ABSTRACT
Most adult patients with acute non-lymphoblastic leukaemia (ANLL) relapse with drug resistance. Overexpression of a plasma membrane protein, P-glycoprotein, correlates with multidrug resistance in human and animal cell lines. We have detected a multidrug resistance phenotype in two patients with drug resistant ANLL by an immunocytochemical assay using a monoclonal antibody to P-glycoprotein. Sequential analysis of peripheral blood samples from both patients showed a progressive increase in both the intensity of staining and the proportion of leukaemic cells that bound antibody as the disease progressed. The assay is simple, and may have prognostic and therapeutic implications.
Subject(s)
Glycoproteins/blood , Leukemia/blood , ATP Binding Cassette Transporter, Subfamily B, Member 1 , Acute Disease , Antibodies, Monoclonal , Cell Line , Drug Resistance , Female , Glycoproteins/immunology , Humans , Leukemia/drug therapy , Male , Middle Aged , PhenotypeSubject(s)
Hyaline Membrane Disease/diagnosis , Infant, Newborn, Diseases/diagnosis , Pneumonia/diagnosis , Diagnosis, Differential , Female , Humans , Hyaline Membrane Disease/blood , Hyaline Membrane Disease/microbiology , Infant, Newborn , Infant, Newborn, Diseases/blood , Infant, Newborn, Diseases/microbiology , Leukocyte Count , Male , Pneumonia/blood , Pneumonia/microbiologyABSTRACT
Leukaphereses were performed on the intermittent flow cell separator (Haemonentics Model 30) using citrated dextran 150 as a macromolecular agent to improve separation of granulocytes. A mean of 1.77 X 10(10) granulocytes and 7.73 X 10(11) platelets were obtained without steroid stimulation and 2.65 X 10(10) granulocytes and 8.91 X 10(11) platelets were obtained with steroid stimulation of donors. There were no significant adverse reactions to dextran.
