ABSTRACT
Effect of the tretionine (retinoid) and aluminum chloride (neurotoxin) on the growth and differentiation of neuroblastoma cells in culture after their introduction into the medium separately and in combination was studied. The introduction of these substances creates a new information field in the medium, which becomes apparent by the reactions of neuroblastoma found on the populational and cellular levels of its organization. The presence of tretionine stimulates proliferation and induces differentiation of the cells into astrocytes. Aluminum chloride inhibits cell proliferation and enhances the process of their destruction in the monolayer. The variety of the reactions of neuroblastoma cells to the presence of these substances in the medium indicates the existence and functioning of a mechanism that selects from the information introduced only the portion which may contribute to adaptation of neuroblastoma cells to the changed culture conditions.
Subject(s)
Aluminum Compounds/pharmacology , Antineoplastic Agents/pharmacology , Chlorides/pharmacology , Neuroblastoma , Neuroepithelial Cells/drug effects , Neurotoxins/pharmacology , Tretinoin/pharmacology , Adaptation, Physiological , Aluminum Chloride , Animals , Astrocytes/cytology , Astrocytes/drug effects , Cell Differentiation/drug effects , Cell Division/drug effects , Cell Proliferation/drug effects , DNA/analysis , Information Theory , Neuroepithelial Cells/pathology , RNA/analysis , Tumor Cells, CulturedABSTRACT
The authors studied the pathology of bone marrow (BM) lymphoid cell from pigs infected by African swine fever virus (ASFV) in vitro. Monocytes were shown to be primarily afflicted in unstimulated BM culture. These cells disappeared completely 72 hours after infection. Just 24 hours following ASFV infection, there were atypical lymphocytes amounting to 12% of the general lymphoid population at hour 72 after inoculation.The area and perimeter of minor, middle, and large lymphocytes tended to reduce during both BM cell cultivation and inoculation. Lymphoblasts and monocytes were generally triploid in both the control and test groups, but among them there were diploid, triploid, and tetraploid cells. Cytophotometric assay revealed that the amount of nuclear DNA significantly increased in BM lymphoblasts and monocytes in the early stages of ASFV infection (within 24 hours). This effect was also rather pronounced in the lymphoblasts in the later stages (at hour 72).
Subject(s)
African Swine Fever/pathology , Lymphocytes/pathology , Lymphoid Tissue/pathology , Monocytes/pathology , African Swine Fever/immunology , African Swine Fever/virology , African Swine Fever Virus/physiology , Animals , Apoptosis , Bone Marrow/pathology , Cell Culture Techniques , Cell Nucleus/pathology , Cell Shape , DNA/analysis , Lymphocytes/immunology , Lymphoid Tissue/immunology , Monocytes/immunology , Swine , Tetraploidy , Time Factors , TriploidyABSTRACT
Changes of population and cellular parameters of HeLa and RD cultures after introducing of solcoseryl in culture medium were studied by methods of scanning cytophotometry and cytomorphometry. Monolayer density, proliferation activity, the number of dead cells in a monolayer, the number of nucleoli in nuclei and distribution of cells in the populations by this parameter, RNA and DNA masses in nuclei and nucleoli, total volumes and surface areas of the nuclei and nucleoli were determined. It has been shown that solcoseryl differently affects the cultures both on population and on cellular levels of their organization. The results of multi-parametric analysis of the influence of solseryl on the cultures allow considering it as a biologically active compound with the features typical for cell and cell population growth regulating factors.
Subject(s)
Actihaemyl/pharmacology , Cell Division/drug effects , Cell Line, Tumor , Cell Nucleolus/drug effects , Cell Nucleus/drug effects , Culture Media , Cytoplasm/drug effects , HeLa Cells , Humans , Time FactorsABSTRACT
The cells of nepatocarcinoma (HEp-G2), adenocarcinoma of large intestine (Caco-2), embryonal kidney (HEK-293), neuroblastoma (SH-SY5Y), rabdomyosarcoma (RD), and larynx cancer (Hep-2) were studied by the methods of scanning cytophotometry, cytochemistry and cytomorphometry during 96 h of cultivation. The density of monolayers, proliferation activity, the number of dead cells, DNA content in the nuclei and distribution of the cells in the population by this parameter, total DNA content in the nucleoli (circumnucleolar chromatin), the number of nucleoli in the nuclei, distribution of cells by their number, the volume and area of the nucleus surface, total volume and area of the nucleoli surface were determined. The data obtained were used in the treelike cluster analysis of the cultures by Pierson correlation. As a result, the SH-SY5Y culture was put in a separate cluster, while Caco-2, HEp-G2, HEK-293, Hep-2 and RD cultures were placed in the tree of another cluster. The least transformed culture of neuroblastoma (SH-SY5Y) had no relationship with other cultures, which showed various rate of similarity. The cultured HEK-293, Hep-2 and RD appeared to be close to each other by all parameters. The parameters studied are of different significance for the formation of general pattern of the cell cultures. The greatest "weight" is carried by the parameters, which characterize the population as whole: the density of the monolayer, mitotic coefficient and the number of dead cells. They are followed by the content of DNA in the nuclei, the total area of the nucleoli surface, and ratios of DNA content in the nucleoli to DNA content in the nucleus and of total surface of the nucleoli to the surface of the nuclei. Other parameters are not so significant.
