Subject(s)
Vaccinia virus/pathogenicity , Animals , Chick Embryo , Chickens , Guinea Pigs , Rabbits , Vaccinia virus/immunology , Vaccinia virus/ultrastructureSubject(s)
Disease Outbreaks/epidemiology , Poliomyelitis/epidemiology , Child , Child, Preschool , Female , Humans , India , MaleSubject(s)
Vaccinia virus/pathogenicity , Animals , Chick Embryo , Guinea Pigs , Rabbits , Vaccinia/immunologySubject(s)
Poxviridae Infections/microbiology , Animals , Chick Embryo , Chickens , Guinea Pigs , Haplorhini , Humans , India , Monkeypox virus/growth & development , Monkeypox virus/pathogenicity , Rabbits , VirulenceSubject(s)
Poxviridae Infections/microbiology , Poxviridae/growth & development , Virus Cultivation , Animals , Chick Embryo , Chickens , Culture Techniques , Extraembryonic Membranes , Guinea Pigs , Liver , Lung , Rabbits , SpleenSubject(s)
Antigens, Viral , Smallpox Vaccine , Vaccinia virus/immunology , Animals , Freeze Drying , Immunodiffusion , Rabbits/immunology , TemperatureSubject(s)
Antibody Formation , Smallpox Vaccine , Vaccinia virus/pathogenicity , Animals , Animals, Newborn , Brain/microbiology , Chick Embryo , Drug Stability , Kidney/microbiology , Liver/microbiology , Lung/microbiology , Mice , Spleen/microbiology , Temperature , Vaccinia virus/isolation & purificationSubject(s)
Hot Temperature , Smallpox Vaccine/standards , Analysis of Variance , Animals , Biological Assay , Chick Embryo , Drug Stability , Freeze DryingABSTRACT
Comparisons were made in groups of schoolchildren in New Delhi of the rotary lancet and bifurcated needle techniques for primary vaccination and revaccination against smallpox. Vaccines of 3 different titres were employed in the tests. The results indicate that the bifurcated needle technique is superior to the rotary lancet technique for both primary vaccination and revaccination, the percentage of major reactions in revaccinations in which the bifurcated needle technique was used being significantly higher, irrespective of the vaccine dilution. However, the proportion of major reactions decreased gradually in both techniques with the greater dilution of the vaccine.It was shown that when a fully potent vaccine is used there is no advantage in making 2 insertions by the bifurcated needle technique but that when the rotary lancet technique is employed and when the vaccine is not fully potent there is a significant improvement in the successful take rate when 2 insertions are given. In areas where storage facilities for vaccines in the field are not ideal and the full potency of vaccines is doubtful, the use of the bifurcated needle technique with 2 insertions seems to be the method of choice.