ABSTRACT
Metastatic melanoma of unknown primary site accounts for approximately 5-10% of patients with metastatic melanoma. Various reasons have been suggested for the primary being unknown. These include a hidden location, complete regression of the primary or a primary origin of melanoma in lymph nodes. 24 cases with metastatic melanoma with primary of unknown site are presented. The characteristics and follow-up of the patients are discussed.
Subject(s)
Melanoma/secondary , Neoplasms, Unknown Primary/pathology , Skin Neoplasms/pathology , Aged , Female , Humans , Male , Middle Aged , Neoplasm Regression, Spontaneous/pathology , Neoplasms, Unknown Primary/diagnosis , Positron-Emission TomographyABSTRACT
Nine hybridoma cell lines secreting monoclonal antibodies specific for Nematospiroides dubius were produced by fusion of the mouse myeloma cell line NS-1 to either spleen cells or mesenteric lymph node cells from mice repeatedly infected with N. dubius. Seven of the antibodies were identified as IgM and two as IgG1. Each monoclonal antibody bound to polypeptide epitopes on both infective larvae (L3) and adult worms. However, five antibodies bound preferentially to L3 and three to adult worms. All nine antibodies reacted with high molecular weight protein antigens. Passive protective immunity in Balb/c mice was demonstrated with monoclonal antibodies Nd2 and Nd3 in ascites fluid which stunted both male and female worms and reduced parasite fecundity.
