1.
Mol Biosyst
; 8(7): 1861-4, 2012 Jul 06.
Article
in English
| MEDLINE
| ID: mdl-22627921
ABSTRACT
The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS-DNA complexes and will enable their structural and functional analysis.