


Subject(s)
Adrenal Glands/innervation , Autonomic Fibers, Preganglionic/physiology , Kidney/innervation , Lumbosacral Plexus/physiology , Medulla Oblongata/physiology , Muscle, Skeletal/blood supply , Sympathetic Nervous System/physiology , Action Potentials , Analysis of Variance , Anesthesia, General , Animals , Autonomic Fibers, Preganglionic/drug effects , Bicuculline/administration & dosage , Blood Pressure , Blood Vessels/innervation , Dose-Response Relationship, Drug , Excitatory Amino Acid Agonists/administration & dosage , GABA-A Receptor Antagonists/administration & dosage , Glutamic Acid/administration & dosage , Hindlimb , Lumbosacral Plexus/drug effects , Male , Medulla Oblongata/drug effects , Microinjections , Nitroprusside/administration & dosage , Rats , Rats, Sprague-Dawley , Sympathetic Nervous System/drug effects , Time Factors , Vasodilator Agents/administration & dosage
7.
Am J Physiol Regul Integr Comp Physiol ; 300(3): R744-55, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21148476

ABSTRACT

Our previous studies showed that stimulation of adenosine A(1) receptors located in the nucleus of the solitary tract (NTS) exerts counteracting effects on the iliac vascular bed: activation of the adrenal medulla and ß-adrenergic vasodilation vs. sympathetic and vasopressinergic vasoconstriction. Because NTS A(1) adenosine receptors inhibit baroreflex transmission in the NTS and contribute to the pressor component of the HDR, we hypothesized that these receptors also contribute to the redistribution of blood from the visceral to the muscle vasculature via prevailing sympathetic and vasopressinergic vasoconstriction in the visceral (renal and mesenteric) vascular beds and prevailing ß-adrenergic vasodilation in the somatic (iliac) vasculature. To test this hypothesis, we compared the A(1) adenosine-receptor-mediated effects of each vasoactive factor triggered by NTS A(1) adenosine receptor stimulation [N(6)-cyclopentyladenosine (CPA), 330 pmol in 50 nl] on the regional vascular responses in urethane/chloralose-anesthetized rats. The single-factor effects were separated using adrenalectomy, ß-adrenergic blockade, V(1) vasopressin receptor blockade, and sinoaortic denervation. In intact animals, initial vasodilation was followed by large, sustained vasoconstriction with smaller responses observed in renal vs. mesenteric and iliac vascular beds. The initial ß-adrenergic vasodilation prevailed in the iliac vs. mesenteric and renal vasculature. The large and sustained vasopressinergic vasoconstriction was similar in all vascular beds. Small sympathetic vasoconstriction was observed only in the iliac vasculature in this setting. We conclude that, although A(1) adenosine-receptor-mediated ß-adrenergic vasodilation may contribute to the redistribution of blood from the visceral to the muscle vasculature, this effect is overridden by sympathetic and vasopressinergic vasoconstriction.


Subject(s)
Adrenergic Fibers/metabolism , Hemodynamics , Iliac Artery/innervation , Mesenteric Arteries/innervation , Muscle, Skeletal/blood supply , Receptor, Adenosine A1/metabolism , Receptors, Adrenergic, beta/metabolism , Renal Artery/innervation , Solitary Nucleus/metabolism , Adenosine A1 Receptor Agonists/pharmacology , Adrenal Medulla/innervation , Adrenal Medulla/metabolism , Adrenal Medulla/surgery , Adrenalectomy , Adrenergic Fibers/drug effects , Adrenergic beta-Antagonists/pharmacology , Animals , Antidiuretic Hormone Receptor Antagonists , Autonomic Denervation , Hemodynamics/drug effects , Hormone Antagonists/pharmacology , Iliac Artery/drug effects , Iliac Artery/metabolism , Male , Mesenteric Arteries/drug effects , Mesenteric Arteries/metabolism , Rats , Rats, Sprague-Dawley , Receptor, Adenosine A1/drug effects , Receptors, Adrenergic, beta/drug effects , Receptors, Vasopressin/metabolism , Regional Blood Flow , Renal Artery/drug effects , Renal Artery/metabolism , Renal Circulation , Solitary Nucleus/drug effects , Splanchnic Circulation , Time Factors , Vasoconstriction , Vasodilation , Vasopressins/metabolism
8.
Am J Physiol Heart Circ Physiol ; 297(5): H1661-72, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19749166

