1.
Org Lett
; 4(14): 2305-8, 2002 Jul 11.
Article
in English
| MEDLINE
| ID: mdl-12098233
ABSTRACT
[structure: see text] A fluorescent deoxyuridine analogue is sensitive to the polarity of its environment and exhibits a distinct emission profile in single- vs double-stranded oligonucleotides. Emission-monitored denaturation curves of internally modified dU(phen) duplexes are characteristic of the base opposite dU(phen) and distinguish between perfect and mismatched complementary oligonucleotides.