ABSTRACT
The genus Psychrobacter contains environmental, psychrophilic and halotolerant gram-negative bacteria considered rare opportunistic pathogens in humans. Metagenomics was performed on the cerebrospinal fluid (CSF) of a pediatric patient with meningitis. Nucleic acids were extracted, randomly amplified, and sequenced with the 454 GS FLX Titanium next-generation sequencing (NGS) system. Sequencing reads were assembled, and potential virulence genes were predicted. Phylogenomic and phylogenetic studies were performed. Psychrobacter sp. 310 was identified, and several virulence genes characteristic of pathogenic bacteria were found. The phylogenomic study and 16S rRNA gene phylogenetic analysis showed that the closest relative of Psychrobacter sp. 310 was Psychrobacter sanguinis. To our knowledge, this is the first report of a meningitis case associated with Psychrobacter sp. identified by NGS metagenomics in CSF from a pediatric patient. The metagenomic strategy based on NGS was a powerful tool to identify a rare unknown pathogen in a clinical case.
Subject(s)
Cerebrospinal Fluid/microbiology , Meningitis/microbiology , Metagenomics , Moraxellaceae Infections/microbiology , Psychrobacter/genetics , Adolescent , Base Sequence , Fatal Outcome , Genome, Bacterial/genetics , Humans , Male , Meningitis/cerebrospinal fluid , Mexico , Molecular Sequence Data , Moraxellaceae Infections/cerebrospinal fluid , Phylogeny , Psychrobacter/classification , Psychrobacter/isolation & purification , RNA, Ribosomal, 16S/genetics , Virulence Factors/geneticsABSTRACT
Varicella-zoster virus (VZV) is a member of the Herpesviridae family, which causes varicella (chicken pox) and herpes zoster (shingles) in humans. Here, we report the complete genome sequence of varicella-zoster virus, isolated from a vesicular fluid sample, revealing the circulation of VZV clade VIII in Mexico.
