Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Language
Publication year range
1.
Curr Biol ; 11(20): 1578-85, 2001 Oct 16.
Article in English | MEDLINE | ID: mdl-11676917

ABSTRACT

BACKGROUND: A fundamental challenge of evolutionary and developmental biology is understanding how new characters arise and change. The recently derived eyespots on butterfly wings vary extensively in number and pattern between species and play important roles in predator avoidance. Eyespots form through the activity of inductive organizers (foci) at the center of developing eyespot fields. Foci are the proposed source of a morphogen, the levels of which determine the color of surrounding wing scale cells. However, it is unknown how reception of the focal signal translates into rings of different-colored scales, nor how different color schemes arise in different species. RESULTS: We have identified several transcription factors, including butterfly homologs of the Drosophila Engrailed/Invected and Spalt proteins, that are deployed in concentric territories corresponding to the future rings of pigmented scales that compose the adult eyespot. We have isolated a new Bicyclus anynana wing pattern mutant, Goldeneye, in which the scales of one inner color ring become the color of a different ring. These changes correlate with shifts in transcription factor expression, suggesting that Goldeneye affects an early regulatory step in eyespot color patterning. In different butterfly species, the same transcription factors are expressed in eyespot fields, but in different relative spatial domains that correlate with divergent eyespot color schemes. CONCLUSIONS: Our results suggest that signaling from the focus induces nested rings of regulatory gene expression that subsequently control the final color pattern. Furthermore, the remarkably plastic regulatory interactions downstream of focal signaling have facilitated the evolution of eyespot diversity.


Subject(s)
Butterflies/genetics , Homeodomain Proteins/genetics , Pigmentation/genetics , Transcription Factors/genetics , Wings, Animal/physiology , Animals , Biological Evolution , Butterflies/growth & development , Drosophila Proteins , Genetic Variation/genetics , Homeodomain Proteins/metabolism , Mutation/genetics , Pupa/growth & development , Pupa/metabolism , Sequence Homology , Transcription Factors/metabolism , Wings, Animal/growth & development
2.
Curr Biol ; 9(22): 1279-87, 1999 Nov 18.
Article in English | MEDLINE | ID: mdl-10574759

ABSTRACT

BACKGROUND: The morphological diversity of arthropods makes them attractive subjects for studying the evolution of developmental mechanisms. Comparative analyses suggest that arthropod diversity has arisen largely as a result of changes in expression patterns of genes that control development. Direct analysis of how a particular gene functions in a given species during development is hindered by the lack of broadly applicable techniques for manipulating gene expression. RESULTS: We report that the Arbovirus Sindbis can be used to deliver high levels of gene expression in vivo in a number of non-host arthropod species without causing cytopathic effects in infected cells or impairing development. Using recombinant Sindbis virus, we investigated the function of the homeotic gene Ultrabithorax in the development of butterfly wings and beetle embryos. Ectopic Ultrabithorax expression in butterfly forewing imaginal discs was sufficient to cause the transformation of characteristic forewing properties in the adult, including scale morphology and pigmentation, to those of the hindwing. Expression of Ultrabithorax in beetle embryos outside of its endogenous expression domain affected normal development of the body wall cuticle and appendages. CONCLUSIONS: The homeotic genes have long been thought to play an important role in the diversification of arthropod appendages. Using recombinant Sindbis virus, we were able to investigate homeotic gene function in non-model arthropod species. We found that Ultrabithorax is sufficient to confer hindwing identity in butterflies and alter normal development of anterior structures in beetles. Recombinant Sindbis virus has broad potential as a tool for analyzing how the function of developmental genes has changed during the diversification of arthropods.


Subject(s)
DNA-Binding Proteins/biosynthesis , Drosophila Proteins , Gene Expression Regulation, Developmental , Gene Expression Regulation, Viral , Genes, Homeobox , Genetic Vectors/genetics , Homeodomain Proteins/biosynthesis , Sindbis Virus/genetics , Transcription Factors , Animals , Artemia/embryology , Artemia/genetics , Butterflies/growth & development , Butterflies/ultrastructure , Cytopathogenic Effect, Viral , DNA-Binding Proteins/genetics , Drosophila melanogaster/genetics , Head/embryology , Hemiptera/embryology , Hemiptera/genetics , Homeodomain Proteins/genetics , Larva , Morphogenesis/genetics , Organ Specificity , Pigmentation/genetics , Pupa , Recombinant Fusion Proteins/analysis , Recombination, Genetic , Species Specificity , Thorax/embryology , Tribolium/embryology , Tribolium/ultrastructure , Wings, Animal/ultrastructure
3.
Science ; 283(5401): 532-4, 1999 Jan 22.
Article in English | MEDLINE | ID: mdl-9915699

ABSTRACT

The origin of new morphological characters is a long-standing problem in evolutionary biology. Novelties arise through changes in development, but the nature of these changes is largely unknown. In butterflies, eyespots have evolved as new pattern elements that develop from special organizers called foci. Formation of these foci is associated with novel expression patterns of the Hedgehog signaling protein, its receptor Patched, the transcription factor Cubitus interruptus, and the engrailed target gene that break the conserved compartmental restrictions on this regulatory circuit in insect wings. Redeployment of preexisting regulatory circuits may be a general mechanism underlying the evolution of novelties.


