ABSTRACT
A synthetic methodology for developing a polymeric nanoparticle for targeted computed tomographic (CT) imaging is revealed in this manuscript. The work describes a new class of soft type, vascularly constrained, stable colloidal radio-opaque metal-entrapped polymeric nanoparticle using organically soluble radio-opaque elements encapsulated by synthetic amphiphile. This agent offers several-fold CT signal enhancement in vitro and in vivo demonstrating detection sensitivity reaching to the low nanomolar particulate concentration range.
Subject(s)
Metal Nanoparticles/chemistry , Polymers/chemistry , Animals , Colloids , Half-Life , Metal Nanoparticles/analysis , Metal Nanoparticles/ultrastructure , Microscopy, Atomic Force , Microscopy, Electron, Scanning , Microscopy, Electron, Transmission , Molecular Structure , Rats , Tomography, X-Ray ComputedABSTRACT
In this work, we report novel fibrin targeted "soft-type" manganese-based contrast agents for MRI with the potential to noninvasively image intravascular thrombus which could warrant aggressive medical intervention to preclude subsequent myocardial infarction or stroke.
Subject(s)
Fibrin/metabolism , Manganese/chemistry , Manganese/metabolism , Nanostructures/chemistry , Thrombosis/diagnosis , Colloids , Contrast Media/chemistry , Contrast Media/metabolism , Magnetic Resonance Imaging , Manganese Compounds/chemistry , Manganese Compounds/metabolism , Oleic Acid/chemistry , Oleic Acid/metabolism , Oxides/chemistry , Oxides/metabolism , Sensitivity and Specificity , Substrate SpecificityABSTRACT
Spotting clots: Vascularly constrained colloidal gold nanobeacons (GNBs; see picture) can be used as exogenous photoacoustic contrast agents for the targeted detection of fibrin, a major biochemical feature of thrombus. Fibrin-targeted GNBs provide a more than tenfold signal enhancement in photoacoustic tomography in the near-IR wavelength window, indicating their potential for diagnostic imaging.
Subject(s)
Contrast Media/chemistry , Gold/chemistry , Metal Nanoparticles/chemistry , Tomography, Optical/methods , Ultrasonography/methods , Colloids , Fibrin/chemistry , Humans , Vascular Diseases/diagnosisABSTRACT
Although gadolinium has been the dominant paramagnetic metal for MR paramagnetic contrast agents, the recent association of this lanthanide with nephrogenic systemic fibrosis, an untreatable disease, has spawned renewed interest in alternative metals for MR molecular imaging. We have developed a self-assembled, manganese(III)-labeled nanobialys (1), a toroidal-shaped MR theranostic nanoparticle. In this report, Mn(III) nanobialys are characterized as MR molecular imaging agents for targeted detection of fibrin, a major biochemical feature of thrombus. A complementary ability of nanobialys to incorporate chemotherapeutic compounds with greater than 98% efficiency and to retain more than 80% of these drugs after infinite sink dissolution, point to the theranostic potential of this platform technology.