ABSTRACT

Our previous study showed that stimulation of adenosine A(1) receptors located in the nucleus of the solitary tract (NTS) exerts counteracting effects on the iliac vascular bed: activation of the adrenal medulla and beta-adrenergic vasodilation versus vasoconstriction mediated by neural and unknown humoral factors. In the present study we investigated the relative contribution of three major potential humoral vasoconstrictors: vasopressin, angiotensin II, and norepinephrine in this response. In urethane-chloralose anesthetized rats we compared the integral changes in iliac vascular conductance evoked by microinjections into the NTS of the selective A(1) receptor agonist N(6)-cyclopentyladenosine (CPA; 330 pmol in 50 nl) in intact (Int) animals and following: V(1) vasopressin receptor blockade (VX), angiotensin II AT(1) receptor blockade (ATX), bilateral adrenalectomy + ganglionic blockade (ADX + GX; which eliminated the potential increases in circulating norepinephrine and epinephrine), ADX + GX + VX and ADX + GX + VX + ATX. In Int animals, stimulation of NTS A(1) adenosine receptors evoked typical variable responses with prevailing pressor and vasoconstrictor effects. VX reversed the responses to depressor ones. ATX did not significantly alter the responses. ADX + GX accentuated pressor and vasoconstrictor responses, whereas ADX + GX + VX and ADX + GX + VX + ATX virtually abolished the responses. Stimulation of NTS A(1) adenosine receptors increased circulating vasopressin over fourfold (26.4 + or - 10.4 vs. 117.0 + or - 19 pg/ml). These data strongly suggest that vasopressin is a major vasoconstrictor factor opposing beta-adrenergic vasodilation in iliac vascular responses triggered by stimulation of NTS A(1) adenosine receptors, whereas angiotensin II and norepinephrine do not contribute significantly to the vasoconstrictor responses.


Subject(s)
Angiotensin II/metabolism , Baroreflex , Iliac Artery/innervation , Muscle, Skeletal/blood supply , Norepinephrine/metabolism , Receptor, Adenosine A1/metabolism , Solitary Nucleus/metabolism , Vasoconstriction , Vasopressins/metabolism , Adenosine/administration & dosage , Adenosine/analogs & derivatives , Adenosine A1 Receptor Agonists , Adrenalectomy , Angiotensin II Type 1 Receptor Blockers/administration & dosage , Animals , Antidiuretic Hormone Receptor Antagonists , Arginine Vasopressin/administration & dosage , Arginine Vasopressin/analogs & derivatives , Baroreflex/drug effects , Blood Pressure , Heart Rate , Hindlimb , Losartan/administration & dosage , Male , Microinjections , Rats , Rats, Sprague-Dawley , Receptor, Angiotensin, Type 1/drug effects , Receptor, Angiotensin, Type 1/metabolism , Receptors, Vasopressin/metabolism , Regional Blood Flow , Solitary Nucleus/drug effects , Sympathectomy , Time Factors , Vasoconstriction/drug effects , Vasopressins/blood
9.
Am J Physiol Heart Circ Physiol ; 296(4): H1058-68, 2009 Apr.
Article in English | MEDLINE | ID: mdl-19202001

ABSTRACT

The role of nucleus of solitary tract (NTS) A(2a) adenosine receptors in baroreflex mechanisms is controversial. Stimulation of these receptors releases glutamate within the NTS and elicits baroreflex-like decreases in mean arterial pressure (MAP), heart rate (HR), and renal sympathetic nerve activity (RSNA), whereas inhibition of these receptors attenuates HR baroreflex responses. In contrast, stimulation of NTS A(2a) adenosine receptors increases preganglionic adrenal sympathetic nerve activity (pre-ASNA), and the depressor and sympathoinhibitory responses are not markedly affected by sinoaortic denervation and blockade of NTS glutamatergic transmission. To elucidate the role of NTS A(2a) adenosine receptors in baroreflex function, we compared full baroreflex stimulus-response curves for HR, RSNA, and pre-ASNA (intravenous nitroprusside/phenylephrine) before and after bilateral NTS microinjections of selective adenosine A(2a) receptor agonist (CGS-21680; 2.0, 20 pmol/50 nl), selective A(2a) receptor antagonist (ZM-241385; 40 pmol/100 nl), and nonselective A(1) + A(2a) receptor antagonist (8-SPT; 1 nmol/100 nl) in urethane/alpha-chloralose anesthetized rats. Activation of A(2a) receptors decreased the range, upper plateau, and gain of baroreflex-response curves for RSNA, whereas these parameters all increased for pre-ASNA, consistent with direct effects of the agonist on regional sympathetic activity. However, no resetting of baroreflex-response curves along the MAP axis occurred despite the marked decreases in baseline MAP. The antagonists had no marked effects on baseline variables or baroreflex-response functions. We conclude that the activation of NTS A(2a) adenosine receptors differentially alters baroreflex control of HR, RSNA, and pre-ASNA mostly via non-baroreflex mechanism(s), and these receptors have virtually no tonic action on baroreflex control of these sympathetic outputs.