Subject(s)
Butterflies/genetics , Drosophila Proteins , Gene Expression Regulation , Insect Proteins/genetics , Wings, Animal/growth & development , Animals , Biological Evolution , Body Patterning , Butterflies/anatomy & histology , Butterflies/growth & development , DNA-Binding Proteins/genetics , DNA-Binding Proteins/physiology , Genes, Insect , Hedgehog Proteins , Homeodomain Proteins/genetics , Homeodomain Proteins/physiology , Insect Proteins/physiology , Membrane Proteins/genetics , Membrane Proteins/physiology , Pigmentation , Receptors, Cell Surface , Signal Transduction , Transcription Factors/genetics , Transcription Factors/physiology , Transcription, Genetic , Wings, Animal/anatomy & histology , Wings, Animal/metabolism
4.
Science ; 265(5168): 109-14, 1994 Jul 01.
Article in English | MEDLINE | ID: mdl-7912449

ABSTRACT

Butterfly wings display pattern elements of many types and colors. To identify the molecular processes underlying the generation of these patterns, several butterfly cognates of Drosophila appendage patterning genes have been cloned and their expression patterns have been analyzed. Butterfly wing patterns are organized by two spatial coordinate systems. One system specifies positional information with respect to the entire wing field and is conserved between fruit flies and butterflies. A second system, superimposed on the general system and involving several of the same genes, operates within each wing subdivision to elaborate discrete pattern elements. Eyespots, which form from discrete developmental organizers, are marked by Distal-less gene expression. These circular pattern elements appear to be generated by a process similar to, and perhaps evolved from, proximodistal pattern formation in insect appendages.


Subject(s)
Butterflies/genetics , Drosophila Proteins , Gene Expression Regulation , Genes, Insect , Homeodomain Proteins , Photoreceptor Cells, Invertebrate/growth & development , Wings, Animal/growth & development , Amino Acid Sequence , Animals , Base Sequence , Butterflies/embryology , Butterflies/growth & development , DNA, Complementary/genetics , Drosophila/genetics , Genes, Homeobox , Insect Hormones/chemistry , Insect Hormones/genetics , LIM-Homeodomain Proteins , Molecular Sequence Data , Proto-Oncogene Proteins/chemistry , Proto-Oncogene Proteins/genetics , Transcription Factors/chemistry , Transcription Factors/genetics , Transcription, Genetic , Wnt1 Protein
5.
J Immunol ; 142(11): 3971-7, 1989 Jun 01.
Article in English | MEDLINE | ID: mdl-2497184

ABSTRACT

We have established long term cell lines from a patient with adenosine deaminase (ADA)-deficient severe combined immunodeficiency by stimulation of blood and bone marrow cells with PHA and IL-2 followed by transformation of the activated cells with the human retrovirus HTLV-I. Despite the absence of detectable T cells in the patients blood, cell lines grew that carried the phenotype of mature activated T cells. TJF-2, the line established from blood, was characterized in detail. The concentration of ADA in TJF-2 cells was less than 1% of normal (3.2 U vs 413.0 U). Studies with pharmacologic inhibitors of ADA suggest that the residual adenosine deaminating activity of TJF-2 is from an enzyme distinct from true ADA, a nonspecific aminohydrolyase. Growth of TJF-2 cells was hypersensitive to inhibition by 2'-deoxyadenosine compared to normal T cells (ID50, 55 microM vs greater than 1000 microM). Analysis of 2'-deoxyadenosine-challenged cells showed that TJF-2 cells accumulated significant levels of deoxyadenosine triphosphate, whereas normal T cells did not unless they were also incubated with the ADA inhibitor deoxycoformycin. Southern and Northern blot analysis of these cells revealed a grossly intact ADA gene that produced a normal size ADA mRNA. Yet, despite ADA deficiency, cells of the TJF-2 line were otherwise indistinguishable from HTLV-I-transformed T cells derived from normal donors with respect to dependence on exogenous IL-2 for growth, clonal rearrangement patterns of TCR beta-chain genes, response to PHA, and rapid restoration of cellular volume after hypotonic challenge. The TJF-2 line thus represents a unique HTLV-I-transformed human T cell line exhibiting ADA deficiency and its expected metabolic consequences.


