[Blood levels of micro- and microelements in aged patients suffering from chronic coronal heart disease]. / Makro- i mikroélementy krovi u patsientov pozhilogo i starcheskogo vozrasta, stradaiushchikh khronicheskoi bolezn'iu serdtsa.

The authors measured serum and whole blood levels of macro- and microelements (ME), such as iron, zinc, sulfur, potassium, calcium in 149 aged patients, suffering from coronary heart disease. The levels of the above ME were measured in various age groups: younger than 60, 60 to 74, 80 to 89 and older than 90 years old. In 36 aged women ME levels were established in correlation with bone mineral density.

[Treatment of purulent-inflammatory complications of polytrauma, diabetes, infected pancreonecrosis]. / Lechenie gnoino-vospalitel'nykh oslozhnenii, soprovazhdaiushchikh politravmu, sakharnyi diabet, infitsirovannyi pankreonekroz.

Results of Antilympholin-K3 use in combined treatment of purulent-inflammatory complications in polytrauma, diabetes, infected pancreonecrosis are analysed. Thirty-nine patients aged from 19 to 74 years were treated. Double injection of Antilympholin-K3 enhanced phagocytic activity of neutrophils, phagocytic index, chemiluminescence of mononuclears and neutrophils, immunoglobulins concentration. Positive dynamics in immunological indices is accompanied by improving clinical status, cleaning of postoperative wounds from pus, improving of epithelisation.

[Immune and interferon system in patients with bronchial asthma]. / Sostoianie immunnoi i interferonovoi sistem u bol'nykh bronkhial'noi astmoi.

We studied the levels of acute inflammation phase proteins (AIPP), production of alpha, gamma and serum interferons, the activity of epsilon-receptors of T-lymphocytes in 39 patients with bronchial asthma (BA). Correlations were made with the process severity and basic therapy. We discovered imbalance of immunoregulatory mechanisms: enhanced AIPP synthesis, low production of induced alpha and gamma interferons, elevated concentrations of poor-differentiated T-lymphocytes. This confirms persisting inflammation in BA.

[The methodological characteristics of determining the basic and trace elements in the serum and peripheral blood cells by the x-ray fluorescence method]. / Metodicheskie osobennosti opredeleniia osnovnykh i mikroélementov v syvorotke i kletkakh perifericheskoi krovi rentgenofliuorestsentnym metodom.

Analytical parameters of instrumental methods of the element analysis of biomedical objects are compared and the place occupied by x-ray fluorescent analysis (RFA) among them is demonstrated. The methods for preparing the specimens of liquid media of the organism and the RFA are simple, rapid, involve no disintegration of the sample, and allow measurements of elements at sigma 1 = 0.02 at concentrations of 12 micrograms/ml. The method is preferable and promising for some basic elements (P, S, Cl, K, Ca), which are difficult to measure by other methods. The levels of S, K, Ca, Fe, Zn, and Cu were measured in erythrocytic mass and blood serum of normal subjects, patients with asthma and aplastic anemia, and recipients of allogenic kidneys.

[Effect of low intensity laser radiation with various wavelength on bone marrow immunopoiesis progenitors]. / Vliianie malointensivnogo lazernogo izlucheniia razlichnykh dlin voln na kostno-mozgovye predshestvenniki immunopoéza.

Effects of helium-neon and cadmium-neon lasers with wavelength 632.8 and 470 nm correspondingly with power 20 mw and also argon laser with wavelength 480 nm, power 1 w on "early" and "late" colony-forming units (CFU) of mice bone marrow were appreciated. The helium-neon laser as compared to the cadmium-neon one stimulated the "late" CFU and incorporation of 3H-thymidine in DNA of the bone marrow cells after 30 or 45 min exposition. The more powerful argon laser after 30 min exposition suppressed "early" and "late" CFU completely by 50% dead cells of the bone marrow. A decrease of the argon laser exposition to 5 min was accompanied, by the preservation of the bone marrow cells viability at the control level and ability to stimulate "early" CFU.

[The effect of the bronchoalveolar fluid of mice infected with the influenza virus on hematopoietic bone marrow precursors]. / Vliianie bronkhoal'veoliarnoi zhidkosti myshei, infitsirovannykh virusom grippa, na kostnomozgovye predshestvenniki gemopoéza.

The influence of bronchoalveolar washing fluid (BAWF), as well as BAWF cells, obtained from mice infected with influenza virus, on the formation of exogenic spleen colony-forming units (CFUs) of lethally irradiated syngeneic recipients was studied. BAWF and BAWF cells of intact syngeneic mice stimulated the growth of CFUs. BAWF of mice infected with nonpathogenic strain A/PR/8/34 lost its capacity for stimulating the growth of colonies, and BAWF cells greatly suppressed colony formation in the spleen of recipients. The participation of interferon, colony-stimulating factor and the virus itself in the process of the modulation of colony formation is discussed.