Subject(s)
Adrenal Glands/innervation , Baroreflex/physiology , Kidney/innervation , Receptor, Adenosine A2A/metabolism , Solitary Nucleus/metabolism , Sympathetic Nervous System/physiology , Adenosine/analogs & derivatives , Adenosine/pharmacology , Adenosine A2 Receptor Agonists , Adenosine A2 Receptor Antagonists , Animals , Antihypertensive Agents/pharmacology , Blood Pressure/physiology , Heart Rate/physiology , Male , Phenethylamines/pharmacology , Rats , Rats, Sprague-Dawley , Receptor, Adenosine A1/metabolism , Theophylline/analogs & derivatives , Theophylline/pharmacology , Triazines/pharmacology , Triazoles/pharmacology
10.
Am J Physiol Heart Circ Physiol ; 294(1): H172-82, 2008 Jan.
Article in English | MEDLINE | ID: mdl-17982009

ABSTRACT

Previously we showed that pressor and differential regional sympathoexcitatory responses (adrenal > renal >/= lumbar) evoked by stimulation of A(1) adenosine receptors located in the nucleus of the solitary tract (NTS) were attenuated/abolished by baroreceptor denervation or blockade of glutamatergic transmission in the NTS, suggesting A(1) receptor-elicited inhibition of glutamatergic transmission in baroreflex pathways. Therefore we tested the hypothesis that stimulation of NTS A(1) adenosine receptors differentially inhibits/resets baroreflex responses of preganglionic adrenal (pre-ASNA), renal (RSNA), and lumbar (LSNA) sympathetic nerve activity. In urethane-chloralose-anesthetized male Sprague-Dawley rats (n = 65) we compared baroreflex-response curves (iv nitroprusside and phenylephrine) evoked before and after bilateral microinjections into the NTS of A(1) adenosine receptor agonist (N(6)-cyclopentyladenosine, CPA; 0.033-330 pmol/50 nl). CPA evoked typical dose-dependent pressor and differential sympathoexcitatory responses and similarly shifted baroreflex curves for pre-ASNA, RSNA, and LSNA toward higher mean arterial pressure (MAP) in a dose-dependent manner; the maximal shifts were 52.6 +/- 2.8, 48.0 +/- 3.6, and 56.8 +/- 6.7 mmHg for pre-ASNA, RSNA, and LSNA, respectively. These shifts were not a result of simple baroreceptor resetting because they were two to three times greater than respective increases in baseline MAP evoked by CPA. Baroreflex curves for pre-ASNA were additionally shifted upward: the maximal increases of upper and lower plateaus were 41.8 +/- 16.4% and 45.3 +/- 8.7%, respectively. Maximal gain (%/mmHg) measured before vs. after CPA increased for pre-ASNA (3.0 +/- 0.6 vs. 4.9 +/- 1.3), decreased for RSNA (4.1 +/- 0.6 vs. 2.3 +/- 0.3), and remained unaltered for LSNA (2.1 +/- 0.2 vs. 2.0 +/- 0.1). Vehicle control did not alter the baroreflex curves. We conclude that the activation of NTS A(1) adenosine receptors differentially inhibits/resets baroreflex control of regional sympathetic outputs.


Subject(s)
Adenosine A1 Receptor Agonists , Adenosine/analogs & derivatives , Autonomic Fibers, Preganglionic/drug effects , Baroreflex/drug effects , Neural Inhibition/drug effects , Solitary Nucleus/drug effects , Sympathetic Nervous System/drug effects , Adenosine/pharmacology , Adrenal Glands/drug effects , Adrenal Glands/innervation , Animals , Autonomic Fibers, Preganglionic/metabolism , Blood Pressure/drug effects , Dose-Response Relationship, Drug , Heart Rate/drug effects , Kidney/drug effects , Kidney/innervation , Lumbosacral Plexus/drug effects , Male , Nitroprusside/pharmacology , Phenylephrine/pharmacology , Rats , Rats, Sprague-Dawley , Receptor, Adenosine A1/metabolism , Research Design , Solitary Nucleus/metabolism , Sympathetic Nervous System/metabolism , Vasoconstrictor Agents/pharmacology , Vasodilator Agents/pharmacology
11.
Am J Physiol Heart Circ Physiol ; 294(1): H293-301, 2008 Jan.
Article in English | MEDLINE | ID: mdl-17982013

