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1.
J Agric Food Chem ; 55(3): 693-9, 2007 Feb 07.
Article in English | MEDLINE | ID: mdl-17263462

ABSTRACT

Several properties of chitosan films associated or not with hydroxypropylmethylcellulose polymer (HPMC) and HPMC films incorporating or not nisin and/or milk fat were studied. Nisin addition at a level of 250 microg mL-1 and likewise chitosan at 1% (w/v) concentration were efficient for total inhibiting Aspergillus niger and Kocuria rhizophila food deterioration microorganisms. HPMC and chitosan films were transparent, whereas nisin and/or fat incorporation induced a 2-fold lightness parameter increase and, consequently, involved more white films. Measurements of tensile strength, as well as ultimate elongation, showed that chitosan and HPMC initial films were elastic and flexible. High thermal treatments and additive incorporation induced less elastic and more plastic films. Water vapor transmission as far as total water desorption rates suggested that chitosan films were slightly sensitive to water. Water transfer was decreased by <60% as compared with other biopolymer films. Regarding its hydrophobic property, the capacity of fat to improve film water barrier was very limited.


Subject(s)
Anti-Infective Agents/pharmacology , Chitosan/chemistry , Food Packaging/instrumentation , Methylcellulose/analogs & derivatives , Water/chemistry , Animals , Aspergillus niger/drug effects , Chemical Phenomena , Chemistry, Physical , Chitosan/pharmacology , Fats/chemistry , Hypromellose Derivatives , Methylcellulose/chemistry , Methylcellulose/pharmacology , Micrococcaceae/drug effects , Milk/chemistry , Nisin/chemistry
2.
J Agric Food Chem ; 51(22): 6468-74, 2003 Oct 22.
Article in English | MEDLINE | ID: mdl-14558764

ABSTRACT

Cross-linked hydroxypropylmethylcellulose (HPMC) cast films with citric acid as polycarboxylic cross-linker were elaborated to study the effect of cross-linking level on various properties. Increased amounts of cross-linking agent were not connected to statistically different tensile strength and Young's modulus. Whatever the cross-linking level of the film was, the ultimate elongation parameter decreased by approximately 60% compared to the HMPC control film. Moisture sorption isotherms and water contact angle meter showed that the effect of cross-linking degree tends to reduce the hygroscopic and hydrophilic characteristics of films. In addition, to control bacteria growth on food surfaces, the antimicrobial activity of both 98% cross-linked HPMC-nisin and control HPMC-nisin films was tested on Micrococcus luteus. Despite the incorporation of a significant content of nisin, cross-linked HPMC-nisin films were completely inactive on the microbial strain compared to the HPMC-nisin control films. Cross-linking conditions likely either denatured the nisin or irreversibly bound nisin to the cross-linked HPMC. However, nisin adsorbed into films made from previously cross-linked HPMC maintained its activity.


Subject(s)
Food Packaging , Methylcellulose/chemistry , Nisin/analysis , Anti-Bacterial Agents , Chemical Phenomena , Chemistry, Physical , Citric Acid/chemistry , Cross-Linking Reagents , Food Microbiology , Food Packaging/instrumentation , Hypromellose Derivatives , Methylcellulose/analogs & derivatives , Nisin/chemistry , Tensile Strength , Water/chemistry
3.
J Agric Food Chem ; 50(15): 4290-4, 2002 Jul 17.
Article in English | MEDLINE | ID: mdl-12105960

ABSTRACT

To develop biodegradable packaging that both acts as a moisture barrier and as antimicrobial activity, nisin and stearic acid were incorporated into a hydroxy propyl methyl cellulose (HPMC) based film. Fifteen percent (w/w HPMC) of stearic acid improved film moisture barrier. However, film mechanical resistance and film antimicrobial activity on Listeria monocytogenes and Staphylococcus aureus pathogenic strains were both reduced. This lower film inhibitory activity was due to interactions between nisin and stearic acid. The molecular interaction was modeled, and an equation was developed to calculate the nisin concentration needed to be incorporated into the film matrix to obtain a desired residual antimicrobial activity. Because the molecular interactions were pH dependent, the impact of the pH of the film-forming solution on film inhibitory activity was investigated. Adjusting the pH to 3 totally avoided stearic acid and nisin interaction, inducing a high film inhibitory activity.


Subject(s)
Cellulose , Fatty Acids , Food Packaging , Methylcellulose/analogs & derivatives , Anti-Bacterial Agents/pharmacology , Drug Interactions , Hydrogen-Ion Concentration , Hypromellose Derivatives , Listeria monocytogenes/drug effects , Nisin/pharmacology , Staphylococcus aureus/drug effects , Stearic Acids/pharmacology
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