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1.
Inflammation ; 38(4): 1401-5, 2015 Aug.
Article in English | MEDLINE | ID: mdl-25633424

ABSTRACT

The half-lives of typical acute phase proteins in rats and beagle dogs during acute inflammation were investigated. Acute inflammation was induced by injection of turpentine oil in rats and administration of indomethacin in beagle dogs. Serum concentrations of α2-macroglobulin (α2M) and C-reactive protein (CRP) were measured by enzyme-linked immunosorbent assay and α1-acid glycoprotein (AAG) was measured by single radial immunodiffusion. Half-life was calculated as 0.693/elimination rate constant (K). The mean half-lives in the terminal elimination phase of α2M and AAG were 68.1 and 164.8 h, respectively. The half-life of AAG was significantly longer than that of α2M. Mean half-lives in the terminal elimination phase of CRP and AAG were 161.9 and 304.4 h, respectively. The half-life of AAG was significantly longer than that of CRP in beagle dogs. No significant differences in the half-life of AAG were observed between rats and beagle dogs. Furthermore, serum concentrations in the terminal elimination phase could be simulated with the K data acquired in this study.


Subject(s)
Acute-Phase Proteins/metabolism , Acute-Phase Reaction/blood , C-Reactive Protein/metabolism , Orosomucoid/metabolism , alpha-Macroglobulins/metabolism , Acute-Phase Reaction/pathology , Animals , Dogs , Half-Life , Inflammation/blood , Inflammation/pathology , Male , Rats , Rats, Sprague-Dawley , Species Specificity
2.
Inflammation ; 36(6): 1448-52, 2013 Dec.
Article in English | MEDLINE | ID: mdl-23856939

ABSTRACT

Synthesis of α2-macoglobulin (α2M) by 3-week-old juvenile rats was compared to that of mature 7- and 11-week-old rats. Serum concentrations of α2M, interleukin (IL)-6- and cytokine-induced neutrophil chemoattractant (CINC)-1 were measured by enzyme-linked immunosorbent assay. The area under the concentration vs. time curve (AUC) for α2M was significantly different among the three groups. The synthesis of α2M increased in an age-dependent manner. No significant difference was observed for the AUC of IL-6, but that of CINC-1 in 3-week-old rats was significantly lower than that in 7- or 11-week-old rats. These results suggest that synthesis of α2M was increased in mature compared to juvenile rats, possibly due to differences in liver function. The maximum concentration of CINC-1 in 3-week-old rats was observed 6 h after turpentine oil injection. The serum concentrations of IL-6 and CINC-1 increased more quickly in juvenile rats than in mature rats after inflammatory stimulation.


Subject(s)
Acute-Phase Reaction/blood , Chemokine CXCL1/blood , Inflammation/blood , Interleukin-6/blood , alpha-Macroglobulins/biosynthesis , Age Factors , Animals , Chemokine CXCL1/biosynthesis , Disease Models, Animal , Inflammation/metabolism , Interleukin-6/biosynthesis , Male , Rats , Rats, Sprague-Dawley , Turpentine
3.
Comp Med ; 59(2): 163-7, 2009 Apr.
Article in English | MEDLINE | ID: mdl-19389308

ABSTRACT

A bovine colostral antibody against verotoxin (VT) 2 of Escherichia coli O157:H7 was administered orally to beagle dogs. The antibody remained in the dogs' small intestine for at least 2 h, whereas little serum antibody remained 1.5 h after administration. Furthermore, the antibody activity of secretory IgA did not change until 2 h after administration; however, the activity of IgG and IgM antibodies decreased by approximately 60% and 40% at 2 h after administration, respectively. Seven beagle dogs inoculated with Escherichia coli O157:H7 producing VT2 were administered bovine colostral antibody or bovine colostral whey without antibody. With administration of bovine colostral whey without antibody, the amount of VT2 in feces decreased gradually after administration and increased again at 5 d after inoculation, whereas bovine colostral antibody significantly reduced the amount of VT2 in feces on the day after administration. In addition, 9 beagle dogs were given bovine colostral antibody, bovine plasma antibody, or saline. The amount of VT2 in feces again decreased significantly more rapidly after administration of bovine colostral antibody than after administration of bovine plasma antibody or saline.


Subject(s)
Antibodies/immunology , Colostrum/immunology , Escherichia coli O157/immunology , Peptide Hydrolases/metabolism , Shiga Toxin 2/immunology , Animals , Antibodies/administration & dosage , Cattle , Colostrum/chemistry , Dogs , Escherichia coli O157/pathogenicity , Female , Humans , Immunoglobulins/immunology , Male , Pregnancy
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