Subject(s)
Adenosine Deaminase/deficiency , Immunologic Deficiency Syndromes/enzymology , Nucleoside Deaminases/deficiency , T-Lymphocytes/enzymology , Adenosine Deaminase/genetics , Adenosine Deaminase Inhibitors , Cell Line , Cell Line, Transformed , Cell Transformation, Viral , Child, Preschool , Deoxyadenine Nucleotides/metabolism , Deoxyadenosines/pharmacology , Human T-lymphotropic virus 1 , Humans , Hypotonic Solutions , Immunoglobulin Heavy Chains/genetics , Immunologic Deficiency Syndromes/genetics , Immunologic Deficiency Syndromes/immunology , Interleukin-2/biosynthesis , Interleukin-2/pharmacology , Lymphocyte Activation/drug effects , Male , Phytohemagglutinins , RNA, Messenger/isolation & purification , Receptors, Antigen, T-Cell/genetics , T-Lymphocytes/immunology , T-Lymphocytes/metabolism
6.
Proc Natl Acad Sci U S A ; 83(20): 7790-4, 1986 Oct.
Article in English | MEDLINE | ID: mdl-3094015

ABSTRACT

Autoradiography of restriction digests of DNA labeled in early S phase indicates that replication of the amplified dihydrofolate reductase (DHFR) domain of methotrexate-resistant CHOC 400 cells initiates within a 6.1-kilobase pair (kb) EcoRI-doublet located on the 3' side of the DHFR gene. To localize the DHFR origin fragment, synchronized CHOC 400 cells were either pulse labeled with [3H]thymidine in vivo or permeabilized and incubated with [32P]dATP under conditions that support limited chromosomal DNA replication. The temporal order of replication of amplified fragments was determined by hybridization of the in vivo or in vitro replication products to cloned fragments spanning the earliest-replicating portion of the DHFR domain. At the G1/S boundary, the labeled products derived from the replication of amplified sequences, either in whole or permeabilized cells, are distributed about an amplified 4.3-kb Xba I fragment that maps 14 kb downstream from the DHFR gene. As cells progress through the S phase, bidirectional replication away from this site is observed. These studies indicate that the 4.3-kb Xba I fragment contains the origin of replication associated with the amplified DHFR domain.


Subject(s)
DNA Replication , Gene Amplification , Tetrahydrofolate Dehydrogenase/genetics , Aphidicolin , Cells, Cultured , DNA/analysis , Diterpenes/pharmacology , Nucleic Acid Hybridization , Permeability
7.
Biochemistry ; 25(2): 441-9, 1986 Jan 28.
Article in English | MEDLINE | ID: mdl-3006761

ABSTRACT

1-beta-D-Arabinofuranosylcytosine (ara-C) inhibits nuclear DNA replication in Chinese hamster ovary cells by an efficient chain termination mechanism without affecting the rate at which cells traverse G1 and enter S [Heintz, N. H., & Hamlin, J. L. (1983) Biochemistry 22, 3557-3562]. Here we have employed ara-C to enrich for replication intermediates formed during initiation of DNA synthesis in synchronized CHOC 400 cells, a methotrexate-resistant derivative of Chinese hamster ovary cells that contains approximately 1000 copies of an early replicating 150-kb chromosomal domain. This highly amplified domain includes the gene for dihydrofolate reductase (DHFR). CHOC 400 cells were collected at the G1/S boundary of the cell cycle with aphidicolin prior to release into S in the presence of both [methyl-3H] thymidine and various concentrations of ara-C. Chromatographic fractionation of restriction endonuclease digests over benzoylated naphthoylated DEAE-cellulose (BND-cellulose) showed that high concentrations of ara-C inhibited the maturation of chromosomal replication intermediates containing ssDNA (replication forks) into dsDNA for up to 60 min. The effect of ara-C on the sequence complexity of replication intermediates formed during early S phase was determined by hybridizing purified intermediates labeled with 32P in vitro to Southern blots of genomic DNA derived from both methotrexate-sensitive and methotrexate-resistant Chinese hamster ovary cells. In the absence of ara-C, 32P-labeled ssDNA BND-cellulose fractions from cultures released into S for 30-60 min hybridized to a spectrum of restriction fragments encompassing 40-50 kb of the amplified DHFR domain.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
DNA Replication , Genes/drug effects , Methotrexate/pharmacology , Tetrahydrofolate Dehydrogenase/genetics , Animals , Base Sequence , Cell Cycle/drug effects , Cell Line , Cricetinae , Cricetulus , Cytarabine/pharmacology , DNA/isolation & purification , DNA Restriction Enzymes , Drug Resistance , Female , Gene Amplification , Hydroxyurea/pharmacology , Kinetics , Ovary
SELECTION OF CITATIONS
SEARCH DETAIL
...