[PSYCHO-EDUCATIONAL PROGRAM IN PREVENTION OF MENTAL HEALTH OF INTERNALLY DISPLACED PERSONS].

The goal is to develop and evaluate the effectiveness of a psychoeducational program (PP) aimed at timely detection, prevention of the formation and prevention of chronicity of mental disorders in internally displaced persons (IDPs). 414 IDPs took part in the research. A complex of clinical-psychopathological, psychometric, psychodiagnostic and statistical methods were used. It was found that the group of IDPs is heterogeneous and includes 3 categories of persons: group I - IDPs without signs of mental disorders (conditionally healthy) (53,86%), group II - IDPs with individual symptoms of mental disorders (risk group) (20,05%) and group III - IDPs with established mental disorders (26,09%). The examined IDPs of the III group were found mental disorders, predominantly of the anxiety-depressive spectrum. Factors of psychic traumatization (FPT) were assessed in a dynamic aspect: retrospectively (during of staying in the anti-terrorist operation (ATO) zone), 12 and 24 months after leaving the ATO zone and during of examination. The dynamics of FPT and their significance in time and with regard to gender specificity were shown. In order to timely identify and prevent the formation of mental disorders in IDPs and increase their adaptive capabilities, it is proposed to use PP, which had a group structured work format. The results of its approbation were presented and it were proved that the using of PP could significantly increase the level of psychological adaptation of IDPs by reducing the level of mental stress, reactive anxiety and the severity of negative perception of the future and improving the quality of life.

Detection of Small Subsets of CD4+ Lymphocytes with SmartFlare Nanoprobes.

SmartFlare technology allows detection of mRNA in single living cells. We studied the possibility of using SmartFlare nanoprobes for detection of small subsets of CD4+ lymphocytes. It was found that SmartFlare allows detection of transcriptional master regulators of major CD4+T helper subsets in living human lymphocytes. Nanoprobes labeled with Cy5 fluorophore were better detected by flow cytometry than nanoprobes labeled with Cy3. Appropriate time of lymphocyte incubation with SmartFlare probes was 24 h.

[STRUCTURAL ABNORMALITIES OF THE OBSTRUCTED URETEROVESICAL JUNCTION].

The article presents a morphological study of UVJ removed during surgery in 110 children with stage III hydronephrosis. Morphological changes in resected UVJs indicate a predominance of sclerotic transformation accompanied by different types of dysplasia.

[Features of patients with the multiple sensitization diagnostics before allergen specific immunotherapy will be set and the assessment of the therapy results with the laboratory methods].

BACKGROUND: Due to the wide expansion of atopy, its early beginning, variety of forms, difficulty of specific pathogenetic treatment, and also high cost of in vitro researches there is a need of diagnostic test systems development and optimization. AIM: To make the assessment of atopy diagnostics efficiency in children with a multiple allergy and the analysis of specific immunotherapy (ASIT) influence on immune markers level in serum for a choice of the most significant predictive indicator. PATIENTS AND METHODS: 457 children (mean age 8,9 ± 4,3 years) with pollinosis symptoms that prevalence in spring period were tested with birch pollen allergens extract by detecting allergen-specific immunoglobulin E (sIgE) levels. Than patient witch showed positive sIgE level (243 children) were tested with expanded set of plant allergens (birch, alder, hazel, oak pollen, allergens of Rosaceae family and carrot) and set of birch pollen allergocomponents (Bet v1, Bet v2, Bet v4, Bet v6). From them 32 patients were treated with allergen-specific immunotherapy. Immunological assays were performed by indirect immunofluorescent method on ImmunoCAP250 (Sweden). RESULTS: It was shown that birch allergens sIgE antibodies detection in patients with pollinosis allows to estimate sensitization degree to allergens of related trees and could predict their quantitative values. The oak allergens sIgE level is a good predictive marker of sIgE level to food plant derived allergens. And apple allergens sIgE concentration is closely assotiated with sIgE to fruit allergens of Rosacea family. Detection of sensitization to minor allergens in patient influences on therapy efficacy prognosis. CONCLUSION: sIgE detection to limited number of allergens (birch-oak-apple) is effective to sIgE value assessment in patient with allergy to plant causing allergens cross reactivity. Component-divided in vitro diagnostics directed on reveal of sensitization caused by minor allergens, is actual at the answer a question about ASIT validity and its efficiency. Component-divided in vitro diagnostics directed on reveal of sensitization caused by minor allergens, is actual at the answer a question about ASIT validity and its efficiency. Significant results of the therapy are shown after double course ASIT that also allows to reduce considerably production of sIgE antibodies to significant allergens, and cross reacting plant food allergens.

Cytokine profile in children with inflammatory bowel disease.

Crohn's disease (CD) and ulcerative colitis (UC) are inflammatory bowel diseases (IBD) characterized by immune reactivity against microbial and auto-antigens. This work was designed to study the cytokine profile in blood serum and coproextracts of children with CD and UC. The studied patients consisted of 17 children with CD (group I), 17 children with UC (group II), and 18 controls with intestinal dysbiosis (group III). The diagnosis of UC and CD was based on accepted clinical and endoscopic criteria. The levels of 13 cytokines (IL-1ß, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12p70, IL-17A, TNF-α, TGF-ß, and IFN-γ) were determined in blood sera and coproextracts of the patients and controls using the BioPlex technology. The level of IL-17A was significantly increased and that of TGF-ß was significantly decreased in the blood serum of the patients with IBDs. Changes in the cytokine profile in the coproextracts affected the wider spectrum of cytokines. The levels of proinflammatory cytokines (IL-2, IL-4, IL-6, IL-12p70, TNF-α, and IFN-γ) were increased 6-9-fold, whereas the level of the anti-inflammatory cytokine IL-10 was increased 3-fold. The cytokine balance was shifted to the proinflammatory cytokines. The TGF-ß level was increased 9-fold and that of IL-17A was increased 3-fold. Thus, the cytokine profile in the coproextracts was more informative than that of the blood serum. The determination of cytokines in coproextracts is simple and noninvasive.

