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1.
J Assist Reprod Genet ; 36(12): 2419, 2019 Dec.
Article in English | MEDLINE | ID: mdl-31820310

ABSTRACT

The original article unfortunately contained a mistake. In the online version of the paper, the words "MII (metaphase II)-PB1 (1st polar body) complex (MII-PB1 complex)" in table 1 are incorrectly placed.

2.
J Assist Reprod Genet ; 36(12): 2403-2418, 2019 Dec.
Article in English | MEDLINE | ID: mdl-31705227

ABSTRACT

PURPOSE: Molecular cytogenetic analysis has confirmed that a proportion of apparently meiotic aneuploidy may be present in the germ cells prior to the onset of meiosis, but there is no clear perception of its frequency. The aim of this review is to assess the evidence for premeiotic aneuploidy from a variety of sources to arrive at an estimate of its overall contribution to oocyte aneuploidy in humans. METHODS: Relevant scientific literature was covered from 1985 to 2018 by searching PubMed databases with search terms: gonadal/germinal mosaicism, ovarian mosaicism, premeiotic aneuploidy, meiosis and trisomy 21. Additionally, a key reference from 1966 was included. RESULTS: Data from over 9000 cases of Down syndrome showed a bimodal maternal age distribution curve, indicating two overlapping distributions. One of these matched the pattern for the control population, with a peak at about 28 years and included all cases that had occurred independently of maternal age, including those due to germinal mosaicism, about 40% of the cohort. The first cytological proof of germinal mosaicism was obtained by fluorescence in situ hybridisation analysis. Comparative genomic hybridisation analysis of oocyte chromosomes suggests an incidence of up to 15% in premeiotic oocytes. Direct investigation of fetal ovarian cells led to variable results for chromosome 21 mosaicism. CONCLUSIONS: Oocytes with premeiotic errors will significantly contribute to the high level of preimplantation and prenatal death. Data so far available suggests that, depending upon the maternal age, up to 40% of aneuploidy that is present in oocytes at the end of meiosis I may be due to germinal mosaicism.


Subject(s)
Aneuploidy , Chromosomes/genetics , Meiosis/genetics , Mosaicism , Comparative Genomic Hybridization , Female , Germ Cells/growth & development , Germ Cells/pathology , Humans , Maternal Age , Oocytes/growth & development , Oocytes/pathology , Pregnancy
3.
Syst Biol Reprod Med ; 62(6): 415-422, 2016 Dec.
Article in English | MEDLINE | ID: mdl-27686340

ABSTRACT

Preimplantation embryos may have an increased risk of having mismatches due to the rates of cell proliferation and DNA replication. Elimination of mismatches in human gametes and embryos has not been investigated. In this study we developed a sensitive functional assay to examine the repair or elimination of mismatches in both commercially available cell extracts and extracts obtained from preimplantation embryos. Heteroduplex molecules were constructed using synthetic oligonucleotides. Efficiency of the repair of mismatches was semi-quantitatively analysed by exposure to nuclear/whole cell extracts (as little as 2.5 µg) and extracts obtained from pooled mouse and human blastocysts to investigate the repair capacity in human embryos. A cell free in vitro assay was successfully developed to analyze the repair of mismatches using heteroduplex complexes. The assay was further optimized to analyze repair of mismatches in cell extracts obtained from oocytes and blastocysts using minute amounts of protein. The efficiency of mismatch repair was examined in both mouse and human blastocysts (2.5 µg). The blastocysts were observed to have a lower repair efficiency compared to commercially available nuclear and whole cell extracts. In conclusion, a sensitive, easy, and fast in vitro technique was developed to detect the repair of mismatch efficiency in embryos.


