ABSTRACT
The serine/threonine kinase PAK4 is a Cdc42 effector whose role is not well understood; overexpression of PAK4 has been associated with some cancers, and there are reports that correlate kinase level with increased cell migration in vitro. Here we report that PAK4 is primarily associated with cell-cell junctions in all the cell lines we tested, and fails to accumulate at focal adhesions or at the leading edge of migrating cells. In U2OS osteosarcoma and MCF-7 breast cancer cell lines, PAK4 depletion did not affect collective cell migration, but affected cell polarization. By contrast, Cdc42 depletion (as reported by many studies) caused a strong defect in junctional assembly in multiple cells lines. We also report that the depletion of PAK4 protein or treatment of cells with the PAK4 inhibitor PF-3758309 can lead to defects in centrosome reorientation (polarization) after cell monolayer wounding. These experiments are consistent with PAK4 forming part of a conserved cell-cell junctional polarity Cdc42 complex. We also confirm ß-catenin as a target for PAK4 in these cells. Treatment of cells with PF-3758309 caused inhibition of ß-catenin Ser-675 phosphorylation, which is located predominantly at cell-cell junctions.
Subject(s)
Cell Polarity , Intercellular Junctions/metabolism , cdc42 GTP-Binding Protein/metabolism , p21-Activated Kinases/metabolism , Cell Line, Tumor/drug effects , Cell Polarity/drug effects , Gene Knockdown Techniques , Humans , Intercellular Junctions/drug effects , MCF-7 Cells/drug effects , Phosphorylation , Protein Kinase Inhibitors/pharmacology , Pyrazoles/pharmacology , Pyrroles/pharmacology , Serine/metabolism , beta Catenin/metabolism , cdc42 GTP-Binding Protein/genetics , p21-Activated Kinases/antagonists & inhibitors , p21-Activated Kinases/geneticsABSTRACT
p21-activated kinases (PAKs) are Cdc42 effectors found in metazoans, fungi and protozoa. They are subdivided into PAK1-like (group I) or PAK4-like (group II) kinases. Human PAK4 is widely expressed and its regulatory mechanism is unknown. We show that PAK4 is strongly inhibited by a newly identified auto-inhibitory domain (AID) formed by amino acids 20 to 68, which is evolutionarily related to that of other PAKs. In contrast to group I kinases, PAK4 is constitutively phosphorylated on Ser 474 in the activation loop, but held in an inactive state until Cdc42 binding. Thus, group II PAKs are regulated through conformational changes in the AID rather than A-loop phosphorylation.
Subject(s)
cdc42 GTP-Binding Protein/metabolism , p21-Activated Kinases/chemistry , p21-Activated Kinases/metabolism , Amino Acid Sequence , Animals , Binding Sites , COS Cells , Chlorocebus aethiops , Enzyme Activation , Molecular Sequence Data , Mutation , Phosphorylation , Serine/metabolism , p21-Activated Kinases/geneticsABSTRACT
Translocated in liposarcoma (TLS or FUS) is a multifunctional protein component of the heterogenous ribonuclear complex involved in the splicing of pre-mRNA and the export of fully processed mRNA from the nucleus to the cytoplasm. As we determined that TLS was substantially expressed in the adult retina, we investigated the functions of TLS in a rat retinal ganglion cell (RGC) line RGC-5. TLS was found to be associated with N-methyl-d-aspartate (NMDA) receptor 1 (NR1) and myosinVa (MyoVa) in a calcium-dependent manner. We demonstrated that TLS-associated NR1 could be one of the NR1 alternative splice variants, NR1-4, which was predominantly expressed in RGC-5. The degree of colocalization between TLS and NR1 was significantly decreased by depolarization of RGC-5 cells, indicating that the depolarization-induced Ca(2+)-influx triggered a redistribution of NR1 from the TLS-protein complex. These results suggested that TLS might be involved in a calcium-dependent trafficking of specific NR1 splice variants in RGCs.