[Immunophenotypic characteristics of blood lymphocytes and expression of cytokine receptors in newborn infants].

Formation of the immune system during the neonatal period was studied in 88 infants aged 3 days-1 month (gestation age 28-41 weeks) with the primary diagnosis of cerebral ischemia. The patients were allocated to 4 groups based on chronological and gestational age at the moment of examination and a reference group. A total of 16 umbilical blood samples were available for analysis. Phenotype of blood lymphocyte was identified by flow cytofluorimetry with differentiation between subpopulations of T and B lymphocytes and detection of membrane receptors of IL-2, 8, and 10. T-cell subpupulation was found to be enriched in CD3+CD4+CD8+ lymphocytes. Content of B1 lymphocytes was equally high in full-term and premature infants. The latter showed enhanced expression of IL-2 receptor, CD122, by the end of the first month of life. Expression of receptors of IL-8 (chemotaxis factor) and IL-10 was high in lymphocytes of children of all groups since the onset of the neonatal period. It is concluded that comprehensive study of membrane cytokine receptors is of interest for better understanding mechanisms underlying maturation of the immune system in children of different age groups.

[In vitro cell adhesion and integrin expression by calcium ionophore-treated mononuclear cells from patients with acute myeloid leukemia]. / Adgezivnye svoistva i ékspressiia integrinov kletkami bol'nykh ostrymi mieloidnymi leikozami, stimulivannymi v kul'ture ionoforom dlia ionov kal'tsiia.

As shown elsewhere, cultured acute myeloid leukaemia blasts acquire certain characteristics of dendritic cells upon stimulation with cytokines and calcium ionophore. The ability of leukaemia-derived dendritic-like cells to express immune costimulatory molecules and dendritic cell marker CD83 has been extensively investigated. Although migratory capacity is a major attribute of dendritic cells, the ability of in vitro modified blasts for adhesion, chemotaxis and homing remain elusive. In the present paper, we show that after stimulation with calcium ionophore acute myeloid leukaemia blasts as well as normal dendritic cell precursors demonstrate increased capacity of binding fibronectin and denatured collagen. The expression pattern of integrins on dendritic-like leukaemic cells in general closely resembles that of monocyte-derived dendritic cells, however, variation in cell properties isolated from blood of individual patients are observed.

The changes of lymphocyte membrane receptors in bronchial asthma and atopic dermatitis in pediatric patients receiving treatment with polyenic fatty acids.

The influence of a diet supplemented with n-3 PUFA on the immune status of children with atopic dermatitis and asthma was investigated. The results of the investigation have shown the improvement of cell immunity along with a decrease in the clinical manifestation of the disease. n-3 PUFA could be used as immunocorrectors in combination with pathogenic treatment of children with allergic diseases.

[Pathogenesis of impaired cardiac electric stability in experimental hypodynamia]. / K voprosu o patogeneze narushenii élektricheskoi stabil'nosti serdtsa pri éksperimental'noi gipodinamii.

The decreased ventricular fibrillation threshold (VFT) in the first 5 days of restriction results from stress-induced changes in myocardial autonomic innervation, as shown by elevated cardiac content of cAMP and lowered levels of cGMP, and a rise in the plasma concentrations of corticosterone and deoxycorticosterone. Ultimately, these changes enhance cardiomyocyte membranous permeability of calcium ions and, hence, lower VFT. Impaired cardiac electric stability in the late inactivity period (50 days) is also associated with the enhanced permeability of cardiomyocyte membranes; however, the nature of this phenomenon remains unclear and calls for further studies.

[Membrane markers and interleukin-2 receptor of splenic lymphocytes in children with aplastic anemia: detection of active subpopulations]. / Membrannye markery i retseptor k interleikinu-2 limfotsitov selezenki u detei s aplasticheskoi anemiei: vyiavlenie aktivnykh sub- populiatsii.

A total of 16 children with aplastic anemia were investigated, among them 6 girls and 10 boys, the mean age 10.5 years. Splenectomy was included into the complex of treatment along with glucocorticoid and transfusion-replacement therapy of all the children. The membrane markers of splenic lymphocytes were assayed in the indirect immunofluorescence test with monoclonal antibodies of ICO and OKT series. The mean parameters did not significantly differ from those of subpopulations of splenic lymphocytes in the control group described in the literature. An individual analysis made in 8 patients (50%) has revealed active lymphocyte populations in the spleen: in the group of patients with a favourable prognosis to the disease an increase in the percent of B-cells was recorded in 2, OKT8(+)-lymphocytes in 1 patient; a rise in the percent of cells with receptors to interleukin-2 in 3 patients (from 25 to 32%); in the group of patients with severe aplasia an increase in the percent of activated HLA-DR(+)-lymphocytes was recorded in 2, and OKT8(+)-lymphocytes in 1 patient. All the patients responded well to the treatment. It has been suggested that in all cases the splenectomy effect is associated with the removal of immunoactive lymphocytes and can serve as an equivalent of the immunosuppressive therapy. The effect of splenectomy in the rest cases has been obscure.