ABSTRACT
The novel HLA-A*02:1140 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , HLA-A2 Antigen , Humans , Brazil , HLA-A2 Antigen/genetics , HLA-A2 Antigen/immunology , Histocompatibility Testing , Exons , Tissue Donors , Base Sequence , Sequence Analysis, DNA/methods , Sequence AlignmentABSTRACT
The novel HLA-C*15:274 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , Exons , HLA-C Antigens , Histocompatibility Testing , Humans , HLA-C Antigens/genetics , Histocompatibility Testing/methods , Sequence Analysis, DNA/methods , Tissue Donors , Brazil , High-Throughput Nucleotide Sequencing/methods , Base SequenceABSTRACT
The novel HLA-C*08:275 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , Exons , HLA-C Antigens , Humans , HLA-C Antigens/genetics , Brazil , Histocompatibility Testing , Tissue Donors , Base Sequence , Sequence Analysis, DNA/methods , Sequence Alignment , CodonABSTRACT
The novel HLA-B*35:593 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , Exons , Humans , Histocompatibility Testing/methods , Sequence Analysis, DNA/methods , Tissue Donors , Brazil , HLA-B35 Antigen/genetics , HLA-B Antigens/genetics , High-Throughput Nucleotide Sequencing/methods , Base SequenceABSTRACT
The novel HLA-A*30:218 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Alleles , Exons , HLA-A Antigens , Histocompatibility Testing , Sequence Analysis, DNA , Humans , HLA-A Antigens/genetics , Sequence Analysis, DNA/methods , Histocompatibility Testing/methods , Tissue Donors , Base Sequence , Brazil , Sequence AlignmentABSTRACT
To control immune responses, regulatory CD4+CD25+Foxp3+ T cells (Treg) maintain their wide and diverse repertoire through continuous arrival of recent thymic emigrants (RTE). However, during puberty, the activity of RTE starts to decline as a natural process of thymic involution, introducing consequences, not completely described, to the repertoire. Type 1 diabetes (T1D) patients show quantitative and qualitative impairments on the Treg cells. Our aim was to evaluate peripheral Treg and RTE cell frequencies, in T1D patients from two distinct age groups (young and adults) and verify if HLA phenotypes are concomitant associated. To this, blood samples from Brazilian twenty established T1D patients (12 young and 8 adults) and twenty-one healthy controls (11 young and 10 adults) were analyzed, by flow cytometry, to verify the percentages of CD4, Treg (CD4+CD25+Foxp3+) and the subsets of CD45RA+ (naive) and CD31+(RTE) within then. Furthermore, the HLA typing was also set. We observed that the young established T1D patients feature decreased frequencies in total Treg cells and naive RTE within Treg cells. Significant prevalence of HLA alleles, associated with risk, in T1D patients, was also identified. Performing a multivariate analysis, we confirmed that the cellular changes described offers significant variables that distinct T1D patients from the controls. Our data collectively highlight relevant aspects about homeostasis imbalances in the Treg cells of T1D patients, especially in young, and disease prognosis; that might contribute for future therapeutic strategies involving Treg cells manipulation.
