Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 26
Filter
Add more filters










Publication year range
1.
Mol Oncol ; 8(1): 142-9, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24210755

ABSTRACT

PURPOSE: Operable thoracic esophageal/gastroesophageal junction carcinoma (EC) is often treated with chemoradiation and surgery but tumor responses are unpredictable and heterogeneous. We hypothesized that aldehyde dehydrogenase-1 (ALDH-1) could be associated with response. METHODS: The labeling indices (LIs) of ALDH-1 by immunohistochemistry in untreated tumor specimens were established in EC patients who had chemoradiation and surgery. Univariate logistic regression and 3-fold cross validation were carried out for the training (67% of patients) and validation (33%) sets. Non-clinical experiments in EC cells were performed to generate complimentary data. RESULTS: Of 167 EC patients analyzed, 40 (24%) had a pathologic complete response (pathCR) and 27 (16%) had an extremely resistant (exCRTR) cancer. The median ALDH-1 LI was 0.2 (range, 0.01-0.85). There was a significant association between pathCR and low ALDH-1 LI (p ≤ 0.001; odds-ratio [OR] = 0.432). The 3-fold cross validation led to a concordance index (C-index) of 0.798 for the fitted model. There was a significant association between exCRTR and high ALDH-1 LI (p ≤ 0.001; OR = 3.782). The 3-fold cross validation led to the C-index of 0.960 for the fitted model. In several cell lines, higher ALDH-1 LIs correlated with resistant/aggressive phenotype. Cells with induced chemotherapy resistance upregulated ALDH-1 and resistance conferring genes (SOX9 and YAP1). Sorted ALDH-1+ cells were more resistant and had an aggressive phenotype in tumor spheres than ALDH-1- cells. CONCLUSIONS: Our clinical and non-clinical data demonstrate that ALDH-1 LIs are predictive of response to therapy and further research could lead to individualized therapeutic strategies and novel therapeutic targets for EC patients.


Subject(s)
Adenocarcinoma/pathology , Adenocarcinoma/therapy , Esophageal Neoplasms/pathology , Esophageal Neoplasms/therapy , Esophagus/pathology , Isoenzymes/analysis , Retinal Dehydrogenase/analysis , Adenocarcinoma/diagnosis , Adenocarcinoma/genetics , Adult , Aged , Aged, 80 and over , Aldehyde Dehydrogenase 1 Family , Cell Line, Tumor , Chemoradiotherapy , Esophageal Neoplasms/diagnosis , Esophageal Neoplasms/genetics , Esophagus/drug effects , Esophagus/metabolism , Esophagus/radiation effects , Female , Gene Expression Regulation, Neoplastic , Humans , Immunohistochemistry , Isoenzymes/genetics , Male , Middle Aged , Prognosis , Retinal Dehydrogenase/genetics
2.
Eye (Lond) ; 24(3): 491-6, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20019765

ABSTRACT

Much debate exists within the literature with respect to the variant of neovascular age-related macular degeneration (AMD) known as retinal angiomatous proliferation (RAP). We prefer the term retinal anastomosis to the lesion (RAL), as we believe that the choroidal neovascularization (CNV) lesion precedes the development of an anastomosing retinal vessel to this lesion. Natural history data surrounding RAP lesions are available through analysis of the eyes with subfoveal occult CNV within the placebo arm of the Verteporfin in Photodynamic Therapy (VIP) trial. Although many reports ascribe a poor prognosis to RAP lesions, the data suggest that the true natural history of RAP lesion may be highly variable and similar to that of other CNV lesions. Information from clinical trials suggests that the response of RAP lesions to CNV treatments may also be similar to that of other variants of neovascular AMD.


Subject(s)
Angiomatosis/pathology , Retinal Neovascularization/pathology , Choroid/blood supply , Humans , Macular Degeneration/pathology
3.
Phys Rev Lett ; 102(1): 011801, 2009 Jan 09.
Article in English | MEDLINE | ID: mdl-19257180

ABSTRACT

Using 2.45x10;{7} psi(2S) decays collected with the CLEO-c detector at the Cornell Electron Storage Ring we present the most precise measurements of magnetic dipole transitions in the charmonium system. We measure B(psi(2S)-->gammaeta_{c})=(4.32+/-0.16+/-0.60)x10;{-3}, B(J/psi-->gammaeta_{c})/B(psi(2S)-->gammaeta_{c})=4.59+/-0.23+/-0.64, and B(J/psi-->gammaeta_{c})=(1.98+/-0.09+/-0.30)%. We observe a distortion in the eta_{c} line shape due to the photon-energy dependence of the magnetic dipole transition rate. We find that measurements of the eta_{c} mass are sensitive to the line shape, suggesting an explanation for the discrepancy between measurements of the eta_{c} mass in radiative transitions and other production mechanisms.