[The infection of mice with the influenza virus suppresses bone marrow precursor differentiation in the erythroid and granulocyte directions]. / Infitsirovanie myshei virusom grippa ugnetaet differentsirovku kostnomozgovykh predshestvennikov v éritroidnom i granulotsitarnom napravleniiakh.

Mice (CBA X C57BL/6)F1 were inoculated intranasally with nonlethal doses of influenza A/PR/8/34 virus (500 EID50). At different intervals postinoculation, bone marrow cells were collected from them and inoculated intravenously to lethally irradiated syngeneic recipients with the purpose of determination of the histological types of colony-forming units of spleen (CFUs). On day 3 after inoculation, inhibition of CFUs differentiation in erythroid and granulocytic directions was observed, and in 7 days only granulocytic shoot of hemopoiesis was inhibited. Lymph node cells of the donors in 3 days postinoculation inhibited the colony-forming activity of bone marrow cells of intact mice with suppression of differentiation of erythrocytes and granulocytes. The possible mechanisms of influenza virus-mediated effect on the processes of bone marrow hemopoiesis are discussed.

[Effects of burn injury on the reactivity of spleen cells of mice of different genotypes during regional graft vs host reaction]. / Vliianie ozhogovoi travmy na reaktivnost' kletok selezenki myshei raznykh genotipov v regionarnoi reaktsii transplantat protiv khoziaina.

Different strains of mice CBA (H-2k) and C57BL/6 (H-2b) have been exposed to burn of flame of 15% skin-deep of body. Spleen cells were received 6 hours, 3 and 7 days after burn trauma of mice in order to induce the local graft-versus-host reaction by subcutaneous injection of 10(7) cells in foot of hind leg of (CBA X C57BL/6)F1 hybrids. The enhancement of the ability of spleen cells to induce graft-versus host was observed in 6 hours and 3 days. The burn trauma of C57BL/6 mice produced the suppression of reactivity of spleen cells in graft-versus-host reaction for all terms of research. The donors of spleen cells adrenalectomy 5 days before the burn trauma eliminated differences of reactivity of lymphocytes of mice CBA and C57BL/6.

The influence of endogenous hypocorticism on the replication of influenza virus in the mouse lungs and the production of specific antibodies.

The replication of pathogenic influenza virus A/PR/8/34 in the lungs and the synthesis of virus-neutralizing (VN) and haemagglutination-inhibition (HI) antibodies has been studied in mice with endogenous hypocorticism induced by a bilateral adrenalectomy. The adrenalectomized mice appeared to be more susceptible to influenza infection as compared to the mock-operated ones. This was evident from earlier deaths and higher death rate in mice inoculated with 50 EID50, 1000 EID50, and 6000 EID50 of the virus, respectively. A tendency towards decrease of specific antibody titres and the resistance to reinfection with influenza virus A/PR/8/34 was also observed.

[Genetic differences of antigens controlled by Y chromosome ensure the inactivation of bone marrow stem cells by syngeneic murine lymphocytes]. / Geneticheskie razlichiia po antigenam, kontroliruemym Y-khromosomoi, obespechivaiut inaktivatsiiu stvolovykh kletok kostnogo mozga singennymi limfotsitami myshei.

Expression of the inactivation effect of the stem colony-forming marrow cells of male mice CBA, C57B1/6, (CBA.C57B1/6)F1 and (C57B1/6.CBA)F1 by syngeneic lymphocytes of lymphatic nodes of females in conditions of transplantation of cell mixtures into lethally irradiated recipients was studied. The maximum inactivation effect of stem cells (86.3%) is determined in mice F1 with differences of H-Y antigen, the minimum effect (2.4%)--in mice CBA, an intermediate effect (54%) being registered in mice C57B1/6. Sensibility of target cells to the inactivation influence of lymphocytes distinguished by weak transplantation H-Y antigen depended on the maternal effect and direction of reaction. The inactivation effect was maximal in the combination CBA----C57B1/6 and C57B1/6----C57B1/6, as compared with the combination CBA----CBA and C57B1/6----CBA. Possible mechanisms by which the killer action of syngeneic lymphocytes on the antigen H-Y of target cells is developed differently, are under discussion.

[Suppressive action of the influenza virus on the bone marrow stem cells of mice]. / Supressivbnoe deistvie virusa grippa na stvolovye kletki kostnogo mozga myshei.