Subject(s)
Diabetes Mellitus, Type 1 , Forkhead Transcription Factors , Interleukin-2 Receptor alpha Subunit , T-Lymphocytes, Regulatory , Thymus Gland , Humans , Diabetes Mellitus, Type 1/immunology , T-Lymphocytes, Regulatory/immunology , T-Lymphocytes, Regulatory/metabolism , Adult , Brazil , Male , Female , Forkhead Transcription Factors/metabolism , Thymus Gland/immunology , Interleukin-2 Receptor alpha Subunit/metabolism , Young Adult , Adolescent , Immunophenotyping , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , ChildABSTRACT
The novel HLA-DQB1*03:491 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
High-Throughput Nucleotide Sequencing , Polymorphism, Single Nucleotide , Humans , Alleles , Brazil , Bone Marrow Transplantation , HLA-DQ beta-Chains/geneticsABSTRACT
The novel HLA-C*04:01:145 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
HLA-C Antigens , High-Throughput Nucleotide Sequencing , Alleles , Bone Marrow Transplantation , Brazil , HLA-C Antigens/genetics , Humans , Polymorphism, Single NucleotideABSTRACT
The novel HLA-B*41:01:08 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
High-Throughput Nucleotide Sequencing , Polymorphism, Single Nucleotide , Alleles , Bone Marrow Transplantation , Brazil , HLA-B Antigens/genetics , HumansABSTRACT
The novel HLA-DRB1*03:196 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Bone Marrow Transplantation , Polymorphism, Single Nucleotide , Alleles , Brazil , HLA-DRB1 Chains/genetics , High-Throughput Nucleotide Sequencing , HumansABSTRACT
AIMS: This study aimed to identify the symptoms associated with early stage SARS-CoV-2 (COVID-19) infections in healthcare professionals (HCPs) using both clinical and laboratory data. METHODS: A total of 1297 patients, admitted between 18 March and 8 April 2020, were stratified according to their risk of developing COVID-19 using their responses to a questionnaire designed to evaluate symptoms and risk conditions. RESULTS: Anosmia/hyposmia (p<0.0001), fever (p<0.0001), body pain (p<0.0001) and chills (p=0.001) were all independent predictors for COVID-19, with a 72% estimated probability for detecting COVID-19 in nasopharyngeal swab samples. Leucopenia, relative monocytosis, decreased eosinophil values, C reactive protein (CRP) and platelets were also shown to be significant independent predictors for COVID-19. CONCLUSIONS: The significant clinical features for COVID-19 were identified as anosmia, fever, chills and body pain. Elevated CRP, leucocytes under 5400×109/L and relative monocytosis (>9%) were common among patients with a confirmed COVID-19 diagnosis. These variables may help, in the absence of reverse transcriptase PCR tests, to identify possible COVID-19 infections during pandemic outbreaks. SUMMARY: From 19 March to 8 April 2020, 1297 patients attended the Polyclinic Piquet Carneiro for COVID-19 detection. HCP data were analysed, and significant clinical features were anosmia, fever, chills and body pain. Elevated CRP, leucopenia and monocytosis were common in COVID-19.
Subject(s)
COVID-19/pathology , SARS-CoV-2/isolation & purification , Adult , Brazil/epidemiology , COVID-19/diagnosis , COVID-19/epidemiology , Diagnostic Screening Programs , Female , Health Personnel , Humans , Male , Middle Aged , Outpatients , Pandemics , SARS-CoV-2/geneticsABSTRACT
The novel HLA-A*02:01:202 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Bone Marrow , High-Throughput Nucleotide Sequencing , Alleles , Brazil , HLA-A Antigens/genetics , HumansABSTRACT
The novel HLA-A*29:158 allele, first described in potential bone marrow donors from Brazil.
Subject(s)
Bone Marrow , High-Throughput Nucleotide Sequencing , Alleles , Brazil , HLA-A Antigens/genetics , HumansABSTRACT
The novel HLA-A*33:221 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Bone Marrow , High-Throughput Nucleotide Sequencing , Alleles , Brazil , HLA-A Antigens/genetics , HumansABSTRACT
The sharp increase of COVID-19 cases in late 2020 has made Brazil the new epicenter of the ongoing SARS-CoV-2 pandemic. The novel viral lineages P.1 (Variant of Concern Gamma) and P.2, respectively identified in the Brazilian states of Amazonas and Rio de Janeiro, have been associated with potentially higher transmission rates and antibody neutralization escape. In this study, we performed the whole-genome sequencing of 185 samples isolated from three out of the five Brazilian regions, including Amazonas (North region), Rio Grande do Norte, Paraíba and Bahia (Northeast region), and Rio de Janeiro (Southeast region) in order to monitor the spread of SARS-CoV-2 lineages in Brazil in the first months of 2021. Here, we showed a widespread dispersal of P.1 and P.2 across Brazilian regions and, except for Amazonas, P.2 was the predominant lineage identified in the sampled states. We estimated the origin of P.2 lineage to have happened in February, 2020 and identified that it has differentiated into new clades. Interstate transmission of P.2 was detected since March, but reached its peak in December, 2020 and January, 2021. Transmission of P.1 was also high in December and its origin was inferred to have happened in August 2020. We also confirmed the presence of lineage P.7, recently described in the southernmost region of Brazil, to have spread across the Northeastern states. P.1, P.2 and P.7 are descended from the ancient B.1.1.28 strain, which co-dominated the first phase of the pandemic in Brazil with the B.1.1.33 strain. We also identified the occurrence of a new lineage descending from B.1.1.33 that convergently carries the E484K mutation, N.9. Indeed, the recurrent report of many novel SARS-CoV-2 genetic variants in Brazil could be due to the absence of effective control measures resulting in high SARS-CoV2 transmission rates. Altogether, our findings provided a landscape of the critical state of SARS-CoV-2 across Brazil and confirm the need to sustain continuous sequencing of the SARS-CoV-2 isolates worldwide in order to identify novel variants of interest and monitor for vaccine effectiveness.
