Effects of a US Supreme Court ruling to restrict abortion rights.

Previous research focused on popular US Supreme Court rulings expanding rights; however, less is known about rulings running against prevailing public opinion and restricting rights. We examine the impact of the Dobbs v. Jackson Women's Health Organization opinion, which overturned Roe v. Wade's (1973) constitutional protection of abortion rights. A three-wave survey panel (5,489 interviews) conducted before the leak of the drafted Dobbs opinion, after the leak, and after the official opinion release, and cross-sectional data from these three time points (10,107 interviews) show that the ruling directly influenced views about the constitutional legality of abortion and fetal viability. However, personal opinions were not directly influenced and perceived social norms shifted away from the ruling, meaning that individuals perceived greater public support for abortion. We argue that extensive coverage of opposition to overturning Roe v. Wade supported this shift. Dobbs v. Jackson Women's Health Organization also caused large changes, polarized by party identification, in opinions about the Supreme Court.

Save the Supreme Court and democracy.

The US Supreme Court has been busy. It recently overturned a nearly 50-year-old precedent protecting abortion rights, upheld the right to carry guns outside the home, and hamstrung the Environmental Protection Agency's ability to regulate emissions-all while signaling an aversion to contemporary empirical evidence and instead favoring "history and tradition." Although the majority of Americans disagree with many of these decisions, the court has only just begun to reshape the country. When it resumes in October, the court will be poised to outlaw affirmative action, undercut federal regulations regarding clean water, and possibly allow state legislatures to restrict voting rights without oversight by state courts. What explains the court's shift to an ideological extreme, and what can be done about it?

A decade-long longitudinal survey shows that the Supreme Court is now much more conservative than the public.

Has the US Supreme Court become more conservative than the public? We introduce results of three surveys conducted over the course of a decade that ask respondents about their opinions on the policy issues before the court. Using these data, we show that the gap between the court and the public has grown since 2020, with the court moving from being quite close to the average American to a position that is more conservative than the majority of Americans. Second, in contrast to findings showing consistency in the public's approval of or deference to the court, we find that the public's expectations of the court vary significantly over time and in tandem with changes in the court's composition and recent rulings. Even so, many members of the public currently underestimate the court's conservative leaning. Third, we find that respondents' perceptions of the court's ideology relative to their own are associated with support for institutional changes but with important differences between Democrats and Republicans. The fact that so many people currently underestimate how conservative the court is implies that support for proposed changes to the court may be weaker than it would be if people knew with greater accuracy the court's conservative nature.

Strand-specific single-cell methylomics reveals distinct modes of DNA demethylation dynamics during early mammalian development.

DNA methylation (5mC) is central to cellular identity. The global erasure of 5mC from the parental genomes during preimplantation mammalian development is critical to reset the methylome of gametes to the cells in the blastocyst. While active and passive modes of demethylation have both been suggested to play a role in this process, the relative contribution of these two mechanisms to 5mC erasure remains unclear. Here, we report a single-cell method (scMspJI-seq) that enables strand-specific quantification of 5mC, allowing us to systematically probe the dynamics of global demethylation. When applied to mouse embryonic stem cells, we identified substantial cell-to-cell strand-specific 5mC heterogeneity, with a small group of cells displaying asymmetric levels of 5mCpG between the two DNA strands of a chromosome suggesting loss of maintenance methylation. Next, in preimplantation mouse embryos, we discovered that methylation maintenance is active till the 16-cell stage followed by passive demethylation in a fraction of cells within the early blastocyst at the 32-cell stage of development. Finally, human preimplantation embryos qualitatively show temporally delayed yet similar demethylation dynamics as mouse embryos. Collectively, these results demonstrate that scMspJI-seq is a sensitive and cost-effective method to map the strand-specific genome-wide patterns of 5mC in single cells.

Does Encouragement Matter in Improving Gender Imbalances in Technical Fields? Evidence from a Randomized Controlled Trial.

