Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
Diabetes ; 69(8): 1723-1734, 2020 08.
Article in English | MEDLINE | ID: mdl-32457219

ABSTRACT

The ß-cell protein synthetic machinery is dedicated to the production of mature insulin, which requires the proper folding and trafficking of its precursor, proinsulin. The complete network of proteins that mediate proinsulin folding and advancement through the secretory pathway, however, remains poorly defined. Here we used affinity purification and mass spectrometry to identify, for the first time, the proinsulin biosynthetic interaction network in human islets. Stringent analysis established a central node of proinsulin interactions with endoplasmic reticulum (ER) folding factors, including chaperones and oxidoreductases, that is remarkably conserved in both sexes and across three ethnicities. The ER-localized peroxiredoxin PRDX4 was identified as a prominent proinsulin-interacting protein. In ß-cells, gene silencing of PRDX4 rendered proinsulin susceptible to misfolding, particularly in response to oxidative stress, while exogenous PRDX4 improved proinsulin folding. Moreover, proinsulin misfolding induced by oxidative stress or high glucose was accompanied by sulfonylation of PRDX4, a modification known to inactivate peroxiredoxins. Notably, islets from patients with type 2 diabetes (T2D) exhibited significantly higher levels of sulfonylated PRDX4 than islets from healthy individuals. In conclusion, we have generated the first reference map of the human proinsulin interactome to identify critical factors controlling insulin biosynthesis, ß-cell function, and T2D.


Subject(s)
Diabetes Mellitus, Type 2/metabolism , Insulin/metabolism , Peroxiredoxins/metabolism , Proinsulin/chemistry , Proinsulin/metabolism , Blotting, Western , Diabetes Mellitus, Type 2/genetics , Endoplasmic Reticulum/genetics , Endoplasmic Reticulum/metabolism , Female , Humans , Immunoprecipitation , Insulin/chemistry , Male , Peroxiredoxins/genetics , Protein Binding , Protein Folding , Tandem Mass Spectrometry
2.
Cell Mol Gastroenterol Hepatol ; 6(2): 181-198, 2018.
Article in English | MEDLINE | ID: mdl-30003124

ABSTRACT

BACKGROUND & AIMS: Oncogenic mutations in KRAS, coupled with inactivation of p53, CDKN2A/p16INK4A, and SMAD4, drive progression of pancreatic ductal adenocarcinoma (PDA). Overexpression of MYC and deregulation of retinoblastoma (RB) further promote cell proliferation and make identifying a means to therapeutically alter cell-cycle control pathways in PDA a significant challenge. We previously showed that the basic helix-loop-helix transcription factor E47 induced stable growth arrest in PDA cells in vitro and in vivo. Here, we identified molecular mechanisms that underlie E47-induced growth arrest in low-passage, patient-derived primary and established PDA cell lines. METHODS: RNA sequencing was used to profile E47-dependent transcriptomes in 5 PDA cell lines. Gene Ontology analysis identified cell-cycle control as the most altered pathway. Small interfering RNA/short hairpin RNA knockdown, small-molecule inhibitors, and viral expression were used to examine the function of E47-dependent genes in cell-cycle arrest. Cell morphology, expression of molecular markers, and senescence-associated ß-galactosidase activity assays identified cellular senescence. RESULTS: E47 uniformly inhibited PDA cell-cycle progression by decreasing expression of MYC, increasing the level of CDKN1B/p27KIP1, and restoring RB tumor-suppressor function. The molecular mechanisms by which E47 elicited these changes included altering both RNA transcript levels and protein stability of MYC and CDKN1B/p27KIP1. At the cellular level, E47 elicited a senescence-like phenotype characterized by increased senescence-associated ß-galactosidase activity and altered expression of senescence markers. CONCLUSIONS: E47 governs a highly conserved network of cell-cycle control genes, including MYC, CDKN1B/p27KIP1, and RB, which can induce a senescence-like program in PDA cells that lack CDKN2A/p16INK4A and wild-type p53. RNA sequencing data are available at the National Center for Biotechnology Information GEO at https://www.ncbi.nlm.nih.gov/geo/; accession number: GSE100327.

3.
Proc Natl Acad Sci U S A ; 113(47): 13408-13413, 2016 11 22.
Article in English | MEDLINE | ID: mdl-27810956

ABSTRACT

As a key component of the vertebrate neuroendocrine system, the pituitary gland relies on the progressive and coordinated development of distinct hormone-producing cell types and an invading vascular network. The molecular mechanisms that drive formation of the pituitary vasculature, which is necessary for regulated synthesis and secretion of hormones that maintain homeostasis, metabolism, and endocrine function, remain poorly understood. Here, we report that expression of integrin ß1 in embryonic pituitary epithelial cells is required for angiogenesis in the developing mouse pituitary gland. Deletion of pituitary epithelial integrin ß1 before the onset of angiogenesis resulted in failure of invading endothelial cells to recruit pericytes efficiently, whereas deletion later in embryogenesis led to decreased vascular density and lumen formation. In both cases, lack of epithelial integrin ß1 was associated with a complete absence of vasculature in the pituitary gland at birth. Within pituitary epithelial cells, integrin ß1 directs a large transcriptional program that includes components of the extracellular matrix and associated signaling factors that are linked to the observed non-cell-autonomous effects on angiogenesis. We conclude that epithelial integrin ß1 functions as a critical and canonical regulator of developmental angiogenesis in the pituitary gland, thus providing insight into the long-standing systems biology conundrum of how vascular invasion is coordinated with tissue development.


Subject(s)
Embryonic Development , Epithelial Cells/metabolism , Integrin beta1/metabolism , Neovascularization, Physiologic , Pituitary Gland/cytology , Pituitary Gland/embryology , Animals , Animals, Newborn , Cell Count , Cell Differentiation , Embryonic Development/genetics , Endothelial Cells/cytology , Endothelial Cells/metabolism , Extracellular Matrix/metabolism , Gene Deletion , Gene Expression Regulation, Developmental , Gene Targeting , Integrases/metabolism , Mice , Neovascularization, Physiologic/genetics , Paired Box Transcription Factors/metabolism , Pericytes/cytology , Pericytes/metabolism , Phenotype , Pituitary Gland/metabolism , Sequence Analysis, RNA , Time Factors , Vascular Endothelial Growth Factor C/metabolism
4.
Science ; 295(5563): 2231-5, 2002 Mar 22.
Article in English | MEDLINE | ID: mdl-11910101

ABSTRACT

During mammalian pituitary gland development, distinct cell types emerge from a common primordium. Appearance of specific cell types occurs in response to opposing signaling gradients that emanate from distinct organizing centers. These signals induce expression of interacting transcriptional regulators, including DNA binding-dependent activators and DNA binding-independent transrepressors, in temporally and spatially overlapping patterns. Together they synergistically regulate precursor proliferation and induction of distinct cell types. Terminal cell type differentiation requires selective gene activation strategies and long-term active repression, mediated by cell type-specific and promoter-specific recruitment of coregulatory complexes. These mechanisms imply the potential for flexibility in the ultimate identity of differentiated cell types.


Subject(s)
Cell Lineage , Gene Expression Regulation, Developmental , Pituitary Gland/cytology , Pituitary Gland/embryology , Transcription Factors/metabolism , Animals , Cell Differentiation , Homeodomain Proteins/metabolism , Mammals/embryology , Pituitary Gland/metabolism , Pro-Opiomelanocortin/metabolism , Signal Transduction , Transcriptional Activation
SELECTION OF CITATIONS
SEARCH DETAIL
...