Subject(s)
COVID-19/epidemiology , COVID-19/virology , Genome, Viral , Genomics/methods , SARS-CoV-2 , Brazil/epidemiology , COVID-19/transmission , Genetic Variation , High-Throughput Nucleotide Sequencing , Humans , Phylogeny , SARS-CoV-2/classification , SARS-CoV-2/geneticsABSTRACT
The novel HLA-DQA1*03:03:05 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
High-Throughput Nucleotide Sequencing , Polymorphism, Single Nucleotide , Alleles , Brazil , HLA-DQ alpha-Chains/genetics , HumansABSTRACT
The novel HLA-A*68:272 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
Bone Marrow Transplantation , Bone Marrow , Alleles , Brazil , HLA-A Antigens/genetics , High-Throughput Nucleotide Sequencing , HumansABSTRACT
The novel HLA-DPA1*04:03 allele, first described in a potential bone marrow donor from Brazil.
Subject(s)
HLA-DP alpha-Chains , High-Throughput Nucleotide Sequencing , Alleles , Brazil , HLA-DP alpha-Chains/genetics , HumansABSTRACT
INTRODUCTION: Rio de Janeiro has hardly experienced coronavirus disease. METHODS: Here, 87,442 reverse transcription polymerase chain reaction (RT-PCR) test results for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were reported among Rio de Janeiro residents (March to September 2020). RESULTS: Overall, RT-PCR positivity of 44.6% decreased over time towards 20%. Positivity was greater among males (OR=1.22; 95%CI:1.19-1.26); Black (OR=1.10; 95%CI:1.02-1.19), Brown (OR=1.16; 95%CI:1.10-1.22), and indigenous people (OR=2.11; 95%CI:0.88-5.03) compared to Whites and increased with age; with epidemic spread from the capital to inland regions. CONCLUSIONS: SARS-CoV-2 keeps spreading in Rio de Janeiro, and reopening of activities may fuel the epidemic.
Subject(s)
COVID-19 , SARS-CoV-2 , Brazil/epidemiology , Humans , Incidence , Male , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
Abstract INTRODUCTION Rio de Janeiro has hardly experienced coronavirus disease. METHODS Here, 87,442 reverse transcription polymerase chain reaction (RT-PCR) test results for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were reported among Rio de Janeiro residents (March to September 2020). RESULTS Overall, RT-PCR positivity of 44.6% decreased over time towards 20%. Positivity was greater among males (OR=1.22; 95%CI:1.19-1.26); Black (OR=1.10; 95%CI:1.02-1.19), Brown (OR=1.16; 95%CI:1.10-1.22), and indigenous people (OR=2.11; 95%CI:0.88-5.03) compared to Whites and increased with age; with epidemic spread from the capital to inland regions. CONCLUSIONS SARS-CoV-2 keeps spreading in Rio de Janeiro, and reopening of activities may fuel the epidemic.
