ABSTRACT
Pleomorphic adenoma or mixed tumour (MT) is a benign neoplasia of slow growth and epithelial histogenesis. We report a particular case of recurring MT of the hard palate. A 39-year-old man came to us with a swelling of the hard half-palate. The patient, 19 years earlier, had had a small formation in the same place that, over a period of three years had slowly grown. Histology showed that it was an MT and it was promptly removed. Sixteen years after the operation, a small recurrence reappeared, reaching a diameter of 12 mm. The patient underwent a new excision. The case reported is of particular interest due to many aspects: the outbreak from the minor salivary glands; the male sex; the young age of the patient at the first sign of the tumour; the appearance of a recurrence after 16 years, not contemplated in literature; and finally, the rapid growth of the second appearance.
Subject(s)
Adenoma, Pleomorphic/surgery , Neoplasm Recurrence, Local/surgery , Palatal Neoplasms/surgery , Salivary Gland Neoplasms/surgery , Adenoma, Pleomorphic/pathology , Adult , Humans , Male , Neoplasm Recurrence, Local/pathology , Palatal Neoplasms/pathology , Palate, Hard/pathology , Palate, Hard/surgery , Salivary Gland Neoplasms/pathology , Salivary Glands, Minor , Time FactorsABSTRACT
The roughness and the purity of implant surfaces are key points in the osteointegration process. The surfaces obtained by classic methods present irregular non-reproducible patterns and furthermore contaminate the implant surface with materials other than titanium which interfere with the process of osteointegration. The aim of the present study is to evaluate, by SEM/EDX and XPS analyses, the surface microstructure and the purity of new laser-treated implant surfaces. The laser treatment of the surface allows to set parameters to determine the roughness in order to obtain a regular and repeatable surface. Furthermore, there being no contact between the implant and the machine, there is no surface contamination with elements other than titanium. In this study we used a diode-pumped solid state laser (DPSS) with Nd:YAG source operating in Q-Switching mode on titanium samples. The resulting samples were analysed by SEM/EDX and XPS to evaluate morphology and purity of the surface. The results show surfaces with very regular roughness and a total absence of contamination.
Subject(s)
Lasers , Microscopy, Electron, Scanning/methods , Prosthesis Implantation/methods , Spectrum Analysis/methods , Surface PropertiesABSTRACT
El objetivo de este trabajo es aumentar la sensibilidad de la detección de antígeno de VIH en pacientes infectados a través de un proceso de disociación de complejos inmunes que no reduzca la reactividad antigénica. Se realizó una etapa de precipitación con PEG 12 por ciento (concentración final 2 por ciento), tratamiento ácido disociante y neutralización, previo a la aplicación de un método convencional de captura de antígeno. Se mostró una relación significativa entre el tratamiento con PEG disociante y la detección de antígeno (X2: 13,97, p < 0,001). De 105 muestras, 27 sueros que resultaron negativos por el método estándar fueron positivos con el tratamiento. La cantidad de antígeno en 35 muestras previamente positivas se incrementó en promedio 2,3 veces luego del tratamiento
Subject(s)
Humans , HIV Antigens/isolation & purification , Antigen-Antibody Complex/immunology , Polyethylene Glycols , Sensitivity and Specificity , Acquired Immunodeficiency Syndrome/diagnosis , ArgentinaABSTRACT
El objetivo de este trabajo es aumentar la sensibilidad de la detección de antígeno de VIH en pacientes infectados a través de un proceso de disociación de complejos inmunes que no reduzca la reactividad antigénica. Se realizó una etapa de precipitación con PEG 12 por ciento (concentración final 2 por ciento), tratamiento ácido disociante y neutralización, previo a la aplicación de un método convencional de captura de antígeno. Se mostró una relación significativa entre el tratamiento con PEG disociante y la detección de antígeno (X2: 13,97, p < 0,001). De 105 muestras, 27 sueros que resultaron negativos por el método estándar fueron positivos con el tratamiento. La cantidad de antígeno en 35 muestras previamente positivas se incrementó en promedio 2,3 veces luego del tratamiento (AU)
Subject(s)
Humans , HIV Antigens/isolation & purification , Acquired Immunodeficiency Syndrome/diagnosis , Antigen-Antibody Complex/immunology , Polyethylene Glycols/diagnosis , Sensitivity and Specificity , ArgentinaABSTRACT
The aim of this work was to increase sensitivity in the detection of antigens from HIV-infected patients, through a process of immune complex dissociation without loss of antigenicity. 500 microliters of sera were mixed with 100 microliters of PEG 12%, stored one night in refrigerator, and centrifuged at 2000 g during 20 minutes. 200 microliters of buffer AcH/Ac- (pH 3.5) were added to the sediment, and incubated at 37 degrees C during one hour with periodic shaking. This was neutralized with 100 microliters of buffer TRIS/CIH (pH 8.6). The antigen was investigated in the original sample, supernatant and sediment. Samples of 105 patients with positive serology, confirmed by Western Blot following CDC criteria, were processed. The antigen was detected in 62 (59%) samples precipitated with PEG, but only 35 (33%) when conventional methods were used. Applying statistics X2: 13.97, P < 0.001, a highly significant association can be observed between PEG dissociation treatment and antigen detection. 27 negative sera by the standard method became positive in the whole sediment, and only 8 in the supernatant. In addition, 40 negative sera were processed, which had not become positive for the antigen by PEG treatment.
Subject(s)
Antigen-Antibody Complex/immunology , HIV Antigens/blood , HIV-1/immunology , Chemical Precipitation , False Negative Reactions , HIV Infections/blood , HIV Infections/immunology , HIV Seronegativity , HIV Seropositivity/blood , Humans , Polyethylene Glycols , Reference Standards , Sensitivity and SpecificityABSTRACT
The aim of this work was to increase sensitivity in the detection of antigens from HIV-infected patients, through a process of immune complex dissociation without loss of antigenicity. 500 microliters of sera were mixed with 100 microliters of PEG 12
, stored one night in refrigerator, and centrifuged at 2000 g during 20 minutes. 200 microliters of buffer AcH/Ac- (pH 3.5) were added to the sediment, and incubated at 37 degrees C during one hour with periodic shaking. This was neutralized with 100 microliters of buffer TRIS/CIH (pH 8.6). The antigen was investigated in the original sample, supernatant and sediment. Samples of 105 patients with positive serology, confirmed by Western Blot following CDC criteria, were processed. The antigen was detected in 62 (59
) samples precipitated with PEG, but only 35 (33
) when conventional methods were used. Applying statistics X2: 13.97, P < 0.001, a highly significant association can be observed between PEG dissociation treatment and antigen detection. 27 negative sera by the standard method became positive in the whole sediment, and only 8 in the supernatant. In addition, 40 negative sera were processed, which had not become positive for the antigen by PEG treatment.
