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Nucleic Acids Res ; 28(10): 2128-34, 2000 May 15.
Article in English | MEDLINE | ID: mdl-10773082

ABSTRACT

The synthesis and triplex stabilizing properties of oligodeoxyribonucleotides functionalized at the 5'- and/or 3'-termini with a naphthalene diimide-based (NDI) intercalator is described. The NDI intercalator was prepared in a single step from the corresponding dianhydride and was attached to the 5'-terminus of an oligodeoxyribonucleotide following a reverse coupling procedure. The DMT protecting group was removed and the sequence phosphitylated to generate the phosphoramidite derivative on the 5'-terminus of the support-bound oligodeoxyribonucleotide. The NDI intercalator with a free hydroxyl was then added in the presence of tetrazole. Attachment of the NDI to the 3'-terminus relied upon a tethered amino group that could be functionalized first with the naphthalene dianhydride, which was subsequently converted to the diimide. Using both procedures, an oligonucleo-tide conjugate was prepared having the NDI intercalator at both the 5'- and 3'-termini. Thermal denaturation studies were used to determine the remarkable gain in stability for triplexes formed when the NDI-conjugated oligonucleotide was present as the third strand in the complex.


Subject(s)
DNA/chemistry , Imides/chemical synthesis , Intercalating Agents/chemical synthesis , Nucleic Acid Conformation , Oligodeoxyribonucleotides/chemistry , Base Sequence , Chromatography, High Pressure Liquid , Imides/chemistry , Indicators and Reagents , Intercalating Agents/chemistry , Naphthalenes , Nuclear Magnetic Resonance, Biomolecular , Phenanthrolines , Thermodynamics
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