Subject(s)
Base Pair Mismatch , Blastocyst/metabolism , Cell Extracts , Cell Nucleus/metabolism , Animals , DNA Repair , Humans , Mice
4.
Eur J Hum Genet ; 25(1): 37-42, 2016 01.
Article in English | MEDLINE | ID: mdl-27677417

ABSTRACT

Gene expression from both parental genomes is required for completion of embryogenesis. Differential methylation of each parental genome has been observed in mouse and human preimplantation embryos. It is possible that these differences in methylation affect the level of gene transcripts from each parental genome in early developing embryos. The aim of this study was to investigate if there is a parent-specific pattern of BRCA1 expression in human embryos and to examine if this affects embryo development when the embryo carries a BRCA1 or BRCA2 pathogenic mutation. Differential parental expression of ACTB, SNRPN, H19 and BRCA1 was semi-quantitatively analysed by minisequencing in 95 human preimplantation embryos obtained from 15 couples undergoing preimplantation genetic diagnosis. BRCA1 was shown to be differentially expressed favouring the paternal transcript in early developing embryos. Methylation-specific PCR showed a variable methylation profile of BRCA1 promoter region at different stages of embryonic development. Embryos carrying paternally inherited BRCA1 or 2 pathogenic variants were shown to develop more slowly compared with the embryos with maternally inherited BRCA1 or 2 pathogenic mutations. This study suggests that differential demethylation of the parental genomes can influence the early development of preimplantation embryos. Expression of maternal and paternal genes is required for the completion of embryogenesis.


Subject(s)
BRCA1 Protein/genetics , BRCA2 Protein/genetics , DNA Methylation/genetics , Embryonic Development/genetics , Alleles , Animals , Blastocyst/metabolism , Female , Gene Expression Regulation, Developmental , Genomic Imprinting/genetics , Humans , Maternal Inheritance/genetics , Mice , Mutation , Parents , Pregnancy
5.
Prenat Diagn ; 36(9): 864-70, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27441947

ABSTRACT

OBJECTIVES: Mosaicism in certain dominant disorders may result in a 'non-Mendelian' transmission for the causative mutation. Preimplantation genetic diagnosis (PGD) is available for patients with inherited disorders to achieve an unaffected pregnancy. We present our experience for two female patients with different dominantly inherited autosomal disorders; neurofibromatosis type 1 (NF1) and tuberous sclerosis complex type 2 (TSC2). METHODS: PGD protocol development was carried out using single cells from the patients. PGD was carried out on polar bodies and different embryonic cells. RESULTS: Protocol development for NF1 using lymphocytes from the patient suggested mosaicism for the mutation. This was supported further by quantitative fluorescent-PCR performed on genomic DNA. During PGD, polar bodies and blastomeres lacked the mutation that probably was absent or present at very low levels in the patient's germline. Single lymphocyte analysis during protocol development for TSC2 did not indicate mosaicism; however, analysis of single buccal cells and multiple embryo biopsies across two consecutive IVF/PGD cycles confirmed gonosomal mosaicism. CONCLUSIONS: The trend in PGD is for blastocyst biopsy followed by whole genome amplification, eliminating single cell analysis. In the case of certain dominantly inherited disorders, pre-PGD single cell analysis is beneficial to identify potential mosaicism that ensures robust protocols. © 2016 John Wiley & Sons, Ltd.


Subject(s)
Mosaicism , Neurofibromatosis 1/diagnosis , Preimplantation Diagnosis , Tuberous Sclerosis/diagnosis , Adult , Female , Humans , Pregnancy
6.
J Reprod Dev ; 62(3): 225-34, 2016 Jun 17.
Article in English | MEDLINE | ID: mdl-26853522

ABSTRACT

Active DNA repair pathways are crucial for preserving genomic integrity and are likely among the complex mechanisms involved in the normal development of preimplantation embryos. MicroRNAs (miRNA), short non-coding RNAs, are key regulators of gene expression through the post-transcriptional and post-translational modification of mRNA. The association of miRNA expression with infertility or polycystic ovarian syndrome has been widely investigated; however, there are limited data regarding the importance of miRNA regulation in DNA repair during preimplantation embryo development. In this article, we review normal miRNA biogenesis and consequences of aberrant miRNA expression in the regulation of DNA repair in gametes and preimplantation embryos.


