Experimental Investigation on the In-Plane Creep Behavior of a Carbon-Fiber Sheet Molding Compound at Elevated Temperature at Different Stress States.

The creepage behavior of one thermosetting carbon fiber sheet molding compound (SMC) material was studied applying in-plane loading at 120 °C. Loads were applied in bending, tension and compression test setups at the same in-plane stress level of 47 MPa. Different creep strain rates were determined. The creep strain rate in flexural loading was significantly higher than in tensile loading. The test specimens in compression loading collapsed within minutes and no findings regarding the creep strain rates were possible. Overall, it was observed that the thermosetting press resin of this industrially used material had only little creep load bearing capacity at the mentioned temperature when loaded in mixed stress states. The test data has high usage for estimating design limits of structural loaded SMC components at elevated temperature.

Creep-Induced Screw Preload Loss of Carbon-Fiber Sheet Molding Compound at Elevated Temperature.

The application of chopped-fiber reinforced polymers in screwed connections at high temperatures raises the question of creep under long-term loading. While up to now thermoplastic materials have mainly been the focus of attention when it comes to creep, this paper shows that thermoset carbon-fiber SMCs (sheet mold compounds) can also be affected by this phenomenon. Screwed connections were investigated regarding their loss of preload force at 120 °C ambient temperature. Additionally, strain-time diagrams were recorded at different stress levels at 120 °C in a creep test setup of a universal testing machine by using optical strain tracking of SMC coupons. The transverse modulus under compression in thickness direction was determined in the same test setup. For data application within a FEA (finite element analysis) software power law curves according to Norton-Bailey creep law were fitted in the strain-time graphs. The applicability of the obtained creep law was crosschecked with a test carried out on the loss of preload force of a screwed connection. The developed simulative methodology offers the possibility to simulate various mounting situations of the bolted connection and to investigate measures against the loss of preload force easily. A promising possibility to limit the loss of preload force due to creep was simulatively evaluated.

Identifying Fishes through DNA Barcodes and Microarrays.

BACKGROUND: International fish trade reached an import value of 62.8 billion Euro in 2006, of which 44.6% are covered by the European Union. Species identification is a key problem throughout the life cycle of fishes: from eggs and larvae to adults in fisheries research and control, as well as processed fish products in consumer protection. METHODOLOGY/PRINCIPAL FINDINGS: This study aims to evaluate the applicability of the three mitochondrial genes 16S rRNA (16S), cytochrome b (cyt b), and cytochrome oxidase subunit I (COI) for the identification of 50 European marine fish species by combining techniques of "DNA barcoding" and microarrays. In a DNA barcoding approach, neighbour Joining (NJ) phylogenetic trees of 369 16S, 212 cyt b, and 447 COI sequences indicated that cyt b and COI are suitable for unambiguous identification, whereas 16S failed to discriminate closely related flatfish and gurnard species. In course of probe design for DNA microarray development, each of the markers yielded a high number of potentially species-specific probes in silico, although many of them were rejected based on microarray hybridisation experiments. None of the markers provided probes to discriminate the sibling flatfish and gurnard species. However, since 16S-probes were less negatively influenced by the "position of label" effect and showed the lowest rejection rate and the highest mean signal intensity, 16S is more suitable for DNA microarray probe design than cty b and COI. The large portion of rejected COI-probes after hybridisation experiments (>90%) renders the DNA barcoding marker as rather unsuitable for this high-throughput technology. CONCLUSIONS/SIGNIFICANCE: Based on these data, a DNA microarray containing 64 functional oligonucleotide probes for the identification of 30 out of the 50 fish species investigated was developed. It represents the next step towards an automated and easy-to-handle method to identify fish, ichthyoplankton, and fish products.

Interaction between two polyelectrolyte brushes.

We report molecular dynamics simulations on completely charged polyelectrolyte brushes grafted to two parallel surfaces. The pressure Pi is evaluated as a function of separation D between the two grafting planes. For decreasing separation, Pi shows several regimes distinguished by their scaling with D which reflects the different physical nature of the various regimes. At weak compression the pressure obeys the 1D power law predicted by scaling theory of an ideal gas of counterions in the osmotic brush regime. In addition we find that the brushes shrink as they approach each other trying to avoid interpenetration. At higher compressions where excluded volume interactions become important, we obtain scaling exponents between -2 at small grafting density rho(a) and -3 at large rho(a). This behavior indicates a transition from a brush under good solvent condition to the melt regime with increasing grafting density.