ABSTRACT
Trichomonas vaginalis infections are usually asymptomatic or can result in nonspecific clinical symptoms, which makes laboratory-based detection of this protozoan parasite essential for diagnosis and treatment. We report the development of a battery of highly sensitive and specific PCR assays for detection of T. vaginalis in urine, a noninvasive specimen, and development of a protocol for differentiating among Trichomonas species that commonly infect humans.
Subject(s)
Clinical Laboratory Techniques/methods , Molecular Diagnostic Techniques/methods , Parasitology/methods , Polymerase Chain Reaction/methods , Trichomonas Infections/diagnosis , Trichomonas vaginalis/isolation & purification , Urine/parasitology , Adolescent , Child , Child, Preschool , Female , Humans , Infant , Sensitivity and Specificity , Trichomonas vaginalis/classification , Trichomonas vaginalis/geneticsABSTRACT
To determine seropositivity for Enterocytozoon bieneusi in the Czech Republic, we tested 115 serum samples from various groups. We found that 20% from HIV-positive persons, 33% from persons with occupational exposure to animals, and 10% from healthy persons were positive by indirect immunofluorescence assay. Proteins of 32 kDa were detected in serum samples from seropositive persons.