Subject(s)
Blastocyst/cytology , DNA Repair , MicroRNAs/metabolism , Animals , Embryo Culture Techniques , Embryonic Development , Female , Fertilization , Gene Expression Regulation, Developmental , Humans , Male , MicroRNAs/genetics , Oocytes/cytology , Phenotype , Pregnancy , Pregnancy, Animal , Protein Processing, Post-Translational
7.
Reprod Biomed Online ; 28(5): 624-37, 2014 May.
Article in English | MEDLINE | ID: mdl-24581987

ABSTRACT

This is a retrospective study aiming to assess telomere length in human embryos 4 days post fertilization and to determine whether it is correlated to chromosomal ploidy, embryo developmental rate and patient age. Embryos were donated from patients undergoing treatment in the assisted conception unit. Seven couples took part, generating 35 embryos consisting of 1130 cells. Quantitative fluorescent in-situ hybridization (FISH) measured the telomere length of every cell using a pan-telomeric probe. Conventional FISH on six chromosomes was used to assess aneuploidy in the same cells. Maternal and paternal age, referral reason, embryo developmental rate and type of chromosomal error were taken into account. Chromosomally abnormal cells were associated with shorter telomeres than normal cells for embryos that were developmentally slow. Cells produced by women of advanced maternal age and those with a history of repeated miscarriage tended to have substantially shorter telomeres. There was no significant difference in telomere length with respect to the rate of embryo development 5 days post fertilization. Telomeres play an important role in cell division and shorter telomeres may affect embryonic ploidy. Reduced telomere length was associated with aneuploid cells and embryos from women of advanced maternal age.


Subject(s)
Blastocyst/metabolism , Telomere/physiology , Adult , Aneuploidy , Cells, Cultured , Chromosome Aberrations/embryology , Chromosome Aberrations/statistics & numerical data , Embryo Culture Techniques , Embryonic Development/genetics , Female , Humans , In Situ Hybridization, Fluorescence , Lymphocytes/cytology , Lymphocytes/metabolism , Male , Retrospective Studies
8.
Fertil Steril ; 99(3): 803-814.e23, 2013 Mar 01.
Article in English | MEDLINE | ID: mdl-23148922

ABSTRACT

OBJECTIVE: To compare the oocyte versus the blastocyst transcriptome and provide data on molecular pathways before and after embryonic genome activation. DESIGN: Prospective laboratory research study. SETTING: An IVF clinic and a specialist preimplantation genetics laboratory. PATIENT(S): Couples undergoing or having completed IVF treatment donating surplus oocytes or cryopreserved blastocysts after patient consent. INTERVENTION(S): Sets of pooled metaphase II (MII) oocytes or blastocysts were processed for RNA extraction, RNA amplification, and analysis with the use of the Human Genome Survey Microarrays v2.0 (Applied Biosystems). MAIN OUTCOME MEASURE(S): Association of cell type and gene expression profile. RESULT(S): Totals of 1,909 and 3,122 genes were uniquely expressed in human MII oocytes and human blastocysts respectively, and 4,910 genes were differentially expressed between the two sample types. Expression levels of 560 housekeeping genes, genes involved in the microRNA processing pathway, as well as hormones and hormone receptors were also investigated. CONCLUSION(S): The lists of genes identified may be of use for understanding the processes involved in early embryo development and blastocyst implantation, and for identifying any dysregulation leading to infertility.