ABSTRACT

We previously developed a mathematical analysis technique for estimating the static gain values of the arterial total peripheral resistance (TPR) baroreflex (G(A)) and the cardiopulmonary TPR baroreflex (G(C)) from small, spontaneous beat-to-beat fluctuations in arterial blood pressure, cardiac output, and stroke volume. Here, we extended the mathematical analysis so as to also estimate the entire arterial TPR baroreflex impulse response [h(A)(t)] as well as the lumped arterial compliance (AC). The extended technique may therefore provide a linear dynamic characterization of TPR baroreflex systems during normal physiological conditions from potentially noninvasive measurements. We theoretically evaluated the technique with respect to realistic spontaneous hemodynamic variability generated by a cardiovascular simulator with known system properties. Our results showed that the technique reliably estimated h(A)(t) [error = 30.2 +/- 2.6% for the square root of energy (E(A)), 19.7 +/- 1.6% for absolute peak amplitude (P(A)), 37.3 +/- 2.5% for G(A), and 33.1 +/- 4.9% for the overall time constant] and AC (error = 17.6 +/- 4.2%) under various simulator parameter values and reliably tracked changes in G(C). We also experimentally evaluated the technique with respect to spontaneous hemodynamic variability measured from seven conscious dogs before and after chronic arterial baroreceptor denervation. Our results showed that the technique correctly predicted the abolishment of h(A)(t) [E(A) = 1.0 +/- 0.2 to 0.3 +/- 0.1, P(A) = 0.3 +/- 0.1 to 0.1 +/- 0.0 s(-1), and G(A) = -2.1 +/- 0.6 to 0.3 +/- 0.2 (P < 0.05)] and the enhancement of G(C) [-0.7 +/- 0.44 to -1.8 +/- 0.2 (P < 0.05)] following the chronic intervention. Moreover, the technique yielded estimates whose values were consistent with those reported with more invasive and/or experimentally difficult methods.


Subject(s)
Baroreflex , Computer Simulation , Hemodynamics , Models, Cardiovascular , Physical Exertion/physiology , Pressoreceptors/physiology , Vascular Resistance , Animals , Arteries/physiology , Blood Pressure , Cardiac Output , Compliance , Denervation , Dogs , Heart Rate , Linear Models , Reproducibility of Results , Time Factors
12.
Article in English | MEDLINE | ID: mdl-18002141

ABSTRACT

We have previously proposed a technique for estimating the static gain values and impulse response of the arterial and cardiopulmonary total peripheral resistance (TPR) baroreflex by mathematical analysis of beat-to-beat fluctuations in arterial blood pressure, cardiac output, and stroke volume. In this study, we evaluated the technique with respect to spontaneous hemodynamic variability measured from seven conscious dogs before and after chronic arterial baroreceptor denervation. Our results show that the technique correctly predicted the alterations in TPR baroreflex functioning that are known to occur following the baroreceptor denervation.


Subject(s)
Baroreflex/physiology , Blood Pressure/physiology , Models, Cardiovascular , Pressoreceptors/physiology , Vascular Resistance/physiology , Algorithms , Animals , Computer Simulation , Denervation/methods , Dogs
13.
Am J Physiol Heart Circ Physiol ; 291(5): H2453-61, 2006 Nov.
Article in English | MEDLINE | ID: mdl-16751294

