Experimental Research of Fibrous Materials for Two-Stage Filtration of the Intake Air of Internal Combustion Engines.

Pollutant properties in intake air to internal combustion engines were analyzed. Mineral dust particles' influence on accelerated engine components' wear was discussed. Dust concentration values in the air under various operating conditions in trucks and special vehicles were presented. The idea and necessity for using two-stage filters, operating in a "multi-cyclone-porous partition" system for vehicles operated in dusty air conditions, are presented. Information from the literature information has been presented, showing that impurities in small grain sizes reduce fiber bed absorbency. It has been shown that such a phenomenon occurs during filter material operation, located directly behind the inertial filter (multi-cyclone), which off-road vehicles are equipped with. It results in a greater pressure drop intensity increase and a shorter proper filter operation period. It has been shown that filter material selection for the motor vehicle air filter requires knowledge of the mass of stopped dust per filtration unit area (dust absorption coefficient km) determined for a given permissible resistance value Δpfdop. It has been shown that there is no information on absorption coefficient values for filter materials operating in a two-stage "multi-cyclone-porous partition" separation system. Original methodology and conditions for determining dust absorption coefficient (km) of a separation partition, operating under the conditions of two-stage filtration, were presented. The following characteristics were tested: separation efficiency, filtration performance, and pressure drop characteristics of three different filtration partitions. These were A (cellulose), B (cellulose and polyester), and C (cellulose, polyester, and nanofibers layer), working individually and in a two-stage system-behind the cyclone. Granulometric dust composition dosed into the cyclone and cyclone downstream was determined. During tests, conditions corresponding to air filter's actual operating conditions, including separation speed and dust concentration in the air, were maintained. For the pressure drop values, the dust absorption coefficient (km) values of three different filtration partitions (A, B, and C), working individually and in a two-stage system-behind the cyclone-were determined experimentally.

Experimental Study of Filtration Materials Used in the Car Air Intake.

Traditional cellulose filter media used for air filtration in vehicle engines are characterized by 99.9% filtration efficiency and accuracy above 2-5 µm. The highest engine component wear is caused by dust grains above 1 µm. Filter media with nanofiber additions provide greater filtration efficiency of dust grains below 5 µm. Filter material selection for vehicle engine air filter is a problem because their manufacturers mainly provide only the structure parameters: pore size, air permeability, and thickness. There is no information about material filtration properties using polydisperse test dust. The manuscript presents methodology and experimental test results of five samples A, B, C, D and E, filter materials differing in their chemical composition and structure parameters. In the first stage, efficiency characteristics φw, filtration accuracy dzmax and the flow resistance Δpw depending on the dust absorption coefficient km of three filter cartridges of each material, A, B, C, D and E, were determined. Then, from each material characteristics of one piece was selected in order to compare their initial and initial period efficiencies as well as changes in the flow resistance depending on the dust absorption coefficient km. Obtained results showed that the filter materials differ significantly in efficiency and accuracy values in the initial filtration period. Initial period duration is also different, i.e., filtration efficiency increasing time to a certain value, which for materials with a nanofiber layer is much shorter, which minimizes engine component wear. For materials with nanofibers, flow resistance increase intensity is greater, which results from surface filtration. Filtration efficiency of each filter material sample A, B, C, D and E was assessed with the filtration quality coefficient including the efficiency and flow resistance. In the available literature, the problem of increasing filtration efficiency in the initial period is known, but there are no results for specific filter materials. Research shows that filter material characteristics are closely related. Each increase in efficiency and accuracy of intake air filtration reduces engine components wear, but it is related to flow resistance increase in the engine intake system, which reduces its power, and increases need for more frequent filter servicing.

Impaired DNA demethylation of C/EBP sites causes premature aging.

Changes in DNA methylation are among the best-documented epigenetic alterations accompanying organismal aging. However, whether and how altered DNA methylation is causally involved in aging have remained elusive. GADD45α (growth arrest and DNA damage protein 45A) and ING1 (inhibitor of growth family member 1) are adapter proteins for site-specific demethylation by TET (ten-eleven translocation) methylcytosine dioxygenases. Here we show that Gadd45a/Ing1 double-knockout mice display segmental progeria and phenocopy impaired energy homeostasis and lipodystrophy characteristic of Cebp (CCAAT/enhancer-binding protein) mutants. Correspondingly, GADD45α occupies C/EBPß/δ-dependent superenhancers and, cooperatively with ING1, promotes local DNA demethylation via long-range chromatin loops to permit C/EBPß recruitment. The results indicate that enhancer methylation can affect aging and imply that C/EBP proteins play an unexpected role in this process. Our study suggests a causal nexus between DNA demethylation, metabolism, and organismal aging.

Phosphorylation of the yeast γ-tubulin Tub4 regulates microtubule function.

The yeast γ-tubulin Tub4 is assembled with Spc97 and Spc98 into the small Tub4 complex. The Tub4 complex binds via the receptor proteins Spc72 and Spc110 to the spindle pole body (SPB), the functional equivalent of the mammalian centrosome, where the Tub4 complex organizes cytoplasmic and nuclear microtubules. Little is known about the regulation of the Tub4 complex. Here, we isolated the Tub4 complex with the bound receptors from yeast cells. Analysis of the purified Tub4 complex by mass spectrometry identified more than 50 phosphorylation sites in Spc72, Spc97, Spc98, Spc110 and Tub4. To examine the functional relevance of the phosphorylation sites, phospho-mimicking and non-phosphorylatable mutations in Tub4, Spc97 and Spc98 were analyzed. Three phosphorylation sites in Tub4 were found to be critical for Tub4 stability and microtubule organization. One of the sites is highly conserved in γ-tubulins from yeast to human.