ABSTRACT
BACKGROUND: Data regarding yeast microbiota in goat milk is scarce. AIMS: To isolate and identify species of the genus Candida in milk samples from clinically healthy goats, and evaluate their enzymatic activity and biofilm formation. METHODS: 1092 milk samples from clinically healthy goats were collected and processed. The yeast isolates were identified by phenotypic, methods and their enzymatic activity (phospholipase, hemolysin and protease) and biofilm formation evaluated. RESULTS: We obtained 221 Candida isolates belonging to six species: Candida kefyr (35.7%), Candida guilliermondii (33%), Candida famata (23.5%), Candida glabrata (5.9%), Candida albicans (1.35%) and Candida parapsilosissensu lato (0.45%). Protease activity was detected in all Candida species while hemolysin activity was only present in C. kefyr, C. guilliermondii, C. famata and C. albicans. Only C. albicans showed phospholipase activity. With the exception of C. parapsilosis sensu lato, all Candida species formed biofilm, with 60.19% of the isolates being poor producers, 9.93% moderate producers, and 1.35% strong producers. CONCLUSIONS: The milk of clinically healthy goats contains several species of the genus Candida that could play a role as opportunistic pathogens in mastitis.
Subject(s)
Candida , Milk , Animals , Biofilms , Candida albicans , Female , GoatsABSTRACT
Antecedentes. Las mastitis subclínicas y clínicas en bovinos por hongos se han incrementado principalmente por levaduras del género Candida. Objetivo. Conocer las levaduras presentes en la leche de glándulas mamarias de bovinos clínicamente sanos, y de aquellos con mastitis subclínica y clínica. Método. Se evaluó la presencia de levaduras en 1.095 muestras de leche de 342 glándulas mamarias sanas, 383 con mastitis subclínica y 370 con mastitis clínica, de los estados de Querétaro, Hidalgo, Puebla y la ciudad de México, Distrito Federal, que forman parte del Altiplano Mexicano. El estado sanitario de las glándulas mamarias se determinó por examen clínico y la prueba de California. La identificación de levaduras fue realizada por métodos morfológicos y bioquímicos. Resultados. Se identificaron 20 especies diferentes del género Candida a partir de 282 (25,75%) de las muestras de leche. Las especies encontradas con mayor frecuencia en los bovinos sanos y con mastitis clínica fueron Candida glabrata y Candida krusei. El grupo de las muestras con mastitis subclínica mostró una diversidad de especies de Candida, incluidas Candida zeylanoides, Candida norvegica, Candida viswanathii, Candida guilliermondii y Candida tropicalis. Candida albicans fue aislada solo en 11 (3,9%) de las muestras de mastitis clínica (6) y subclínica (5). Conclusiones. Estos resultados sugieren el posible papel de otras especies de Candida diferentes a C. albicans como causantes de mastitis micótica(AU)
Background. The mastitis subclinical and clinical in cows caused by fungi has been increased specially by yeast of the genus Candida. Objective. To identify what yeasts were present in milk samples obtained from mammary glands of healthy cows, and others suffering subclinical or clinical mastitis. Methods. From a total of 1,095 milk samples 342 were from mammary glands of healthy dairy cows, 383 with subclinical mastitis, and 370 with clinical mastitis, were taken, in the states of Querétaro, Hidalgo, Puebla and Mexico City (Distrito Federal) in the so called Mexican High Plateu. The clinical status of the mammary glands was determined by clinical examination and the California Mastitis Test. Yeasts identification was carried out by morphology and biochemical methods. Results. Twenty different species of Candida were identified out of 282 (25.75%) milk samples. The most frequently identified species in the healthy cows and cows with clinical mastitis groups were Candida glabrata and Candida krusei. On the other hand, samples from the subclinical mastitis group showed a diversity of Candida species, including Candida zeylanoides, Candida norvegica, Candida viswanathii, Candida guilliermondii, and Candida tropicalis. Candida albicans was isolated only in 11 (3.9%) samples from the clinical and subclinical mastitis groups. Conclusions. These results suggest the possible role that Candida species other than C. albicans may play in mycotic mastitis in cows(AU)