The effect of pathogenic and noninfectious influenza viruses with different surface antigens on colony-forming stem cells of (CBA X C57BL/6)F1 mice was studied. Bone marrow cells were infected with A/PR8/34 (H1N1)/A/Krasnodar/101/59 (H2N2) viruses, a recombinant A/PK-6-3 (H2N1) strain thereof, A/Khabarovsk/933/77 (H1N1) strain; then they were inoculated intravenously to irradiated (820 rad) syngeneic recipients, and colony-forming units (CFU) were determined in the spleens 9 days after injection. Inoculation of bone marrow cells (2 X 10(5) per mouse) to the irradiated recipients immediately after mixing with a live A/Krasnodar virus heated at 56 degrees C, live A/PK-6-3, and A/Khabarovsk viruses resulted in suppression of formation of CFU and marked atrophy of the recipients' spleens. There was no suppression after the use in these experiments of the pathogenic A/PR/8/34 strain. Inhibition of CFU production in the spleen was observed after inoculation of the recipients with bone marrow cells incubated for 1 hour at 37 degrees C with A/PR/8/34, A/Krasnodar/101/59 viruses and the recombinant strain. Possible mechanisms of the suppressive effect of influenza A virus on CFU production in mice are discussed.

[Humoral immune response to the influenza virus: the fractionation of sera from immune animals]. / Gumoral'nyi immunnyi otvet k virusu grippa: fraktsionirovanie syvorotok immunnykh zhivotnykh.

The studies demonstrated that antibody synthesis under conditions of the investigated fatal influenza infection differs in certain parameters from that in non-fatal infection. The mouse-pathogenic A/PR8/34 strain of influenza virus actively induced synthesis of antibodies detectable both by HI and NT. The less pathogenic A/Krasnodar/101/59 strain induced synthesis of antibodies detectable by NT sufficiently well but was a poor inducer of antibodies inhibiting virus hemagglutination of chick erythrocytes. Antibodies detectable by HI and NT appear to represent different molecules of immunoglobulins which differ in their immunochemical properties. These antibodies could be separated by means of affinity chromatography on an immunosorbent. The results of the above studies confirm that the protective and virus-neutralizing activity of an immune preparation in passive immunization is determined by the qualitative and quantitative composition of antibodies in a given preparation. The ratio of virus-induced antibodies may possibly determine the severity of the course and outcome of primary influenza infection.

[Cytotoxic activity of normal killers and the lymphocytic proliferative response to specific viral antigens in influenza in mice]. / Tsitotoksicheskaia aktivnost' normal'nykh killerov i proliferativnyi otvet limfotsitov na spetsificheskie virusnye antigeny pri grippoznoi infektsii u myshei.

Inoculation of CBA mice with pathogenic influenza A/PR8/34 (H1N1) virus and non-pathogenic A/Krasnodar/101/59 (H2N2) virus demonstrated that the pathogenic strain enhanced the synthesis of serum interferon and activated the function of normal killers, but had a relatively low capacity of causing in vitro proliferative response of spleen lymphocytes of intact and in vivo primed animals. In contrast, the nonpathogenic virus had less marked interferon-inducing capacity and that of activating normal killers, but induced a very high proliferative response of lymphocytes in culture.

[Effect of the host's body genotype and phenotype on the manifestation of the pathogenic properties of the influenza virus]. / Vliianie genotipa i fenotipa organizma khoziaina na proiavlenie patogennykh svoistv virusa grippa.

The sensitivity of mice of CBA (H-2k haplotype), C57BL/6 (H-2b haplotype) strains and their hybrids M1 and F2 to the pathogenic influenza A/PR8/34 (H0N1) and nonpathogenic A/Krasnodar/101/59 (H2N2) virus strains was studied. The lethality, virus replication in the lungs, and the thymic index were determined. An increase in the resistance to the pathogenic influenza virus was found in male C57BL/6 and male F1 (CBA X C57BL/6) hybrids in the summer period as compared with winter. Replication of the pathogenic virus in the lungs of mice, in contrast to the nonpathogenic one, was accompanied by marked atrophy of the thymus. The study suggests the existence of a certain association between the resistance to influenza infection and phenotypic changes in the host connected with the weight of the thymus and the presence of mature T-lymphocytes.

[Effect of adrenalectomy of the recipients of allogeneic lymphocytes on the inactivation of endogenous colony-forming cells in mice]. / Vliianie adrenaléktomii retsipientov allogennykh limfotsitov na inaktivatsiiu éndogennykh kolonieobrazuiushchikh kletok u myshei.

Intravenous injection of lymph node cells from the parental C57BL/6 mouse line in doses of 2 X 10(6) or 5 X 10(6) into sublethally irradiated (CBA X C57BL/6) F1 hybrids produced more demonstrable suppression of endogenous colony-formation in adrenalectomized recipients as compared with that seen in sham-operated on ones (P less than 0.01). The recipients' adrenalectomy itself was accompanied by an over 2-fold increase in the number of the endogenous colony-forming cells in the spleen as compared with sham-operated on mice. Possible mechanisms, by which the killer action of lymphocytes on the endogenous colony-forming cells is potentiated, are under discussion.