ABSTRACT
Drylands typically have strong seasonal variation in rainfall and primary productivity. This study examines the effects of seasonal change in grass-derived resource availability on the base of the food chain of a mammalian predator. Seasonal changes in live grass cover were measured in two vegetation types at the Sevilleta National Wildlife Refuge in central New Mexico, USA. Non-invasive genetic sampling of scat was used to identify individuals in the local coyote (Canis latrans) population. Stable carbon and nitrogen isotope analysis of hair removed from scats of 45 different coyotes was used to assess seasonal variation in the diet of mammalian coyote prey that came from C4 grasses. Live grass cover increased from the spring to the summer and fall; contribution of C4 grasses to the diet of mammalian coyote prey increased from the summer to the fall and was higher in grassland areas. There were significant differences in the seasonal patterns in the prey diet between grassland and shrubland areas.