ABSTRACT
Streptococcus suis bacteria are one of the most serious health problems for pigs and an emerging zoonotic agent in humans working in the swine industry. S.â suis bacteria express capsular polysaccharides (CPS) a major bacterial virulence factor that define the serotypes. Oligosaccharides resembling the CPS of S. suis serotypes 2, 3, 9, and 14 have been synthesized, glycans related to serotypes 2 and 9 were placed on glycan array surfaces to screen blood from infected pigs. Lead antigens for the development of semi-synthetic S. suis serotypes 2 and 9 glycoconjugate veterinary vaccines were identified in this way.
Subject(s)
Anti-Bacterial Agents/pharmacology , Antigens/immunology , Glycoconjugates/pharmacology , Polysaccharides, Bacterial/immunology , Streptococcus suis/drug effects , Vaccines, Synthetic/pharmacology , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/immunology , Antigens/chemistry , Drug Discovery , Glycoconjugates/chemistry , Glycoconjugates/immunology , Polysaccharides, Bacterial/chemistry , Vaccines, Synthetic/chemistry , Vaccines, Synthetic/immunologyABSTRACT
Streptococcus pneumoniae causes life-threatening diseases including meningitis, pneumonia and sepsis. Existing glycoconjugate vaccines based on purified capsular polysaccharides are widely used and help to prevent millions of deaths every year. Herein, the total syntheses of oligosaccharides resembling portions of the S. pneumoniae serotype 7F (ST7F) capsular polysaccharide repeating unit are reported. To define minimal glycan epitopes, glycan microarrays containing the synthetic oligosaccharides were used to screen human reference serum and revealed that both side chains of the ST7F play a key role in antigen recognition. The identification of protective minimal epitopes is vital to design efficient semi- and fully-synthetic glycoconjugate vaccines.