4.
Phys Rev Lett ; 102(8): 081801, 2009 Feb 27.
Article in English | MEDLINE | ID: mdl-19257732

ABSTRACT

Using a 281 pb-1 data sample collected at the psi(3770) resonance with the CLEO-c detector at the Cornell Electron Storage Ring, we report the first observation of D+ --> etae + nue. We also set upper limits for D+ --> eta'e + nue and D + --> varphie + nue that are about 2 orders of magnitude more restrictive than those obtained by previous experiments.

6.
Phys Rev Lett ; 101(15): 151801, 2008 Oct 10.
Article in English | MEDLINE | ID: mdl-18999588

ABSTRACT

Using a total of 2.74 x 10(7) decays of the psi(2S) collected with the CLEO-c detector, we present a study of chi(cJ)-->gammaV, where V=rho(0), omega, phi. The transitions chi(c1)-->gammarho(0 and chi(c1)-->gammaomega are observed with B(chi(c1)-->gammarho(0))=(2.43+/-0.19+/-0.22) x 10(-4) and B(chi(c1)-->gammaomega)=(8.3+/-1.5+/-1.2) x 10(-5). In the chi(c1)-->gammarho(0) transition, the final state meson is dominantly longitudinally polarized. Upper limits on the branching fractions of other chi(cJ) states to light vector mesons are presented.

7.
Phys Rev Lett ; 100(25): 251802, 2008 Jun 27.
Article in English | MEDLINE | ID: mdl-18643653

ABSTRACT

By using 1.8x10{6} DDpairs, we have measured B(D{0}-->pi{-}e{+}nu{e})=0.299(11)(9)%, B(D{+}-->pi{0}e{+}nu{e})=0.373(22)(13)%, B(D{0}-->K{-}e{+}nu{e})=3.56(3)(9)%, and B(D{+}-->K{0}e{+}nu{e})=8.53(13)(23)% and have studied the q;{2} dependence of the form factors. By combining our results with recent lattice calculations, we obtain |V{cd}|=0.217(9)(4)(23) and |V{cs}|=1.015(10)(11)(106).

8.
Phys Rev Lett ; 100(18): 181802, 2008 May 09.
Article in English | MEDLINE | ID: mdl-18518362

ABSTRACT

Using e+e--->Ds*-Ds+ data collected near the peak Ds production energy, Ecm=4170 MeV, with the CLEO-c detector, we present the first observation of the decay Ds+-->pn. We measure a branching fraction B(Ds+-->pn)=(1.30+/-0.36(-0.16)+0.12)x10(-3). This is the first observation of a charmed meson decaying into a baryon-antibaryon final state.

9.
Phys Rev Lett ; 98(5): 052002, 2007 Feb 02.
Article in English | MEDLINE | ID: mdl-17358847

ABSTRACT

Using data collected with the CLEO III detector at the CESR e+e- collider, we report on a first observation of the decay Upsilon(3S)-->tau+tau-, and precisely measure the ratio of branching fractions of Upsilon(nS), n=1, 2, 3, to tau+tau- and mu+mu- final states, finding agreement with expectations from lepton universality. We derive absolute branching fractions for these decays, and also set a limit on the influence of a low mass CP-odd Higgs boson in the decay of the Upsilon(1S).

10.
Phys Rev Lett ; 98(9): 092002, 2007 Mar 02.
Article in English | MEDLINE | ID: mdl-17359150

ABSTRACT

A precision measurement of the D0 meson mass has been made using approximately 281 pb(-1) of e+e- annihilation data taken with the CLEO-c detector at the psi(3770) resonance. The exclusive decay D0-->K_{S}phi has been used to obtain M(D0)=1864.847+/-0.150(stat)+/-0.095(syst) MeV. This corresponds to M(D0D*0)=3871.81+/-0.36 MeV, and leads to a well-constrained determination of the binding energy of the proposed D0D*0 molecule X(3872), as Eb=0.6+/-0.6 MeV.

