ABSTRACT
A useful synthesis of (R)-[N-methyl-3 H]quinuclidinyl benzilate methiodide is described with the product characterized by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), tritium nuclear magnetic resonance (NMR), and mass spectrometry (MS). Several methods are provided to purify the radioligand, and its storage and stability are also discussed.
Subject(s)
Chemistry Techniques, Synthetic/methods , Cholinergic Antagonists/chemistry , Cholinergic Antagonists/chemical synthesis , Quinuclidinyl Benzilate/chemistry , Quinuclidinyl Benzilate/chemical synthesis , Tritium/chemistry , RadiochemistryABSTRACT
Methods are presented to tritiate the side chain of dopamine.
Subject(s)
Dopamine/chemistry , Isotope Labeling/methods , Tritium , Magnetic Resonance SpectroscopyABSTRACT
Beta-funaltrexamine is a unique irreversible antagonist for the mu-opiate receptor and would be useful as a tritiated radioligand. Starting from high specific activity [15, 16-³H] naltrexone, [15, 16-³H] beta-funaltrexamine was synthesized and characterized by means of a two-step reductive amination-acylation process.
Subject(s)
Isotope Labeling/methods , Naltrexone/analogs & derivatives , Radiopharmaceuticals/chemical synthesis , Naltrexone/chemical synthesis , Tritium/chemistryABSTRACT
Methods are presented to synthesize and characterize [propyl-(3)H] trefentanil 3 and [propenyl-(3)H] naloxonazine 6.
Subject(s)
Isotope Labeling/methods , Magnetic Resonance Spectroscopy/methods , Naloxone/analogs & derivatives , Piperidines/chemistry , Receptors, Opioid, mu/analysis , Receptors, Opioid, mu/chemistry , Tetrazoles/chemistry , Tritium/chemistry , Naloxone/analysis , Naloxone/chemistry , Piperidines/analysis , Radiopharmaceuticals/analysis , Radiopharmaceuticals/chemical synthesis , Tetrazoles/analysis , Tritium/analysisABSTRACT
Methods are presented for tritiating the D2 specific dopaminergic antagonist domperidone and D3 specific dopaminergic agonist (+/-)-7-hydroxy DPAT. Techniques to characterize the products of the tritiation are also given.