ABSTRACT

Stimulation of nucleus of the solitary tract (NTS) A(2a)-adenosine receptors elicits cardiovascular responses quite similar to those observed with rapid, severe hemorrhage, including bradycardia, hypotension, and inhibition of renal but activation of preganglionic adrenal sympathetic nerve activity (RSNA and pre-ASNA, respectively). Because adenosine levels in the central nervous system increase during severe hemorrhage, we investigated to what extent these responses to hemorrhage may be due to activation of NTS adenosine receptors. In urethane- and alpha-chloralose-anesthetized male Sprague-Dawley rats, rapid hemorrhage was performed before and after bilateral nonselective or selective blockade of NTS adenosine-receptor subtypes [A(1)- and A(2a)-adenosine-receptor antagonist 8-(p-sulfophenyl)theophylline (1 nmol/100 nl) and A(2a)-receptor antagonist ZM-241385 (40 pmol/100 nl)]. The nonselective blockade reversed the response in RSNA (-21.0 +/- 9.6 Delta% vs. +7.3 +/- 5.7 Delta%) (where Delta% is averaged percent change from baseline) and attenuated the average heart rate response (change of -14.8 +/- 4.8 vs. -4.4 +/- 3.4 beats/min). The selective blockade attenuated the RSNA response (-30.4 +/- 5.2 Delta% vs. -11.1 +/- 7.7 Delta%) and tended to attenuate heart rate response (change of -27.5 +/- 5.3 vs. -15.8 +/- 8.2 beats/min). Microinjection of vehicle (100 nl) had no significant effect on the responses. The hemorrhage-induced increases in pre-ASNA remained unchanged with either adenosine-receptor antagonist. We conclude that adenosine operating in the NTS via A(2a) and possibly A(1) receptors may contribute to posthemorrhagic sympathoinhibition of RSNA but not to the sympathoactivation of pre-ASNA. The differential effects of NTS adenosine receptors on RSNA vs. pre-ASNA responses to hemorrhage supports the hypothesis that these receptors are differentially located/expressed on NTS neurons/synaptic terminals controlling different sympathetic outputs.


Subject(s)
Blood Pressure/physiology , Hemorrhage/physiopathology , Kidney/innervation , Neural Inhibition/physiology , Receptors, Purinergic P1/metabolism , Solitary Nucleus/physiology , Sympathetic Nervous System/physiology , Anesthesia , Animals , Blood Pressure/drug effects , Heart Rate/drug effects , Hemorrhage/etiology , Male , Microinjections , Purinergic P1 Receptor Antagonists , Rats , Rats, Sprague-Dawley , Solitary Nucleus/drug effects , Sympathetic Nervous System/drug effects , Theophylline/analogs & derivatives , Theophylline/pharmacology , Triazines/pharmacology , Triazoles/pharmacology
14.
Am J Physiol Heart Circ Physiol ; 290(2): H807-12, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16199474

ABSTRACT

We have previously shown that activation of P2X purinoceptors in the subpostremal nucleus tractus solitarius (NTS) produces a rapid bradycardia and hypotension. This bradycardia could occur via sympathetic withdrawal, parasympathetic activation, or a combination of both mechanisms. Thus we investigated the relative roles of parasympathetic activation and sympathetic withdrawal in mediating this bradycardia in chloralose-urethane anesthetized male Sprague-Dawley rats. Microinjections of the selective P2X purinoceptor agonist alpha,beta-methylene ATP (25 pmol/50 nl and 100 pmol/50 nl) were made into the subpostremal NTS in control animals, after atenolol (2 mg/kg i.v.), a beta1-selective antagonist, and after atropine methyl bromide (2 mg/kg i.v.), a muscarinic receptor antagonist. The bradycardia observed with activation of P2X receptors at the low dose of the agonist is mediated almost entirely by sympathetic withdrawal. After beta1-adrenergic blockade, the bradycardia was reduced to just -5.1 +/- 0.5 versus -28.8 +/- 5.1 beats/min in intact animals. Muscarinic blockade did not produce any significant change in the bradycardic response at the low dose. At the high dose, both beta1-adrenergic blockade and muscarinic blockade attenuated the bradycardia similarly, -37.4 +/- 6.4 and -40.6 +/- 3.7 beats/min, respectively, compared with -88.0 +/- 11 beats/min in control animals. Double blockade of both beta1-adrenergic and muscarinic receptors virtually abolished the response (-2.5 +/- 0.8 beats/min). We conclude that the relative contributions of parasympathetic activation and sympathetic withdrawal are dependent on the extent of P2X receptor activation.


Subject(s)
Bradycardia/physiopathology , Parasympathetic Nervous System/physiopathology , Receptors, Purinergic P2/metabolism , Solitary Nucleus/metabolism , Sympathetic Nervous System/physiopathology , Adenosine Triphosphate/administration & dosage , Adenosine Triphosphate/analogs & derivatives , Adenosine Triphosphate/pharmacology , Adrenergic beta-Antagonists/administration & dosage , Adrenergic beta-Antagonists/pharmacology , Animals , Atenolol/administration & dosage , Atenolol/pharmacology , Atropine Derivatives/administration & dosage , Atropine Derivatives/pharmacology , Injections, Intravenous , Male , Microinjections , Muscarinic Antagonists/administration & dosage , Muscarinic Antagonists/pharmacology , Purinergic P2 Receptor Agonists , Rats , Rats, Sprague-Dawley , Receptors, Purinergic P2X , Solitary Nucleus/drug effects
15.
Am J Physiol Heart Circ Physiol ; 290(5): H1889-98, 2006 May.
Article in English | MEDLINE | ID: mdl-16339828