Does encouragement help address gender imbalances in technical fields? We present the results of one of the first and largest randomized controlled trials on the topic. Using an applied statistics conference in the social sciences as our context, we randomly assigned half of a pool of 3,945 graduate students to receive two personalized emails encouraging them to apply (n = 1,976) and the other half to receive nothing (n = 1,969). We find a robust, positive effect associated with this simple intervention and suggestive evidence that women responded more strongly than men. However, we find that women's conference acceptance rates are higher within the control group than in the treated group. This is not the case for men. The reason appears to be that female applicants in the treated group solicited supporting letters at lower rates. Our findings therefore suggest that "low dose" interventions may promote diversity in STEM fields but may also have the potential to expose underlying disparities when used alone or in a non-targeted way.

The ClpXP protease unfolds substrates using a constant rate of pulling but different gears.

ATP-dependent proteases are vital to maintain cellular protein homeostasis. Here, we study the mechanisms of force generation and intersubunit coordination in the ClpXP protease from E. coli to understand how these machines couple ATP hydrolysis to mechanical protein unfolding. Single-molecule analyses reveal that phosphate release is the force-generating step in the ATP-hydrolysis cycle and that ClpXP translocates substrate polypeptides in bursts resulting from highly coordinated conformational changes in two to four ATPase subunits. ClpXP must use its maximum successive firing capacity of four subunits to unfold stable substrates like GFP. The average dwell duration between individual bursts of translocation is constant, regardless of the number of translocating subunits, implying that ClpXP operates with constant "rpm" but uses different "gears."

ClpX(P) generates mechanical force to unfold and translocate its protein substrates.

AAA(+) unfoldases denature and translocate polypeptides into associated peptidases. We report direct observations of mechanical, force-induced protein unfolding by the ClpX unfoldase from E. coli, alone, and in complex with the ClpP peptidase. ClpX hydrolyzes ATP to generate mechanical force and translocate polypeptides through its central pore. Threading is interrupted by pauses that are found to be off the main translocation pathway. ClpX's translocation velocity is force dependent, reaching a maximum of 80 aa/s near-zero force and vanishing at around 20 pN. ClpX takes 1, 2, or 3 nm steps, suggesting a fundamental step-size of 1 nm and a certain degree of intersubunit coordination. When ClpX encounters a folded protein, it either overcomes this mechanical barrier or slips on the polypeptide before making another unfolding attempt. Binding of ClpP decreases the slip probability and enhances the unfolding efficiency of ClpX. Under the action of ClpXP, GFP unravels cooperatively via a transient intermediate.

Phase behavior of repulsive polymer-tethered colloids.

We report molecular dynamics simulations of a system of repulsive, polymer-tethered colloidal particles. We use an explicit polymer model to explore how the length and the behavior of the polymer (ideal or self-avoiding) affect the ability of the particles to organize into ordered structures when the system is compressed to moderate volume fractions. We find a variety of different phases whose origin can be explained in terms of the configurational entropy of polymers and colloids. Finally, we discuss and compare our results to those obtained for similar systems using simplified coarse-grained polymer models, and set the limits of their applicability.

A longitudinal study of gendered vocabulary and communicative action in young children.

Development of children's vocabularies for gender-typed words and communicative actions was investigated longitudinally from 13 to 36 months and in a group of 9.5-month-olds. Vocabularies of gendered words were assessed using lists of adult-rated gender-typed words from the MacArthur-Bates Communicative Development Inventories (CDI; L. Fenson et al., 1994). At 24 to 36 months, girls' and boys' productive vocabularies contained more same-gender-typed words than other-gender-typed words. Receptive vocabulary gender-differential effects were apparent among boys at 18 months. At 13 and 18 months, gender-typed differences were apparent in communicative actions. The research reveals the utility of unobtrusive, nonexperimental measures for assessing gender-related knowledge and behavior in young children.