Subject(s)
Blastocyst/physiology , Embryonic Development/genetics , Gene Expression Regulation, Developmental/genetics , Oocytes/physiology , Transcriptome , Cryopreservation , Female , Fertilization in Vitro , Genome, Human/genetics , Humans , Male , Meiosis/genetics , MicroRNAs/genetics , Prospective Studies , Real-Time Polymerase Chain Reaction
9.
Expert Rev Mol Diagn ; 12(6): 585-92, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22845479

ABSTRACT

Over the last 20 years, preimplantation genetic diagnosis (PGD) has changed from being an experimental procedure to one that is carried out in specialized diagnostic centers worldwide. Genetic awareness and the rapid identification of germline mutations or chromosomal abnormalities enable individuals to know their risk of transmitting a genetic disease before they have children. This has created a demand for PGD from couples who wish to avoid terminations of affected pregnancies. Although PGD is expensive because it requires couples to go through IVF, there is a trend for diagnosis to move towards automation, which will reduce cost and the need for specialized expertise. This will allow diagnosis to be carried out in routine molecular diagnostic laboratories.


Subject(s)
Preimplantation Diagnosis/methods , Fertilization in Vitro , Genetic Testing/methods , Genomics/methods , Humans
11.
Hum Genet ; 131(2): 175-86, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21748341

ABSTRACT

For the last 20 years, preimplantation genetic diagnosis (PGD) has been mostly performed on cleavage stage embryos after the biopsy of 1-2 cells and PCR and FISH have been used for the diagnosis. The main indications have been single gene disorders and inherited chromosome abnormalities. Preimplantation genetic screening (PGS) for aneuploidy is a technique that has used PGD technology to examine chromosomes in embryos from couples undergoing IVF with the aim of helping select the chromosomally 'best' embryo for transfer. It has been applied to patients of advanced maternal age, repeated implantation failure, repeated miscarriages and severe male factor infertility. Recent randomised controlled trials (RCTs) have shown that PGS performed on cleavage stage embryos for a variety of indications does not improve delivery rates. At the cleavage stage, the cells biopsied from the embryo are often not representative of the rest of the embryo due to chromosomal mosaicism. There has therefore been a move towards blastocyst and polar body biopsy, depending on the indication and regulations in specific countries (in some countries, biopsy of embryos is not allowed). Blastocyst biopsy has an added advantage as vitrification of blastocysts, even post biopsy, has been shown to be a very successful method of cryopreserving embryos. However, mosaicism is also observed in blastocysts. There have been dramatic changes in the method of diagnosing small numbers of cells for PGD. Both array-comparative genomic hybridisation and single nucleotide polymorphism arrays have been introduced clinically for PGD and PGS. For PGD, the use of SNP arrays brings with it ethical concerns as a large amount of genetic information will be available from each embryo. For PGS, RCTs need to be conducted using both array-CGH and SNP arrays to determine if either will result in an increase in delivery rates.


Subject(s)
Blastocyst , Polar Bodies , Preimplantation Diagnosis/methods , Biopsy , Chromosomes/chemistry , Comparative Genomic Hybridization , Embryo, Mammalian/pathology , Female , Genetic Testing , Humans , Molecular Diagnostic Techniques , Nucleic Acid Amplification Techniques , Polymorphism, Single Nucleotide , Pregnancy
12.
Fertil Steril ; 94(5): 1674-9, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20171614

ABSTRACT

OBJECTIVE: To overcome problems associated with the use of triplet repeat primed polymerase chain reaction (TP-PCR) in preimplantation genetic diagnosis (PGD) of myotonic dystrophy type 1 (DM1). DESIGN: Clinical research study. SETTING: UCL Centre for PGD and Centre for Reproductive and Genetic Health. PATIENT(S): Seven couples undergoing PGD for DM1. INTERVENTION(S): A modified TP-PCR protocol (mTP-PCR) for the reliable detection of both expanded and nonexpanded alleles in DMPK was optimized using single lymphocytes. Four cycles of PGD were performed with TP-PCR for diagnosis and a further 10 cycles with mTP-PCR. MAIN OUTCOME MEASURE(S): Amplification efficiency, allele dropout, diagnosis rate, and delivery rate. RESULT(S): Preliminary testing showed that the TP-PCR amplification efficiency was higher using lymphocytes versus buccal cells. Single lymphocytes gave very high amplification efficiencies for both protocols (99% to 100%). There were no false-positive or false-negative results for 148 single lymphocytes tested with mTP-PCR compared with 9% (5 out of 54) false-positive results with TP-PCR, indicating the improved accuracy of the modified protocol. In embryos, the diagnosis rate was 95.6% with mTP-PCR and 75% with TP-PCR. CONCLUSION(S): For PGD of DM1, mTP-PCR is recommended. It may also be applied as a rapid screen for DMPK expansions in individuals with symptoms of DM1, relatives of known mutation carriers, or in prenatal diagnosis.