ABSTRACT

Activation of adenosine A2a receptors in the nucleus of the solitary tract (NTS) decreases mean arterial pressure (MAP), heart rate (HR), and renal sympathetic nerve activity (RSNA), whereas increases in preganglionic adrenal sympathetic nerve activity (pre-ASNA) occur, a pattern similar to that observed during hypotensive hemorrhage. Central vasopressin V1 receptors may contribute to posthemorrhagic hypotension and bradycardia. Both V1 and A2a receptors are densely expressed in the NTS, and both of these receptors are involved in cardiovascular control; thus they may interact. The responses elicited by NTS A2a receptors are mediated mostly via nonglutamatergic mechanisms, possibly via release of vasopressin. Therefore, we investigated whether blockade of NTS V1 receptors alters the autonomic response patterns evoked by stimulation of NTS A2a receptors (CGS-21680, 20 pmol/50 nl) in alpha-chloralose-urethane anesthetized male Sprague-Dawley rats. In addition, we compared the regional sympathetic responses to microinjections of vasopressin (0.1-100 ng/50 nl) into the NTS. Blockade of V1 receptors reversed the normal decreases in MAP into increases (-95.6 +/- 28.3 vs. 51.4 +/- 15.7 integralDelta%), virtually abolished the decreases in HR (-258.3 +/- 54.0 vs. 18.9 +/- 57.8 integralDeltabeats/min) and RSNA (-239.3 +/- 47.4 vs. 15.9 +/- 36.1 integralDelta%), and did not affect the increases in pre-ASNA (279.7 +/- 48.3 vs. 233.1 +/- 54.1 integralDelta%) evoked by A2a receptor stimulation. The responses partially returned toward normal values approximately 90 min after the blockade. Microinjections of vasopressin into the NTS evoked dose-dependent decreases in HR and RSNA and variable MAP and pre-ASNA responses with a tendency toward increases. We conclude that the decreases in MAP, HR, and RSNA in response to NTS A2a receptor stimulation may be mediated via release of vasopressin from neural terminals in the NTS. The differential effects of NTS V1 and A2a receptors on RSNA versus pre-ASNA support the hypothesis that these receptor subtypes are differentially located/expressed on NTS neurons/neural terminals controlling different sympathetic outputs.


Subject(s)
Action Potentials/physiology , Blood Pressure/physiology , Neural Inhibition/physiology , Receptor, Adenosine A2A/metabolism , Receptors, Vasopressin/metabolism , Solitary Nucleus/physiology , Sympathetic Nervous System/physiology , Adenosine A2 Receptor Agonists , Animals , Feedback/physiology , Male , Rats , Rats, Sprague-Dawley
16.
Am J Physiol Heart Circ Physiol ; 290(5): H1830-6, 2006 May.
Article in English | MEDLINE | ID: mdl-16306216

ABSTRACT

Feedback control of total peripheral resistance (TPR) by the arterial and cardiopulmonary baroreflex systems is an important mechanism for short-term blood pressure regulation. Existing methods for measuring this TPR baroreflex mechanism typically aim to quantify only the gain value of one baroreflex system as it operates in open-loop conditions. As a result, the normal, integrated functioning of the arterial and cardiopulmonary baroreflex control of TPR remains to be fully elucidated. To this end, the laboratory of Mukkamala et al. (Mukkamala R, Toska K, and Cohen RJ. Am J Physiol Heart Circ Physiol 284: H947-H959, 2003) previously proposed a potentially noninvasive technique for estimating the closed-loop (dimensionless) gain values of the arterial TPR baroreflex (GA) and the cardiopulmonary TPR baroreflex (GC) by mathematical analysis of the subtle, beat-to-beat fluctuations in arterial blood pressure, cardiac output, and stroke volume. Here, we review the technique with additional details and describe its experimental evaluation with respect to spontaneous hemodynamic variability measured from seven conscious dogs, before and after chronic arterial baroreceptor denervation. The technique was able to correctly predict the group-average changes in GA and GC that have previously been shown to occur following chronic arterial baroreceptor denervation. That is, reflex control by the arterial TPR baroreflex was virtually abolished (GA = -2.1 +/- 0.6 to 0.3 +/- 0.2; P < 0.05), while reflex control by the cardiopulmonary TPR baroreflex more than doubled (GC = -0.7 +/- 0.4 to -1.8 +/- 0.2; P < 0.05). With further successful experimental testing, the technique may ultimately be employed to advance the basic understanding of TPR baroreflex functioning in both humans and animals in health and disease.