11.
Phys Rev Lett ; 96(8): 081802, 2006 Mar 03.
Article in English | MEDLINE | ID: mdl-16606168

ABSTRACT

Using of data collected with the CLEO-c detector, we report on first observations and measurements of Cabibbo-suppressed decays of D mesons in the following six decay modes: pi+ pi- pi0 pi0, pi+ pi+ pi- pi- pi0, pi+ pi0 pi0, pi+ pi+ pi- pi0, eta pi0, and omega pi+ pi-. Improved branching fraction measurements in eight other multipion decay modes are also presented. The measured D --> pi pi rates allow us to extract the ratio of isospin amplitudes A(DeltaI = (3/2) / A(DeltaI = (1/2)) = 0.420 +/- 0.014(stat) +/- 0.016(syst) and the strong phase shift of delta1 = (86.4 +/- 2.8 +/- 3.3) degrees, which is quite large and now more precisely determined.

12.
Phys Rev Lett ; 96(9): 092002, 2006 Mar 10.
Article in English | MEDLINE | ID: mdl-16606255

ABSTRACT

We measure the cross section for e+e- -->psi(3770) -->hadrons at Ec.m.=3773 MeV to be (6.38+/-0.08(+0.41)(-0.30) nb using the CLEO detector at the CESR e+e- collider. The difference between this and the e+e- -->psi(3770) -->DD cross section at the same energy is found to be (-0.01+/-0.08(+0.41)(-0.30) nb. With the observed total cross section, we extract Gamma(ee)(psi(3770))=(0.204+/-0.003(+0.041)(-0.027) keV. Uncertainties shown are statistical and systematic, respectively.

13.
J Econ Entomol ; 98(3): 796-804, 2005 Jun.
Article in English | MEDLINE | ID: mdl-16022308

ABSTRACT

The effects of planting dates 2-3-wk apart on boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), field-level populations, and feeding and oviposition damage to cotton, Gossypium hirsutum L., squares and bolls, were studied during 2002 and 2003 in the Lower Rio Grande Valley of Texas. Squares were 44-56% more abundant in some later planted treatments than in the earlier planted treatments, but mean cumulative numbers of oviposition- and feeding-damaged squares were 2.7 - 4.8-fold greater in some later planted treatments than in earlier treatments. Increased square production in later planted cotton was offset by boll weevil infestations that occurred when squares are most vulnerable and contribute most toward the pest's reproduction. Early planting avoided boll weevil population buildups in the field when large squares were abundant. Lint yields in 2002 did not differ significantly between the planting date treatments, but in 2003, mean yield in the middle treatment was 23% greater than in the early and late-planted treatments. Insecticide sprays in the earliest planted treatment of each year, based on the 10% damaged squares threshold, were >33% and >43% fewer than in the corresponding middle and latest planting treatments, respectively. Delayed planting, relative to the onset of favorable cotton-growing weather, at the field level, even when not applied uniformly on an areawide scale, is more cost-effective than planting too early or too late.


Subject(s)
Agriculture/methods , Gossypium/growth & development , Weevils/physiology , Animals , Climate , Fruit/growth & development , Insecticides/administration & dosage , Population Density , Reproduction , Time Factors
14.
Phys Rev Lett ; 95(26): 261803, 2005 Dec 31.
Article in English | MEDLINE | ID: mdl-16486342

ABSTRACT

Using 20.7 pb(-1) of e(+)e(-) annihilation data taken at sq.rt(r) = 3.671 GeV with the CLEO-c detector, precision measurements of the electromagnetic form factors of the charged pion, charged kaon, and proton have been made for timelike momentum transfer of |Q(2)| = 13.48 GeV(2) by the reaction e(+)e(-) --> h(+)h(-). The measurements are the first ever with identified pions and kaons of |Q(2)| > 4 GeV(2), with the results F(13.48 GeV(2)) = 0.075 +/- 0.008(stat) +/- 0.005(syst) and F(K)(13.48 GeV(2)) = 0.063 +/- 0.004(stat) +/- 0.001(syst). The result for the proton, assuming G(p)(E) = G(p)(M), is G(p)(M)(13.48 GeV(2)) = 0.014 +/- 0.002(stat) +/- 0.001(syst), which is in agreement with earlier results.