Subject(s)
Myotonic Dystrophy/diagnosis , Myotonic Dystrophy/genetics , Polymerase Chain Reaction/methods , Preimplantation Diagnosis/methods , Trinucleotide Repeats/genetics , Alleles , Base Sequence , False Negative Reactions , False Positive Reactions , Female , Genetic Testing , Humans , Lymphocytes , Male , Molecular Sequence Data , Myotonic Dystrophy/classification , Myotonin-Protein Kinase , Nucleic Acid Amplification Techniques , Protein Serine-Threonine Kinases/genetics
13.
Hum Reprod ; 24(10): 2649-55, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19542543

ABSTRACT

BACKGROUND: The early preimplantation embryo relies on mRNA and protein from the oocyte to detect DNA damage and activate DNA repair, cell cycle arrest or apoptosis. Expression of some repair genes has been detected in mammalian oocytes and embryos; however, little is known about DNA repair gene expression in human blastocysts. In this study, DNA repair gene expression was investigated in human oocytes and blastocysts to identify the pathways involved at these stages and detect potential differences in repair mechanisms pre- and post-embryonic genome activation. METHODS: Triplicate sets of pooled metaphase II oocytes or blastocysts were processed for analysis using the Human Genome Survey Microarrays V2.0 (Applied Biosystems). RESULTS: Of 154 DNA repair genes investigated, 109 were detected in blastocysts and 107 in oocytes. Among differentially expressed DNA repair genes, 40/55 (73%) had lower expression levels in blastocysts compared with oocytes (P < 0.05, fold change >3). CONCLUSION: Despite experimental limitations due to culture or freezing and thawing of samples, large numbers of repair genes were detected indicating that all DNA repair pathways are potentially functional in human oocytes and blastocysts. The higher mRNA level for most repair genes in oocytes compared with blastocysts ensures sufficient availability of template until embryonic genome activation.


Subject(s)
Blastocyst/metabolism , DNA Repair/genetics , Oocytes/metabolism , DNA Repair/physiology , Gene Expression Profiling , Humans , Oligonucleotide Array Sequence Analysis , RNA, Messenger/metabolism
14.
Neuromuscul Disord ; 18(2): 131-6, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18053720

ABSTRACT

Myotonic dystrophy type 1 (DM1) is a dominant multisystemic disorder caused by expansion of a trinucleotide repeat in a non-coding region of DMPK. Prenatal diagnosis (PND) is available; however, the decision to terminate affected pregnancies is difficult as the extent of disability is hard to predict from the size of the expansion. In preimplantation genetic diagnosis (PGD) genetic analysis is carried out before the establishment of pregnancy. This paper reviews the largest number of cycles of PGD for DM1 in the UK indicating that PGD is a practical option for affected couples.


Subject(s)
Genetic Testing , Myotonic Dystrophy/diagnosis , Myotonic Dystrophy/genetics , Preimplantation Diagnosis , Protein Serine-Threonine Kinases/genetics , Female , Fertilization in Vitro , Humans , Male , Myotonin-Protein Kinase , Polymerase Chain Reaction , Trinucleotide Repeats , United Kingdom
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