Subject(s)
Baroreflex/physiology , Blood Flow Velocity/physiology , Blood Pressure/physiology , Models, Cardiovascular , Pressoreceptors/physiology , Pulmonary Circulation/physiology , Vascular Resistance/physiology , Animals , Denervation , Dogs , Feedback/physiology
17.
Am J Physiol Heart Circ Physiol ; 289(6): H2536-42, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16100243

ABSTRACT

Our previous studies concluded that stimulation of the nucleus of the solitary tract (NTS) A2a receptors evokes preferential hindlimb vasodilation mainly via inducing increases in preganglionic sympathetic nerve activity (pre-ASNA) directed to the adrenal medulla. This increase in pre-ASNA causes the release of epinephrine and subsequent activation of beta-adrenergic receptors that are preferentially located in the skeletal muscle vasculature. Selective activation of NTS A1 adenosine receptors evokes variable, mostly pressor effects and increases pre-ASNA, as well as lumbar sympathetic activity, which is directed to the hindlimb. These counteracting factors may have opposite effects on the hindlimb vasculature resulting in mixed vascular responses. Therefore, in chloralose-urethane-anesthetized rats, we evaluated the contribution of vasodilator versus vasoconstrictor effects of stimulation of NTS A1 receptors on the hindlimb vasculature. We compared the changes in iliac vascular conductance evoked by microinejctions into the NTS of the selective A1 receptor agonist N6-cyclopentyladenosine (330 pmol in 50 nl volume) in intact animals with the responses evoked after beta-adrenergic blockade, bilateral adrenalectomy, bilateral lumbar sympathectomy, and combined adrenalectomy + lumbar sympathectomy. In intact animals, stimulation of NTS A1 receptors evoked variable effects: increases and decreases in mean arterial pressure and iliac conductance with prevailing pressor and vasoconstrictor effects. Peripheral beta-adrenergic receptor blockade and bilateral adrenalectomy eliminated the depressor component of the responses, markedly potentiated iliac vasoconstriction, and tended to increase the pressor responses. Lumbar sympathectomy tended to decrease the pressor and vasoconstrictor responses. After bilateral adrenalectomy plus lumbar sympathectomy, a marked vasoconstriction in iliac vascular bed still persisted, suggesting that the vasoconstrictor component of the response to stimulation of NTS A1 receptors is mediated mostly via circulating factors (e.g., vasopressin, angiotensin II, or circulating catecholamines released from other sympathetic terminals). These data strongly suggest that stimulation of NTS A1 receptors exerts counteracting effects on the iliac vascular bed: activation of the adrenal medulla and beta-adrenergic vasodilation versus vasoconstriction mediated by neural and humoral factors.


Subject(s)
Muscle, Skeletal/blood supply , Muscle, Skeletal/physiology , Receptor, Adenosine A1/metabolism , Receptors, Adrenergic, beta/metabolism , Solitary Nucleus/physiology , Vasodilation/physiology , Animals , Hindlimb/blood supply , Hindlimb/physiology , Male , Rats , Rats, Sprague-Dawley
18.
Am J Physiol Heart Circ Physiol ; 289(6): H2416-23, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16055513

ABSTRACT

Previous studies have shown that heart failure (HF) or sinoaortic denervation (SAD) alters the strength and mechanisms of the muscle metaboreflex during dynamic exercise. However, it is still unknown to what extent SAD may modify the muscle metaboreflex in HF. Therefore, we quantified the contribution of cardiac output (CO) and peripheral vasoconstriction to metaboreflex-mediated increases in mean arterial blood pressure (MAP) in conscious, chronically instrumented dogs before and after induction of HF in both barointact and SAD conditions during mild and moderate exercise. The muscle metaboreflex was activated via partial reductions in hindlimb blood flow. After SAD, the metaboreflex pressor responses were significantly higher with respect to the barointact condition despite lower CO responses. The pressor response was significantly lower in HF after SAD but still higher than that of HF in the barointact condition. During control experiments in the barointact condition, total vascular conductance summed from all beds except the hindlimbs did not change with muscle metaboreflex activation, whereas in the SAD condition both before and after induction of HF significant vasoconstriction occurred. We conclude that SAD substantially increased the contribution of peripheral vasoconstriction to metaboreflex-induced increases in MAP, whereas in HF SAD did not markedly alter the patterns of the reflex responses, likely reflecting that in HF the ability of the arterial baroreflex to buffer metaboreflex responses is impaired.