15.
Phys Rev Lett ; 93(11): 111801, 2004 Sep 10.
Article in English | MEDLINE | ID: mdl-15447329

ABSTRACT

Using 9.0 fb(-1) of integrated luminosity in e(+)e(-) collisions near the Upsilon(4S) mass collected with the CLEO II.V detector we report the first observation of the decay D0-->K(0)(S)etapi(0). We measure the ratio of branching fractions, BR(D0-->K(0)(S)etapi(0))BR / (D0-->K(0)(S)pi(0))=0.46+/-0.07+/-0.06. We perform a Dalitz analysis of 155 selected D0-->K(0)(S)etapi(0) candidates and find leading contributions from a(0)(980)K(0)(S) and K(*)(892)eta intermediate states.

16.
Eur J Pharmacol ; 420(2-3): 73-82, 2001 May 25.
Article in English | MEDLINE | ID: mdl-11408027

ABSTRACT

The baculovirus system has been used to express the rat dopamine D1 receptors in Spodoptera frugiperda (Sf9) cells. A panel of typical antipsychotics including, alpha-flupenthixol, fluphenazine and thioridizine were found to inhibit dopamine-dependent stimulation of adenylyl cyclase. However, these compounds were also found to inhibit adenylyl cyclase activity in the absence of agonist in Sf9 cells expressing dopamine D1-like receptors. Therefore, these nonselective dopamine receptor compounds displayed negative intrinsic or inverse agonist activity. None of the compounds tested were neutral antagonists.


Subject(s)
Antipsychotic Agents/pharmacology , Dopamine Agonists/pharmacology , Receptors, Dopamine D1/physiology , Adenylyl Cyclases/drug effects , Adenylyl Cyclases/metabolism , Animals , Baculoviridae/genetics , Binding, Competitive , Cell Line , Dopamine/pharmacology , Dose-Response Relationship, Drug , Gene Expression , Genetic Vectors/genetics , Ligands , Radioligand Assay , Rats , Receptors, Dopamine D1/agonists , Receptors, Dopamine D1/genetics , Receptors, Dopamine D5 , Spodoptera
17.
Brain Res Mol Brain Res ; 29(2): 347-57, 1995 Apr.
Article in English | MEDLINE | ID: mdl-7609622

ABSTRACT

Southern blot hybridization techniques have been used to identify genetic polymorphisms at the D2, D3 and D4 dopamine receptor loci in mice and rats. Genomic DNA from a panel of outbred and inbred strains of rats and inbred strains of mice was digested with a variety of restriction endonucleases. After separation of the restriction digests on the basis of size using agarose gel electrophoresis, 32P-labeled DNA probes coding for the rat D2, D3 and D4 dopamine receptors were used to identify a series of genetic polymorphisms at each of these receptor loci. Genetic polymorphisms were found for the rat and murine D2, D3 and D4 dopamine receptor loci. It is anticipated that these genetic polymorphisms will be useful in pharmacogenetic studies to determine the influence of the D2-like receptors in reward and addictive behaviors.


Subject(s)
Chromosome Mapping , Polymorphism, Genetic , Receptors, Dopamine D2/genetics , Animals , Genetic Code , Mice , Rats , Rats, Inbred Strains
18.
Drug Saf ; 5 Suppl 1: 95-100, 1990.
Article in English | MEDLINE | ID: mdl-2182070

ABSTRACT

Many drug products contain excipients which perform important functions in terms of stability, solubility and identification. Excipients should not be considered as inactive ingredients, as they have been associated with a wide range of adverse reactions in some individuals. Monitoring for excipient toxicity is important. Equally important, is the provision of non-medicinal ingredient information on packaging, patient information leaflets and prescribing information, as most excipient-related toxicity is preventable when the formulation is known.


Subject(s)
Excipients/toxicity , Animals , Drug Labeling , Humans , Legislation, Drug , United Kingdom
SELECTION OF CITATIONS
SEARCH DETAIL
...