ABSTRACT
By fluorescent spectroscopy and microscopy methods the possibility of fluorescent probes DSM, E-176, 3-DAB and FME application for study of cryoprotective agents' influence on the dog spermatozoa are investigated. It is established that FME and 3-DAD dyes are suitable for the posed problem solving, and the DSM and E-176 probes have restrictions owing to enough strong fluorescence from cryoprotectant solutions. It is shown that the fluorescent probes investigated influence the cells motility to different degree. The perspectives of investigated dyes application for study of cryoprotective agent' finfluence on spermatozoa are considered.
Subject(s)
Cryoprotective Agents/pharmacology , Fluorescent Dyes , Spectrometry, Fluorescence/methods , Spermatozoa/physiology , Animals , Cryopreservation/methods , Dogs , Fluorescent Dyes/pharmacology , Male , Spermatozoa/drug effectsABSTRACT
Halo formation and phagocytic activity of leukocytes at different extreme states are regulated at the cellular level. Direct relationship between functional activity and halo formation is observed only during sublethal states in inflammatory diseases.