Subject(s)
Arteries/physiopathology , Baroreflex , Blood Pressure , Heart Failure/physiopathology , Heart Rate , Muscle, Skeletal/physiopathology , Physical Exertion , Adaptation, Physiological/physiology , Animals , Cardiac Output , Dogs , Female , Male
19.
Neurol Res ; 27(2): 182-94, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15829182

ABSTRACT

OBJECTIVES: This review addresses the role of central purinergic receptors in the operation of the cardiovascular reflexes. METHODS: Potential physiological role of purinergic receptors operating in the nucleus of the solitary tract (NTS) was assessed via comparison of the regional patterns of hemodynamic and sympathetic responses evoked by selective stimulation/inhibition of NTS purinergic receptor subtypes, with the patterns evoked by stimulation and unloading of arterial baroreceptors, and other known patterns of autonomic responses. The effects of sino-aortic denervation plus vagotomy and ionotropic glutamatergic blockade of NTS mechanisms on the patterns of the responses were also considered. RESULTS: Selective stimulation of NTS A1 receptors with CPA evoked a pattern of regional autonomic responses consistent with inhibition of baroreflex mechanisms and facilitation/ disinhibition of chemoreflex mechanisms. Selective stimulation of NTS A(2a) receptors with CGS 21680-evoked pattern of the responses different than that evoked by stimulation of baroreflex afferents what remains in contrast to previous reports suggesting that NTS A2a receptors facilitate baroreflex transmission. The pattern of the responses was similar to that observed during hypotensive hemorrhage. Preferential, b -adrenergic iliac vasodilation evoked by stimulation of adenosine A2a receptors and preferential activation of sympathetic output to the adrenal medulla by both adenosine A1 and A2a receptors are consistent with contribution of these receptors to the defense response, stress and exercise. These observations support previous findings that NTS A1 receptors contribute to the hypothalamic defense response. The effects of stimulation and blockade of NTS P2x receptors with alpha, beta-methylene ATP and suramin, respectively, suggested that neuronally-released ATP operating via P2x receptors may be a crucial co-transmitter with glutamate in mediating baroreflex responses. DISCUSSION: The above observations strongly suggest that purinergic receptor subtypes operating in NTS circuitry are linked to specific afferent and descending mechanisms primarily integrated in the NTS.


Subject(s)
Cardiovascular System/metabolism , Receptors, Purinergic/physiology , Solitary Nucleus/physiology , Adenosine/pharmacology , Adenosine/physiology , Adenosine Triphosphate/pharmacology , Adenosine Triphosphate/physiology , Animals , Baroreflex/drug effects , Baroreflex/physiology , Blood Pressure/drug effects , Blood Pressure/physiology , Heart Rate/drug effects , Neurotransmitter Agents/pharmacology , Neurotransmitter Agents/physiology , Receptors, Purinergic/classification , Receptors, Purinergic/drug effects , Solitary Nucleus/drug effects , Sympathetic Nervous System
20.
Am J Physiol Heart Circ Physiol ; 288(3): H1374-80, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15539416

ABSTRACT

Previous studies showed that the arterial baroreflex opposes the pressor response mediated by muscle metaboreflex activation during mild dynamic exercise. However, no studies have investigated the mechanisms contributing to metaboreflex-mediated pressor responses during dynamic exercise after arterial baroreceptor denervation. Therefore, we investigated the contribution of cardiac output (CO) and peripheral vasoconstriction in mediating the pressor response to graded reductions in hindlimb perfusion in conscious, chronically instrumented dogs before and after sinoaortic denervation (SAD) during mild and moderate exercise. In control experiments, the metaboreflex pressor responses were mediated via increases in CO. After SAD, the metaboreflex pressor responses were significantly greater and significantly smaller increases in CO occurred. During control experiments, nonischemic vascular conductance (NIVC) did not change with muscle metaboreflex activation, whereas after SAD NIVC significantly decreased with metaboreflex activation; thus SAD shifted the mechanisms of the muscle metaboreflex from mainly increases in CO to combined cardiac and peripheral vasoconstrictor responses. We conclude that the major mechanism by which the arterial baroreflex buffers the muscle metaboreflex is inhibition of metaboreflex-mediated peripheral vasoconstriction.


Subject(s)
Aorta/physiology , Baroreflex/physiology , Cardiac Output/physiology , Physical Exertion/physiology , Animals , Aorta/innervation , Blood Pressure/physiology , Denervation , Dogs , Female , Male , Vasoconstriction/physiology
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